干旱区生态环境知识资源中心(Arid): Simultaneous Quantitative Screening for Pain Medications in Serum by High-Performance Liquid Chromatography/Time-of-Flight Mass Spectrometry with Solid-Phase Dispersive Extraction

Arid

DOI	10.15583/jpchrom.2021.001
	Simultaneous Quantitative Screening for Pain Medications in Serum by High-Performance Liquid Chromatography/Time-of-Flight Mass Spectrometry with Solid-Phase Dispersive Extraction
	Saito, Koichi; Nishiyama, Rika; Ito, Rie
通讯作者	Saito, K (corresponding author), Hoshi Univ, Fac Pharmaceut Sci, Dept Analyt Chem, Shinagawa Ku, 2-4-41 Ebara, Tokyo 1428501, Japan.
来源期刊	CHROMATOGRAPHY
ISSN	1342-8284
EISSN	1348-3315
出版年	2021
卷号	42 期号:2 页码:83-90
英文摘要	In this study, solid-phase dispersive extraction (SPDE) was used for serum pretreatment and in the simultaneous analysis of analgesics and adjuvant analgesics (30 types in total) that are usually used as first-and second-choice treatments for pain patients, by liquid chromatography/time-of-flight mass spectrometry (LC/TOF-MS). Examination of the optimum conditions for SPDE using Oasis MCX as the solid-phase gel revealed that the recovery rates for serum samples deproteinized in advance were 49-87%, whereas the recovery rates were as high as 78-112% when deproteinization was not performed. The matrix effect was within +/- 10% regardless of the presence or absence of deproteinization, and its influence could be suppressed even if deproteinization was not performed. The results indicate that serum deproteinization was unnecessary when SPDE was used for pretreatment. In LC/TOF-MS measurement, gradient elution was carried out using core-shell type column Kinetex C18 (150 mm x 2.1 mm, 1.7 mu m) as the LC column and 50 mM ammonium acetate buffer (pH 7.8)/acetonitrile/methanol mixture as the mobile phase. The 30 drugs were well separated, and the limit of quantification was 0.25-10 ng/mL, the correlation coefficients of the calibration curves were higher than 0.998, and the average recoveries ranged from 77.7 to 112.1%. The method would be useful to screen for analgesics and adjuvant analgesics (30 types in total) in serum in the fields of forensic science and emergency medicine.
英文关键词	Pain medication Solid-phase dispersive extraction LC/TOF-MS Serum
类型	Article
语种	英语
开放获取类型	gold
收录类别	ESCI
WOS记录号	WOS:000718778300002
WOS关键词	NEUROPATHIC PAIN ; ANTIDEPRESSANTS ; METABOLITES ; PLASMA ; DRUGS
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/374249
作者单位	[Saito, Koichi; Nishiyama, Rika; Ito, Rie] Hoshi Univ, Fac Pharmaceut Sci, Dept Analyt Chem, Shinagawa Ku, 2-4-41 Ebara, Tokyo 1428501, Japan
推荐引用方式 GB/T 7714	Saito, Koichi,Nishiyama, Rika,Ito, Rie. Simultaneous Quantitative Screening for Pain Medications in Serum by High-Performance Liquid Chromatography/Time-of-Flight Mass Spectrometry with Solid-Phase Dispersive Extraction[J],2021,42(2):83-90.
APA	Saito, Koichi,Nishiyama, Rika,&Ito, Rie.(2021).Simultaneous Quantitative Screening for Pain Medications in Serum by High-Performance Liquid Chromatography/Time-of-Flight Mass Spectrometry with Solid-Phase Dispersive Extraction.CHROMATOGRAPHY,42(2),83-90.
MLA	Saito, Koichi,et al."Simultaneous Quantitative Screening for Pain Medications in Serum by High-Performance Liquid Chromatography/Time-of-Flight Mass Spectrometry with Solid-Phase Dispersive Extraction".CHROMATOGRAPHY 42.2(2021):83-90.