干旱区生态环境知识资源中心(Arid): Single-Cell Proteomic Analysis Dissects the Complexity of Tumor Microenvironment in Muscle Invasive Bladder Cancer

Arid

DOI	10.3390/cancers13215440
	Single-Cell Proteomic Analysis Dissects the Complexity of Tumor Microenvironment in Muscle Invasive Bladder Cancer
	Feng, Chao; Wang, Xi; Tao, Yuting; Xie, Yuanliang; Lai, Zhiyong; Li, Zhijian; Hu, Jiaxin; Tang, Shaomei; Pan, Lixin; He, Liangyu; Wang, Qiuyan; Li, Tianyu; Mo, Zengnan
通讯作者	Wang, QY ; Li, TY (corresponding author), Guangxi Med Univ, Ctr Genom & Personalized Med, Nanning 530021, Peoples R China. ; Wang, QY ; Li, TY (corresponding author), Guangxi Collaborat Innovat Ctr Genom & Personaliz, Guangxi Key Lab Genom & Personalized Med, Nanning 530021, Peoples R China. ; Wang, QY (corresponding author), Guangxi Med Univ, Ctr Translat Med, Chinese Minist Educ, Key Lab Longev & Aging Related Dis, Nanning 530021, Peoples R China. ; Li, TY (corresponding author), Guangxi Med Univ, Affiliated Hosp 1, Inst Urol & Nephrol, Nanning 530021, Peoples R China. ; Li, TY (corresponding author), Guangxi Med Univ, Affiliated Hosp 1, Dept Urol, Nanning 530021, Peoples R China.
来源期刊	CANCERS
EISSN	2072-6694
出版年	2021
卷号	13 期号:21
英文摘要	Simple Summary:& nbsp;The tumor microenvironment (TME) is considered to play a key role in the development of many types of tumors. Muscle invasive bladder cancer (MIBC), which is well known for its heterogeneity, has a highly complex TME. Herein, we integrated mass cytometry and imaging mass cytometry to systematically investigate the complexity of the MIBC TME. Our investigation revealed tumor and immune cells with diverse phenotypes. We identified a specific cancer stem-like cell cluster (ALDH(+)PD-L1(+)ER-beta(-)), which is associated with poor prognosis and highlighted the importance of the spatial distribution patterns of MIBC TME components. The present study comprehensively elucidated the complexity of the MIBC TME and provides potentially valuable information for future research. Muscle invasive bladder cancer (MIBC) is a malignancy with considerable heterogeneity. The MIBC tumor microenvironment (TME) is highly complex, comprising diverse phenotypes and spatial architectures. The complexity of the MIBC TME must be characterized to provide potential targets for precision therapy. Herein, an integrated combination of mass cytometry and imaging mass cytometry was used to analyze tumor cells, immune cells, and TME spatial characteristics of 44 MIBC patients. We detected tumor and immune cell clusters with abnormal phenotypes. In particular, we identified a previously overlooked cancer stem-like cell cluster (ALDH(+)PD-L1(+)ER-beta(-)) that was strongly associated with poor prognosis. We elucidated the different spatial architectures of immune cells (excluded, infiltrated, and deserted) and tumor-associated collagens (curved, stretched, directionally distributed, and chaotic) in the MIBC TME. The present study is the first to provide in-depth insight into the complexity of the MIBC TME at the single-cell level. Our results will improve the general understanding of the heterogeneous characteristics of MIBC, potentially facilitating patient stratification and personalized therapy.
英文关键词	muscle invasive bladder cancer tumor microenvironment cancer stem cell mass cytometry imaging mass cytometry
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000718630900001
WOS关键词	UROTHELIAL CARCINOMA ; PROGRESSION
WOS类目	Oncology
WOS研究方向	Oncology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/373817
作者单位	[Feng, Chao; Wang, Xi; Tao, Yuting; Xie, Yuanliang; Lai, Zhiyong; Li, Zhijian; Hu, Jiaxin; Tang, Shaomei; Pan, Lixin; He, Liangyu; Wang, Qiuyan; Li, Tianyu; Mo, Zengnan] Guangxi Med Univ, Ctr Genom & Personalized Med, Nanning 530021, Peoples R China; [Feng, Chao; Wang, Xi; Tao, Yuting; Xie, Yuanliang; Lai, Zhiyong; Li, Zhijian; Hu, Jiaxin; Tang, Shaomei; Pan, Lixin; He, Liangyu; Wang, Qiuyan; Li, Tianyu; Mo, Zengnan] Guangxi Collaborat Innovat Ctr Genom & Personaliz, Guangxi Key Lab Genom & Personalized Med, Nanning 530021, Peoples R China; [Feng, Chao; Tao, Yuting] Guangxi Med Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Nanning 530021, Peoples R China; [Wang, Xi; Wang, Qiuyan] Guangxi Med Univ, Ctr Translat Med, Chinese Minist Educ, Key Lab Longev & Aging Related Dis, Nanning 530021, Peoples R China; [Xie, Yuanliang] Guangxi Med Univ, Affiliated Tumor Hosp, Dept Urol, Nanning 530021, Peoples R China; [He, Liangyu; Li, Tianyu; Mo, Zengnan] Guangxi Med Univ, Affiliated Hosp 1, Inst Urol & Nephrol, Na...
推荐引用方式 GB/T 7714	Feng, Chao,Wang, Xi,Tao, Yuting,et al. Single-Cell Proteomic Analysis Dissects the Complexity of Tumor Microenvironment in Muscle Invasive Bladder Cancer[J],2021,13(21).
APA	Feng, Chao.,Wang, Xi.,Tao, Yuting.,Xie, Yuanliang.,Lai, Zhiyong.,...&Mo, Zengnan.(2021).Single-Cell Proteomic Analysis Dissects the Complexity of Tumor Microenvironment in Muscle Invasive Bladder Cancer.CANCERS,13(21).
MLA	Feng, Chao,et al."Single-Cell Proteomic Analysis Dissects the Complexity of Tumor Microenvironment in Muscle Invasive Bladder Cancer".CANCERS 13.21(2021).