干旱区生态环境知识资源中心(Arid): Post-release immune responses of Tasmanian devils vaccinated with an experimental devil facial tumour disease vaccine

Arid

DOI	10.1071/WR20210
	Post-release immune responses of Tasmanian devils vaccinated with an experimental devil facial tumour disease vaccine
	Pye, Ruth; Darby, Jocelyn; Flies, Andrew S.; Fox, Samantha; Carver, Scott; Elmer, Jodie; Swift, Kate; Hogg, Carolyn; Pemberton, David; Woods, Gregory; Lyons, A. Bruce
通讯作者	Pye, R (corresponding author), Univ Tasmania, Menzies Inst Med Res, 17 Liverpool St, Hobart, Tas 7000, Australia. ; Lyons, AB (corresponding author), Univ Tasmania, Coll Hlth & Med, Tasmanian Sch Med, 17 Liverpool St, Hobart, Tas 7000, Australia.
来源期刊	WILDLIFE RESEARCH
ISSN	1035-3712
EISSN	1448-5494
出版年	2021
卷号	48 期号:8 页码:701-712
英文摘要	Context. Disease is increasingly becoming a driver of wildlife population declines and an extinction risk. Vaccines are one of the most successful health interventions in human history, but few have been tested for mitigating wildlife disease. The transmissible cancer, devil facial tumour disease (DFTD), triggered the Tasmanian devil's (Sarcophilus harrisii) inclusion on the international endangered species list. In 2016, 33 devils from a DFTD-free insurance population were given an experimental DFTD vaccination before their wild release on the Tasmanian northern coast. Aim. To determine the efficacy of the vaccination protocol and the longevity of the induced responses. Method. Six trapping trips took place over the 2.5 years following release, and both vaccinated and incumbent devils had blood samples and tumour biopsies collected. Key results. In all, 8 of the 33 vaccinated devils were re-trapped, and six of those developed DFTD within the monitoring period. Despite the lack of protection provided by the vaccine, we observed signs of immune activation not usually found in unvaccinated devils. First, sera collected from the eight devils showed that anti-DFTD antibodies persisted for up to 2 years post-vaccination. Second, tumour-infiltrating lymphocytes were found in three of four biopsies collected from vaccinated devils, which contrasts with the 'immune deserts' typical of DFTs; only 1 of the 20 incumbent devils with DFTD had a tumour biopsy exhibiting immune-cell infiltrate. Third, immunohistochemical analysis of the vaccinated devils' tumour biopsies identified the functional immune molecules associated with antigen-presenting cells (MHC-II) and T-cells (CD3), and the immune checkpoint molecule PD-1, all being associated with anti-tumour immunity in other species. Conclusions. These results correlate with our previous study on captive devils in which a prophylactic vaccine primed the devil immune system and, following DFTD challenge and tumour growth, immunotherapy induced complete tumour regressions. The field trial results presented here provide further evidence that the devil immune system can be primed to recognise DFTD cells, but additional immune manipulation could be needed for complete protection or induction of tumour regressions.
英文关键词	devil facial tumour disease transmissible cancer vaccine immunohistochemistry
类型	Article
语种	英语
开放获取类型	Green Accepted, Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000728194400001
WOS关键词	TRANSMISSIBLE CANCERS ; POPULATION ; SURVIVAL ; CELLS
WOS类目	Ecology ; Zoology
WOS研究方向	Environmental Sciences & Ecology ; Zoology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/374122
作者单位	[Pye, Ruth; Darby, Jocelyn; Flies, Andrew S.; Woods, Gregory] Univ Tasmania, Menzies Inst Med Res, 17 Liverpool St, Hobart, Tas 7000, Australia; [Fox, Samantha; Elmer, Jodie; Swift, Kate; Pemberton, David] Dept Primary Ind Pk Water & Environm, 134 Macquarie St, Hobart, Tas 7000, Australia; [Carver, Scott] Univ Tasmania, Dept Biol Sci, Private Bag 51, Hobart, Tas 7001, Australia; [Hogg, Carolyn] Univ Sydney, Sch Life & Environm Sci, Australasian Wildlife Genom Grp, Camperdown, NSW 2050, Australia; [Lyons, A. Bruce] Univ Tasmania, Coll Hlth & Med, Tasmanian Sch Med, 17 Liverpool St, Hobart, Tas 7000, Australia; [Fox, Samantha] Toledo Zoo, 2605 Broadway, Toledo, OH 43609 USA
推荐引用方式 GB/T 7714	Pye, Ruth,Darby, Jocelyn,Flies, Andrew S.,et al. Post-release immune responses of Tasmanian devils vaccinated with an experimental devil facial tumour disease vaccine[J],2021,48(8):701-712.
APA	Pye, Ruth.,Darby, Jocelyn.,Flies, Andrew S..,Fox, Samantha.,Carver, Scott.,...&Lyons, A. Bruce.(2021).Post-release immune responses of Tasmanian devils vaccinated with an experimental devil facial tumour disease vaccine.WILDLIFE RESEARCH,48(8),701-712.
MLA	Pye, Ruth,et al."Post-release immune responses of Tasmanian devils vaccinated with an experimental devil facial tumour disease vaccine".WILDLIFE RESEARCH 48.8(2021):701-712.