干旱区生态环境知识资源中心(Arid): Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata

Arid

DOI	10.1038/s41598-021-00833-2
	Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata
	Li, Rong; Cui, Kaiwen; Xie, Quanliang; Xie, Shuangquan; Chen, Xifeng; Zhuo, Lu; Cao, Aiping; Shen, Haitao; Jin, Xiang; Wang, Fei; Li, Hongbin
通讯作者	Wang, F ; Li, HB (corresponding author), Shihezi Univ, Coll Life Sci, Key Lab Oasis Town & Mt Basin Syst Ecol Xinjiang, Shihezi, Xinjiang, Peoples R China. ; Wang, F ; Li, HB (corresponding author), Shihezi Univ, Key Lab Xinjiang Phytomed Resource & Utilizat, Minist Educ, Shihezi, Xinjiang, Peoples R China.
来源期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
出版年	2021
卷号	11 期号:1
英文摘要	The desert pioneer plant Stipagrostis pennata plays an important role in sand fixation, wind prevention, and desert ecosystem recovery. An absence of reference genes greatly limits investigations into the regulatory mechanism by which S. pennata adapts to adverse desert environments at the molecular and genetic levels. In this study, eight candidate reference genes were identified from rhizosheath development transcriptome data from S. pennata, and their expression stability in the rhizosheaths at different development stages, in a variety of plant tissues, and under drought stress was evaluated using four procedures, including geNorm, NormFinder, BestKeeper, and RefFinder. The results showed that GAPDH and elF were the most stable reference genes under drought stress and in rhizosheath development, and ARP6 and ALDH were relatively stable in all plant tissues. In addition, elF was the most suitable reference gene for all treatments. Analysis of the consistency between the reverse transcription-quantitative PCR (RT-qPCR) and RNA sequencing data showed that the identified elF and GAPDH reference genes were stable during rhizosheath development. These results provide reliable reference genes for assuring the accuracy of RT-qPCR and offer a foundation for further investigations into the genetic responses of S. pennata to abiotic stress.
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000714977900042
WOS关键词	RELIABLE REFERENCE GENES ; HOUSEKEEPING GENES ; INTERNAL CONTROL ; PCR ANALYSIS ; VALIDATION ; NORMALIZATION ; RICE ; PAIR
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/373836
作者单位	[Li, Rong; Cui, Kaiwen; Xie, Quanliang; Xie, Shuangquan; Chen, Xifeng; Zhuo, Lu; Cao, Aiping; Shen, Haitao; Jin, Xiang; Wang, Fei; Li, Hongbin] Shihezi Univ, Coll Life Sci, Key Lab Oasis Town & Mt Basin Syst Ecol Xinjiang, Shihezi, Xinjiang, Peoples R China; [Li, Rong; Cui, Kaiwen; Xie, Quanliang; Xie, Shuangquan; Chen, Xifeng; Zhuo, Lu; Cao, Aiping; Shen, Haitao; Wang, Fei; Li, Hongbin] Shihezi Univ, Key Lab Xinjiang Phytomed Resource & Utilizat, Minist Educ, Shihezi, Xinjiang, Peoples R China; [Jin, Xiang] Hainan Normal Univ, Coll Life Sci, Key Lab Ecol Trop Isl, Minist Educ, Haikou, Hainan, Peoples R China
推荐引用方式 GB/T 7714	Li, Rong,Cui, Kaiwen,Xie, Quanliang,et al. Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata[J],2021,11(1).
APA	Li, Rong.,Cui, Kaiwen.,Xie, Quanliang.,Xie, Shuangquan.,Chen, Xifeng.,...&Li, Hongbin.(2021).Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata.SCIENTIFIC REPORTS,11(1).
MLA	Li, Rong,et al."Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata".SCIENTIFIC REPORTS 11.1(2021).