干旱区生态环境知识资源中心(Arid): Genome-Wide Comprehensive Analysis of the GASA Gene Family in Populus

Arid

DOI	10.3390/ijms222212336
	Genome-Wide Comprehensive Analysis of the GASA Gene Family in Populus
	Han, Shuo; Jiao, Zhiyin; Niu, Meng-Xue; Yu, Xiao; Huang, Mengbo; Liu, Chao; Wang, Hou-Ling; Zhou, Yangyan; Mao, Wei; Wang, Xiaofei; Yin, Weilun; Xia, Xinli
通讯作者	Yin, WL ; Xia, XL (corresponding author), Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Lab Tree Breeding, Beijing 100083, Peoples R China.
来源期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
EISSN	1422-0067
出版年	2021
卷号	22 期号:22
英文摘要	Gibberellic acid-stimulated Arabidopsis (GASA) proteins, as cysteine-rich peptides (CRPs), play roles in development and reproduction and biotic and abiotic stresses. Although the GASA gene family has been identified in plants, the knowledge about GASAs in Populus euphratica, the woody model plant for studying abiotic stress, remains limited. Here, we referenced the well-sequenced Populus trichocarpa genome, and identified the GASAs in the whole genome of P. euphratica and P. trichocarpa. 21 candidate genes in P. trichocarpa and 19 candidate genes in P. euphratica were identified and categorized into three subfamilies by phylogenetic analysis. Most GASAs with signal peptides were located extracellularly. The GASA genes in Populus have experienced multiple gene duplication events, especially in the subfamily A. The evolution of the subfamily A, with the largest number of members, can be attributed to whole-genome duplication (WGD) and tandem duplication (TD). Collinearity analysis showed that WGD genes played a leading role in the evolution of GASA genes subfamily B. The expression patterns of P. trichocarpa and P. euphratica were investigated using the PlantGenIE database and the real-time quantitative PCR (qRT-PCR), respectively. GASA genes in P. trichocarpa and P. euphratica were mainly expressed in young tissues and organs, and almost rarely expressed in mature leaves. GASA genes in P. euphratica leaves were also widely involved in hormone responses and drought stress responses. GUS activity assay showed that PeuGASA15 was widely present in various organs of the plant, especially in vascular bundles, and was induced by auxin and inhibited by mannitol dramatically. In summary, this present study provides a theoretical foundation for further research on the function of GASA genes in P. euphratica.
英文关键词	GASA genes bioinformatics Populus growth and development phytohormone
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000724614300001
WOS关键词	ABSCISIC-ACID ; RHIZOCTONIA-SOLANI ; ENHANCES TOLERANCE ; SEED-GERMINATION ; QUANTITATIVE PCR ; DESERT POPLAR ; RICH PROTEIN ; EXPRESSION ; STRESS ; IDENTIFICATION
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/373759
作者单位	[Han, Shuo; Jiao, Zhiyin; Niu, Meng-Xue; Yu, Xiao; Huang, Mengbo; Liu, Chao; Wang, Hou-Ling; Yin, Weilun; Xia, Xinli] Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Lab Tree Breeding, Beijing 100083, Peoples R China; [Zhou, Yangyan; Mao, Wei; Wang, Xiaofei] Salver Acad Bot, Rizhao 276800, Peoples R China
推荐引用方式 GB/T 7714	Han, Shuo,Jiao, Zhiyin,Niu, Meng-Xue,et al. Genome-Wide Comprehensive Analysis of the GASA Gene Family in Populus[J],2021,22(22).
APA	Han, Shuo.,Jiao, Zhiyin.,Niu, Meng-Xue.,Yu, Xiao.,Huang, Mengbo.,...&Xia, Xinli.(2021).Genome-Wide Comprehensive Analysis of the GASA Gene Family in Populus.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(22).
MLA	Han, Shuo,et al."Genome-Wide Comprehensive Analysis of the GASA Gene Family in Populus".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.22(2021).