干旱区生态环境知识资源中心(Arid): Tracking tick-borne diseases in Mongolian livestock using next generation sequencing (NGS)

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DOI	10.1016/j.ttbdis.2021.101845
	Tracking tick-borne diseases in Mongolian livestock using next generation sequencing (NGS)
	Chaorattanakawee, Suwanna; Wofford, Rachel N.; Takhampunya, Ratree; Poole-Smith, B. Katherine; Boldbaatar, Bazartseren; Lkhagvatseren, Sukhbaatar; Altantogtokh, Doniddemberel; Musih, Elisha; Nymadawa, Pagbajab; Davidson, Silas; Hertz, Jeffrey; Fiorenzano, Jodi; Gray, Gregory C.; von Fricken, Michael E.
通讯作者	von Fricken, ME (corresponding author), George Mason Univ, Dept Global & Community Hlth, 4400 Univ Dr, Fairfax, VA 22030 USA.
来源期刊	TICKS AND TICK-BORNE DISEASES
ISSN	1877-959X
EISSN	1877-9603
出版年	2022
卷号	13 期号:1
英文摘要	The livestock industry in Mongolia accounts for 24% of national revenue, with one third of the population maintaining a pastoral lifestyle. This close connection between Mongolian population and livestock is a major concern for pathogen transfer, especially given the increase in vector-borne diseases globally. This study examines blood samples from livestock to assess the prevalence of tick-borne bacterial infections across three provinces in Mongolia (Dornogovi, Selenge, To center dot v). Whole blood samples from 243 livestock (cattle=38, camel=11, goat=85, horse=22, sheep=87) were analyzed with 16S metagenomics next-generation sequencing (NGS) to screen for bacterial pathogens. Positive-NGS samples for Anaplasma, Bartonella, Ehrlichia, Francisella, Leptospira, and Rickettsia were confirmed by conventional PCR and DNA sequencing. Prevalence rates of Anaplasma, Bartonella, and Ehrlichia were 57.6%, 12.8%, and 0.4%, respectively. A significant difference in the prevalence of Anaplasma spp. in livestock by province was observed, with a higher prevalence in Selenge (74.2%, p<0.001) and To center dot v (64.2% p = 0.006) compared to the semi-arid region of Dornogovi (39.8%). In contrast, no association was observed in Bartonella prevalence by provinces. All Anaplasma sequences (N = 139) were characterized as A. ovis. For Bartonella species characterization, phylogenetic analyses of gltA and rpoB genes identified three Bartonella species including B. bovis, B. melophagi and Candidatus B. ovis. Bartonella bovis was detected in all 22-positive cattle, while B. melophagi and Candidatus B. ovis were found in four and three sheep, respectively. This study identifies a high prevalence of tick-borne pathogens within the livestock population and to our knowledge, is the first time NGS methods have been used to explore tick-borne diseases in Mongolia. Further research is needed in Mongolia to better understand the clinical and economic burdens associated with tick-borne diseases in both livestock and pastoral herder populations.
英文关键词	Tick-borne disease Next generation sequencing Mongolia Anaplasma Bartonella One health
类型	Article
语种	英语
开放获取类型	hybrid
收录类别	SCI-E
WOS记录号	WOS:000716994400002
WOS关键词	BARTONELLA-MELOPHAGI ; GENETIC-CHARACTERIZATION ; SMALL RUMINANTS ; ANAPLASMA-SPP. ; SHEEP KEDS ; OVIS ; INFECTION ; EHRLICHIA ; HENSELAE ; OVINUS
WOS类目	Infectious Diseases ; Microbiology ; Parasitology
WOS研究方向	Infectious Diseases ; Microbiology ; Parasitology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/374450
作者单位	[Chaorattanakawee, Suwanna; Takhampunya, Ratree; Poole-Smith, B. Katherine; Davidson, Silas] Armed Forces Res Inst Med Sci USAMD AFRIMS, Dept Entomol, US Army Med Directorate, Bangkok, Thailand; [Chaorattanakawee, Suwanna] Mahidol Univ, Fac Publ Hlth, Dept Parasitol & Entomol, Bangkok, Thailand; [Wofford, Rachel N.; Musih, Elisha; von Fricken, Michael E.] George Mason Univ, Dept Global & Community Hlth, 4400 Univ Dr, Fairfax, VA 22030 USA; [Boldbaatar, Bazartseren; Lkhagvatseren, Sukhbaatar] Mongolian Univ Life Sci, Ulaanbaatar, Mongolia; [Altantogtokh, Doniddemberel] Natl Ctr Zoonot Dis, Ulaanbaatar, Mongolia; [Nymadawa, Pagbajab] Mongolian Acad Med Sci, Ulaanbaatar, Mongolia; [Davidson, Silas] US Mil Acad, Dept Chem & Life Sci, West Point, NY 10996 USA; [Hertz, Jeffrey; Fiorenzano, Jodi] Naval Med Res Unit TWO NAMRU 2, Singapore, Singapore; [Gray, Gregory C.] Duke Univ, Div Infect Dis, Durham, NC USA; [Gray, Gregory C.] Duke Univ, Duke Global Hlth Inst, Durham, NC USA
推荐引用方式 GB/T 7714	Chaorattanakawee, Suwanna,Wofford, Rachel N.,Takhampunya, Ratree,et al. Tracking tick-borne diseases in Mongolian livestock using next generation sequencing (NGS)[J],2022,13(1).
APA	Chaorattanakawee, Suwanna.,Wofford, Rachel N..,Takhampunya, Ratree.,Poole-Smith, B. Katherine.,Boldbaatar, Bazartseren.,...&von Fricken, Michael E..(2022).Tracking tick-borne diseases in Mongolian livestock using next generation sequencing (NGS).TICKS AND TICK-BORNE DISEASES,13(1).
MLA	Chaorattanakawee, Suwanna,et al."Tracking tick-borne diseases in Mongolian livestock using next generation sequencing (NGS)".TICKS AND TICK-BORNE DISEASES 13.1(2022).