干旱区生态环境知识资源中心(Arid): RNAi of vacuolar-type H+-ATPase genes causes growth delay and molting defect in Henosepilachna vigintioctopunctata

Arid

DOI	10.1017/S0007485321000420
	RNAi of vacuolar-type H+-ATPase genes causes growth delay and molting defect in Henosepilachna vigintioctopunctata
	Zeng, Jie; Mu, Li-Li; Jin, Lin; Ali Anjum, Ahmad; Li, Guo-Qing
通讯作者	Li, GQ (corresponding author), Nanjing Agr Univ, Coll Plant Protect, Educ Minist Key Lab Integrated Management Crop Di, Nanjing 210095, Peoples R China.
来源期刊	BULLETIN OF ENTOMOLOGICAL RESEARCH
ISSN	0007-4853
EISSN	1475-2670
出版年	2021
卷号	111 期号:6 页码:705-714
英文摘要	Henosepilachna vigintioctopunctata is one of the most serious insect pests to a large number of nightshades and cucurbits. RNA interference (RNAi) triggered by double-stranded RNA (dsRNA) offers a reduced risk approach to control the beetle. Identification of amenable target genes and determination of appropriate life stage for dsRNA treatment are two critical steps in order to improve RNAi efficiency. In the present paper, we identified three vATPase genes, namely HvvATPaseC, HvvATPaseE and HvvATPaseH. We found that the three transcripts were widely expressed in the eggs, first- to fourth-instar larvae, prepupae, pupae and adults. They were abundantly transcribed in the hindgut and Malpighian tubules, in contrast to the epidermis and fat body. Three days' ingestion of dsvATPaseC, dsvATPaseE and dsvATPaseH by the fourth-instar larvae significantly decreased corresponding transcript level by 90.1, 88.9 and 97.2%, greatly reduced larval fresh weight by 28.0, 29.9 and 28.0%, and caused 66.7, 100 and 78.7% larval lethality respectively. Comparably, 3 days' exposure of the third-instar larvae to dsvATPaseC significantly reduced HvvATPaseC mRNA level by 89.5%, decreased approximately 80% of the larval fresh weight, and killed 100% of the treated larvae. Therefore, the three vATPase genes, especially HvvATPaseE, are potential amenable target genes and young larvae are more susceptible to dsRNA. Our findings will enable the development of the dsRNA-based pesticide to control H. vigintioctopunctata.
英文关键词	Henosepilachna vigintioctopunctata larval growth molting defect RNA interference vacuolar-type H+-ATPase
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000721300100009
WOS关键词	DOUBLE-STRANDED-RNA ; COLORADO POTATO BEETLE ; LEPTINOTARSA-DECEMLINEATA ; V-ATPASE ; MANDUCA-SEXTA ; DSRNA DEGRADATION ; DESERT LOCUST ; SUBUNIT-C ; INTERFERENCE ; IDENTIFICATION
WOS类目	Entomology
WOS研究方向	Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/374074
作者单位	[Zeng, Jie; Mu, Li-Li; Jin, Lin; Ali Anjum, Ahmad; Li, Guo-Qing] Nanjing Agr Univ, Coll Plant Protect, Educ Minist Key Lab Integrated Management Crop Di, Nanjing 210095, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Jie,Mu, Li-Li,Jin, Lin,et al. RNAi of vacuolar-type H+-ATPase genes causes growth delay and molting defect in Henosepilachna vigintioctopunctata[J],2021,111(6):705-714.
APA	Zeng, Jie,Mu, Li-Li,Jin, Lin,Ali Anjum, Ahmad,&Li, Guo-Qing.(2021).RNAi of vacuolar-type H+-ATPase genes causes growth delay and molting defect in Henosepilachna vigintioctopunctata.BULLETIN OF ENTOMOLOGICAL RESEARCH,111(6),705-714.
MLA	Zeng, Jie,et al."RNAi of vacuolar-type H+-ATPase genes causes growth delay and molting defect in Henosepilachna vigintioctopunctata".BULLETIN OF ENTOMOLOGICAL RESEARCH 111.6(2021):705-714.