干旱区生态环境知识资源中心(Arid): Biosurfactant production using Egyptian oil fields indigenous bacteria for microbial enhanced oil recovery

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DOI	10.1016/j.petrol.2021.109601
	Biosurfactant production using Egyptian oil fields indigenous bacteria for microbial enhanced oil recovery
	Aboelkhair, Hamed; Diaz, Pedro; Attia, Attia
通讯作者	Aboelkhair, H (corresponding author), British Univ Egypt, Fac Energy & Environm Engn, Dept Petr & Gas Technol, Misr-Ismalia Rd,El Shorouk City, Cairo 11837, Egypt.
来源期刊	JOURNAL OF PETROLEUM SCIENCE AND ENGINEERING
ISSN	0920-4105
EISSN	1873-4715
出版年	2022
卷号	208
英文摘要	Biosurfactant production is one of the most efficient mechanisms in microbial enhanced oil recovery (MEOR) processes. This work investigates the production of biosurfactants by indigenous bacteria isolated from Egyptian oil fields, and how to optimize these produced biosurfactants for MEOR. 59 Egyptian oil reservoirs were screened to evaluate the potential applicability of MEOR processes, based on their rock and fluid properties. Results showed that 8 reservoirs from the Gulf of Suez and 3 reservoirs from the Western Desert had the potential to MEOR. Combined analysis of morphological, and biochemical characterization was performed on the 11 bacterial strains isolated from different crude oil samples collected from the reservoirs that have the potential to MEOR process to identify their types. Bacillus spp, a bacilli species that can produce biosurfactants, was selected for further studies. To optimize the surface activity of the produced biosurfactant, ten different reported nutrient media, and a new proposed nutrient media were examined. Bacillus spp has shown the ability to produce a very active biosurfactant that reduced the surface tension of water from 71.8 +/- 1.9 mN/m to 25.7 +/- 1.2 mN/m, and the interfacial tension of water against kerosene from 48.4 +/- 2.1 mN/m to 0.38 +/- 0.07 mN/m at Critical Micelle Concentration (CMC) of 0.04 +/- 0.01 g/l, in a medium supplemented by the new proposed nutrient medium H. The growth rate of Bacillus spp was studied, and it was found it reached its maximum (OD600nm 2.59 +/- 0.16) after 24 h of incubation. Biosurfactant production has no significant change in its surface activity over a wide range of temperature range up to 120 degrees C, which means the studied species Bacillus spp is a thermophilic bacterium. Bacillus spp grew well in the presence of high salt concentration up to 20% (w/v) NaCl, the optimal surface activity was obtained in the range of 0-2% (w/v) NaCl, and at pH 7. The emulsification activity of the produced biosurfactant was examined, and it reached the maximum (69.6 +/- 1.5%) against kerosene at temperature 25 degrees C, Salinity 0% (w/v) NaCl (distilled water), and pH 7. The produced biosurfactant was purified and extracted by acid precipitation method, and the biosurfactant yield of the purified compound was found to be 2.8 +/- 0.3 g/l. Finally, the core-flooding experiments were conducted to investigate the effect of produced biosurfactants by Bacillus subtilis in oil recovery. The obtained results reveal the potential of Bacillus spp to grow in the new proposed medium H and produce effective and efficient biosurfactants that enhanced oil recovery by 25.19-39.35% of additional oil over the water flooding residual oil saturation in the studied cores and retain more than 60% of its surface activity under harsh conditions and that are relevant to Microbial Enhanced Oil Recovery, MEOR.
英文关键词	Microbial enhanced oil recovery Indigenous bacteria Biosurfactant Bacillus subtilis Surface activity Critical micelle concentration
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000727906800005
WOS关键词	BACILLUS-SUBTILIS ; SURFACTANT ; LICHENIFORMIS ; OPTIMIZATION ; WETTABILITY ; HYDROCARBON ; INJECTION ; MOLASSES ; STRAINS
WOS类目	Energy & Fuels ; Engineering, Petroleum
WOS研究方向	Energy & Fuels ; Engineering
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/374379
作者单位	[Aboelkhair, Hamed; Attia, Attia] British Univ Egypt, Fac Energy & Environm Engn, Dept Petr & Gas Technol, Misr-Ismalia Rd,El Shorouk City, Cairo 11837, Egypt; [Aboelkhair, Hamed; Diaz, Pedro] London S Bank Univ, Sch Engn, Div Chem & Energy Engn, 103 Borough Rd, London SE1 0AA, United Kingdom; [Aboelkhair, Hamed] British Univ Egypt, Fac Energy & Environm Engn, Dept Petr & Gas Technol, Misr-Ismalia Rd,El Shorouk City, Cairo 11837, Egypt
推荐引用方式 GB/T 7714	Aboelkhair, Hamed,Diaz, Pedro,Attia, Attia. Biosurfactant production using Egyptian oil fields indigenous bacteria for microbial enhanced oil recovery[J],2022,208.
APA	Aboelkhair, Hamed,Diaz, Pedro,&Attia, Attia.(2022).Biosurfactant production using Egyptian oil fields indigenous bacteria for microbial enhanced oil recovery.JOURNAL OF PETROLEUM SCIENCE AND ENGINEERING,208.
MLA	Aboelkhair, Hamed,et al."Biosurfactant production using Egyptian oil fields indigenous bacteria for microbial enhanced oil recovery".JOURNAL OF PETROLEUM SCIENCE AND ENGINEERING 208(2022).