干旱区生态环境知识资源中心(Arid): Properties of Modestobacter deserti sp. nov., a Kind of Novel Phosphate-Solubilizing Actinobacteria Inhabited in the Desert Biological Soil Crusts

Arid

DOI	10.3389/fmicb.2021.742798
	Properties of Modestobacter deserti sp. nov., a Kind of Novel Phosphate-Solubilizing Actinobacteria Inhabited in the Desert Biological Soil Crusts
	Jiang, Zhu-Ming; Zhang, Bing-Huo; Sun, Hong-Min; Zhang, Tao; Yu, Li-Yan; Zhang, Yu-Qin
通讯作者	Zhang, YQ (corresponding author), Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biotechnol, Beijing, Peoples R China.
来源期刊	FRONTIERS IN MICROBIOLOGY
EISSN	1664-302X
出版年	2021
卷号	12
英文摘要	Three Gram-stain-positive, aerobic, motile actinobacterial strains designated as CPCC 205119(T), CPCC 205215, and CPCC 205251 were isolated from different biological soil crust samples collected from Tengger Desert, China. The 16S rRNA gene sequence comparison of these three strains showed they had almost identical 16S rRNA genes, which were closely related to members of the family Geodermatophilaceae, with the highest similarities of 96.3-97.3% to the species of Modestobacter. In the phylogenetic tree based on 16S rRNA gene sequences, these isolates clustered into a subclade next to the branch containing the species of Modestobacter lapidis and Modestobacter multiseptatus, within the lineage of the genus Modestobacter. The comparative genomic characteristics (values of ANI, dDDH, AAI, and POCP) and the phenotypic properties (morphological, physiological, and chemotaxonomic characteristics) of these isolates readily supported to affiliate them to the genus Modestobacter as a single separate species. For which, we proposed that the isolates CPCC 205119(T), CPCC 205215, and CPCC 205251 represent a novel species of the genus Modestobacter as Modestobacter deserti sp. nov. CPCC 205119(T) (=I12A-02624=NBRC 113528(T)=KCTC 49201(T)) is the type strain. The genome of strain CPCC 205119(T) consisted of one chromosome (4,843,235bp) containing 4,424 coding genes, 48 tRNA genes, five rRNA genes, three other ncRNA genes, and 101 pseudogenes, with G+C content of 74.7%. The whole-genome sequences analysis indicated that this species contained alkaline phosphatase genes (phoA/phoD), phosphate transport-related genes (phoU, phnC, phnD, phnE, phoB, phoH, phoP, phoR, pitH, ppk, pstA, pstB, pstC, and pstS), trehalose-phosphate synthase gene (otsA), trehalose 6-phosphate phosphatase gene (otsB) and other encoding genes for the properties that help the microorganisms to adapt to harsh environmental conditions prevalent in deserts. Strains of this species could solubilize tricalcium phosphate [Ca-3(PO4)(2)] and phytin, assimilate pyrophosphate, thiophosphate, dithiophosphate, phosphoenol pyruvate, 2-deoxy-d-glucose-6-phosphate, and cysteamine-S-phosphate.
英文关键词	Modestobacter deserti average nucleotide identity pan-genome phenotype genotype biological soil crusts
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000720338900001
WOS关键词	GENOME SEQUENCE ; ALKALINE-PHOSPHATASE ; ESCHERICHIA-COLI ; GENE CLONING ; BINDING ; EXPRESSION ; CSRA ; IDENTIFICATION ; ACTINOMYCETE ; MECHANISMS
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/373839
作者单位	[Jiang, Zhu-Ming; Sun, Hong-Min; Zhang, Tao; Yu, Li-Yan; Zhang, Yu-Qin] Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biotechnol, Beijing, Peoples R China; [Zhang, Bing-Huo] Jiujiang Univ, Coll Life Sci, Jiujiang, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Zhu-Ming,Zhang, Bing-Huo,Sun, Hong-Min,et al. Properties of Modestobacter deserti sp. nov., a Kind of Novel Phosphate-Solubilizing Actinobacteria Inhabited in the Desert Biological Soil Crusts[J],2021,12.
APA	Jiang, Zhu-Ming,Zhang, Bing-Huo,Sun, Hong-Min,Zhang, Tao,Yu, Li-Yan,&Zhang, Yu-Qin.(2021).Properties of Modestobacter deserti sp. nov., a Kind of Novel Phosphate-Solubilizing Actinobacteria Inhabited in the Desert Biological Soil Crusts.FRONTIERS IN MICROBIOLOGY,12.
MLA	Jiang, Zhu-Ming,et al."Properties of Modestobacter deserti sp. nov., a Kind of Novel Phosphate-Solubilizing Actinobacteria Inhabited in the Desert Biological Soil Crusts".FRONTIERS IN MICROBIOLOGY 12(2021).