干旱区生态环境知识资源中心(Arid): De novo transcriptome sequencing, assembly, and gene expression profiling of a salt-stressed halophyte (Salsola drummondii) from a saline habitat

Arid

DOI	10.1111/ppl.13591
	De novo transcriptome sequencing, assembly, and gene expression profiling of a salt-stressed halophyte (Salsola drummondii) from a saline habitat
	Elnaggar, Attiat; Mosa, Kareem A.; Ramamoorthy, Kalidoss; El-Keblawy, Ali; Navarro, Teresa; Soliman, Sameh S. M.
通讯作者	Mosa, KA (corresponding author), Univ Sharjah, Dept Appl Biol, Coll Sci, POB 27272, Sharjah, U Arab Emirates.
来源期刊	PHYSIOLOGIA PLANTARUM
ISSN	0031-9317
EISSN	1399-3054
出版年	2021
卷号	173 期号:4 页码:1695-1714
英文摘要	Salsola drummondii is a perennial habitat-indifferent halophyte growing in saline and nonsaline habitats of the Arabian hyperarid deserts. It offers an invaluable opportunity to examine the molecular mechanisms of salt tolerance. The present study was conducted to elucidate these mechanisms through transcriptome profiling of seedlings grown from seeds collected in a saline habitat. The Illumina Hiseq 2500 platform was employed to sequence cDNA libraries prepared from shoots and roots of nonsaline-treated plants (controls) and plants treated with 1200mM NaCl. Transcriptomic comparison between salt-treated and control samples resulted in 17,363 differentially expressed genes (DEGs), including 12,000 upregulated genes (7870 in roots, 4130 in shoots) and 5363 downregulated genes (4258 in roots and 1105 in shoots). The majority of identified DEGs are known to be involved in transcription regulation (79), signal transduction (82), defense metabolism (101), transportation (410), cell wall metabolism (27), regulatory processes (392), respiration (85), chaperoning (9), and ubiquitination (98) during salt tolerance. This study identified potential genes associated with the salt tolerance of S. drummondii and demonstrated that this tolerance may depend on the induction of certain genes in shoot and root tissues. These gene expressions were validated using reverse-transcription quantitative PCR, the results of which were consistent with transcriptomics results. To the best of our knowledge, this is the first study providing genetic information on salt tolerance mechanisms in S. drummondii.
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000723341000028
WOS关键词	ABA SIGNAL-TRANSDUCTION ; PROTEOMIC ANALYSIS ; ABIOTIC STRESS ; ABSCISIC-ACID ; TOLERANCE GENES ; ARABIDOPSIS-THALIANA ; GIBBERELLIC-ACID ; PROTEIN ; RESPONSES ; MECHANISMS
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/374042
作者单位	[Elnaggar, Attiat; Mosa, Kareem A.; Ramamoorthy, Kalidoss; El-Keblawy, Ali] Univ Sharjah, Dept Appl Biol, Coll Sci, POB 27272, Sharjah, U Arab Emirates; [Elnaggar, Attiat] Alexandria Univ, Dept Bot & Microbiol, Fac Sci, Alexandria, Egypt; [Elnaggar, Attiat; Navarro, Teresa] Univ Malaga, Dept Botan Fisiol Vegetal, Malaga, Spain; [Mosa, Kareem A.] Al Azhar Univ, Dept Biotechnol, Fac Agr, Cairo, Egypt; [El-Keblawy, Ali] Al Arish Univ, Dept Biol, Fac Sci, Cairo, Egypt; [Soliman, Sameh S. M.] Univ Sharjah, Dept Med Chem, Coll Pharm, Sharjah, U Arab Emirates
推荐引用方式 GB/T 7714	Elnaggar, Attiat,Mosa, Kareem A.,Ramamoorthy, Kalidoss,et al. De novo transcriptome sequencing, assembly, and gene expression profiling of a salt-stressed halophyte (Salsola drummondii) from a saline habitat[J],2021,173(4):1695-1714.
APA	Elnaggar, Attiat,Mosa, Kareem A.,Ramamoorthy, Kalidoss,El-Keblawy, Ali,Navarro, Teresa,&Soliman, Sameh S. M..(2021).De novo transcriptome sequencing, assembly, and gene expression profiling of a salt-stressed halophyte (Salsola drummondii) from a saline habitat.PHYSIOLOGIA PLANTARUM,173(4),1695-1714.
MLA	Elnaggar, Attiat,et al."De novo transcriptome sequencing, assembly, and gene expression profiling of a salt-stressed halophyte (Salsola drummondii) from a saline habitat".PHYSIOLOGIA PLANTARUM 173.4(2021):1695-1714.