干旱区生态环境知识资源中心(Arid): iN siLico CHARACTERIZATION AND GENE EXPRESSION ANALYSIS OF LATE-EMBRYOGENESIS ABUNDANT PROTEINS OF AGAVE TEQUiLANA WEBER VAR. AZUL

Arid

DOI	10.17129/botsci.2861
	iN siLico CHARACTERIZATION AND GENE EXPRESSION ANALYSIS OF LATE-EMBRYOGENESIS ABUNDANT PROTEINS OF AGAVE TEQUiLANA WEBER VAR. AZUL
	Villegas-Camas, Jorge; Verdel-Aranda, Karina; Lara-Reyna, Joel; Martinez-Hernandez, Aida
通讯作者	Martinez-Hernandez, A (corresponding author), Colegio Postgrad, Campus Campeche, Champoton, Campeche, Mexico.
来源期刊	BOTANICAL SCIENCES
ISSN	2007-4298
EISSN	2007-4476
出版年	2022
卷号	100 期号:1 页码:169-191
英文摘要	Background/ Studied species: Agave tequilana Weber var. azul is an important crop in Mexico, used for tequila production. Many species of Agave grow in arid lands and are highly tolerant to those environmental conditions. However, the molecular bases of mechanisms selected in agaves to face abiotic stress, have not been described. Hypotheses: Late-embryogenesis abundant proteins (LEAPs), a superfamily closely associated to abiotic stress responses in plants, are a key element in agave responses to arid environments. Methods: Transcriptomic data from A. tequilana were used to perform in silico analyses to identify genes encoding Agave LEAPs. We compare their structural characteristics and their similarity/divergence with described LEAPs from other plant species using bioinformatics. The abun-dance of the AteqLEAP transcripts in vegetative organs and in response to high temperatures was determined by qRT-PCR. Results: We identified three structurally different AteqLEAPs. AteqLEA_5Bs show low similarity to LEAPs known as atypical (containing LEA_3 domain); and they exhibit an unexpected high and constitutive expression pattern in leaves. But AteqLEA_5C (LEA_2) transcripts are low-expressed in all the organs analyzed. Two isoforms of AteqDHN type SK3 show the typical structural disorder and hydrophilicity of dehy-drins and are highly expressed in undeveloped leaves, vegetative meristem and stem. Conclusions: AteqLEAP_5Bs seem to play a major and preventive protective role in fully photosynthetic leaves as in a primed state; whereas AteqDHNs presumably play a role protecting tissues in differentiation stage as meristem and developing leaves, and storage tissues such as the agave stem.
英文关键词	AgaveLEAP dehydrins gene expression pattern in silico structural analysis
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000726409400002
WOS关键词	RIBONUCLEIC-ACID POPULATIONS ; HYDROPHOBIC LEA PROTEIN ; ARABIDOPSIS-THALIANA ; ABSCISIC-ACID ; FUNCTIONAL-CHARACTERIZATION ; DEVELOPMENTAL BIOCHEMISTRY ; MOLECULAR CHARACTERIZATION ; COTTONSEED EMBRYOGENESIS ; HETEROLOGOUS EXPRESSION ; LOW-TEMPERATURE
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/374408
作者单位	[Villegas-Camas, Jorge; Lara-Reyna, Joel; Martinez-Hernandez, Aida] Colegio Postgrad, Campus Campeche, Champoton, Campeche, Mexico; [Verdel-Aranda, Karina] Tecnol Nacl Mexico, Inst Tecnol China, Campeche, Campeche, Mexico
推荐引用方式 GB/T 7714	Villegas-Camas, Jorge,Verdel-Aranda, Karina,Lara-Reyna, Joel,et al. iN siLico CHARACTERIZATION AND GENE EXPRESSION ANALYSIS OF LATE-EMBRYOGENESIS ABUNDANT PROTEINS OF AGAVE TEQUiLANA WEBER VAR. AZUL[J],2022,100(1):169-191.
APA	Villegas-Camas, Jorge,Verdel-Aranda, Karina,Lara-Reyna, Joel,&Martinez-Hernandez, Aida.(2022).iN siLico CHARACTERIZATION AND GENE EXPRESSION ANALYSIS OF LATE-EMBRYOGENESIS ABUNDANT PROTEINS OF AGAVE TEQUiLANA WEBER VAR. AZUL.BOTANICAL SCIENCES,100(1),169-191.
MLA	Villegas-Camas, Jorge,et al."iN siLico CHARACTERIZATION AND GENE EXPRESSION ANALYSIS OF LATE-EMBRYOGENESIS ABUNDANT PROTEINS OF AGAVE TEQUiLANA WEBER VAR. AZUL".BOTANICAL SCIENCES 100.1(2022):169-191.