干旱区生态环境知识资源中心(Arid): Species identification and phylogenetic analysis of Leishmania isolated from patients, vectors and hares in the Xinjiang Autonomous Region, The People's Republic of China

Arid

DOI	10.1371/journal.pntd.0010055
	Species identification and phylogenetic analysis of Leishmania isolated from patients, vectors and hares in the Xinjiang Autonomous Region, The People's Republic of China
	Chen, Yun-Fu; Liao, Li-Fu; Wu, Na; Gao, Jiang-Mei; Zhang, Peng; Wen, Yan-Zi; Hide, Geoff; Lai, De-Hua; Lun, Zhao-Rong
通讯作者	Lai, DH ; Lun, ZR (corresponding author), Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Key Lab Gene Engn,Minist Educ, Guangzhou, Peoples R China. ; Lun, ZR (corresponding author), Univ Salford, Ecosyst & Environm Res Ctr, Salford, Lancs, England. ; Lun, ZR (corresponding author), Univ Salford, Biomed Res Ctr, Sch Sci Engn & Environm, Salford, Lancs, England.
来源期刊	PLOS NEGLECTED TROPICAL DISEASES
ISSN	1935-2735
出版年	2021
卷号	15 期号:12
英文摘要	BackgroundVisceral leishmaniasis (VL) has been declared as one of the six major tropical diseases by the World Health Organization. This disease has been successfully controlled in China, except for some areas in the western region, such as the Xinjiang Autonomous Region, where both anthroponotic VL (AVL) and desert type zoonotic VL (DT-ZVL) remain endemic with sporadic epidemics. Methodology/Principal findingsHere, an eleven-year survey (2004-2014) of Leishmania species, encompassing both VL types isolated from patients, sand-fly vectors and Tarim hares (Lepus yarkandensis) from the Xinjiang Autonomous Region was conducted, with a special emphasis on the hares as a potential reservoir animal for DT-ZVL. Key diagnostic genes, ITS1, hsp70 and nagt (encoding N-acetylglucosamine-1-phosphate transferase) were used for phylogenetic analyses, placing all Xinjiang isolates into one clade of the L. donovani complex. Unexpectedly, AVL isolates were found to be closely related to L. infantum, while DT-ZVL isolates were closer to L. donovani. Unrooted parsimony networks of haplotypes for these isolates also revealed their relationship. Conclusions/SignificanceThe above analyses of the DT-ZVL isolates suggested their geographic isolation and independent evolution. The sequence identity of isolates from patients, vectors and the Tarim hares in a single DT-ZVL site provides strong evidence in support of this species as an animal reservoir. Author summaryBlack faver, also known as visceral leishmaniasis (VL), is caused by pathogens of Leishmania species, spread by the bites of infected sand flies. This disease has been successfully controlled in China, except for some areas in the western region, such as Xinjiang. However, the knowledge on Leishmania in these areas remains a few important gaps. Particularly, what is the animal reservoir for desert type zoonotic VL (DT-ZVL), as sand flies get infected in areas free of patient or infected dogs? To address this question, an eleven-year survey (2004-2014) in Xinjiang for Leishmania species was carried out. We found that VLs in Xinjiang are contributed to Leishmania donovani complex, and Tarim hares is likely the reservoir animal for DT-ZVL.
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000731451900003
WOS关键词	VISCERAL LEISHMANIASIS ; INFANTUM ; DIVERSITY ; OUTBREAK
WOS类目	Infectious Diseases ; Parasitology ; Tropical Medicine
WOS研究方向	Infectious Diseases ; Parasitology ; Tropical Medicine
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/373965
作者单位	[Chen, Yun-Fu; Wu, Na; Lai, De-Hua; Lun, Zhao-Rong] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Key Lab Gene Engn,Minist Educ, Guangzhou, Peoples R China; [Liao, Li-Fu] Ctr Lab Anim Res, Xinjiang Uighur Autonomous Reg Ctr Dis Control, Urumqi, Peoples R China; [Gao, Jiang-Mei] Guangdong Acad Sci, Inst zool, Guangdong Key Lab Anim Conservat & Resource Utili, Guangzhou, Peoples R China; [Hide, Geoff; Lun, Zhao-Rong] Univ Salford, Ecosyst & Environm Res Ctr, Salford, Lancs, England; [Hide, Geoff; Lun, Zhao-Rong] Univ Salford, Biomed Res Ctr, Sch Sci Engn & Environm, Salford, Lancs, England
推荐引用方式 GB/T 7714	Chen, Yun-Fu,Liao, Li-Fu,Wu, Na,et al. Species identification and phylogenetic analysis of Leishmania isolated from patients, vectors and hares in the Xinjiang Autonomous Region, The People's Republic of China[J],2021,15(12).
APA	Chen, Yun-Fu.,Liao, Li-Fu.,Wu, Na.,Gao, Jiang-Mei.,Zhang, Peng.,...&Lun, Zhao-Rong.(2021).Species identification and phylogenetic analysis of Leishmania isolated from patients, vectors and hares in the Xinjiang Autonomous Region, The People's Republic of China.PLOS NEGLECTED TROPICAL DISEASES,15(12).
MLA	Chen, Yun-Fu,et al."Species identification and phylogenetic analysis of Leishmania isolated from patients, vectors and hares in the Xinjiang Autonomous Region, The People's Republic of China".PLOS NEGLECTED TROPICAL DISEASES 15.12(2021).