干旱区生态环境知识资源中心(Arid): Determination of Levamisole and Mebendazole and Its Two Metabolite Residues in Three Poultry Species by HPLC-MS/MS

Arid

DOI	10.3390/foods10112841
	Determination of Levamisole and Mebendazole and Its Two Metabolite Residues in Three Poultry Species by HPLC-MS/MS
	Gao, Pengfei; Zhang, Peiyang; Guo, Yawen; He, Zhaoyuan; Dong, Yuhao; Tang, Yayun; Guan, Fanxun; Zhang, Tao; Xie, Kaizhou
通讯作者	Xie, KZ (corresponding author), Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Jiangsu, Peoples R China. ; Xie, KZ (corresponding author), Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Yangzhou 225009, Jiangsu, Peoples R China.
来源期刊	FOODS
EISSN	2304-8158
出版年	2021
卷号	10 期号:11
英文摘要	A high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed to simultaneously analyze levamisole (LMS) and mebendazole (MBZ) and its two metabolites, 5-hydroxymebendazole (HMBZ) and 2-amino-5-benzoylbenzimidazole (AMBZ), in poultry muscle (chicken, duck and goose). In the sample preparation process, basic ethyl acetate was used as the extraction agent, and the extracted samples were back-extracted with hydrochloric acid, purified by Oasis MCX solid-phase extraction (SPE) cartridges, and reconstituted in the initial mobile phase after being blown dry with nitrogen. Chromatographic separation was performed on an Xbridge C-18 column (4.6 mm x 150 mm, 5 mu m) with 0.1% formic acid in water and acetonitrile as the mobile phases, and gradient elution was performed at a flow rate of 0.6 mL/min and a column temperature of 35 & DEG;C. In blank poultry muscle samples, the spiked concentrations of LMS, MBZ, HMBZ, and AMBZ were within the range of the limit of quantitation (LOQ) to 25 mu g/kg. The peak areas of the four target drugs had a good linear relationship with the concentration, and the determination coefficient (R-2) values were higher than 0.9990. The average recoveries of LMS, MBZ, HMBZ, and AMBZ were 86.77-96.94%; the intraday relative standard deviations (RSDs) were 1.75-4.99% at LOQ, 0.5 maximum residue limit (MRL), 1.0 MRL, and 2.0 MRL; the interday RSDs were 2.54-5.52%; and the LODs and LOQs were 0.04-0.30 mu g/kg and 0.12-0.80 mu g/kg, respectively.
英文关键词	chicken meat goose meat duck meat veterinary drugs validation method
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000725169200001
WOS关键词	TANDEM MASS-SPECTROMETRY ; VETERINARY DRUGS ; LIQUID-EXTRACTION ; BENZIMIDAZOLE ANTHELMINTICS ; CHROMATOGRAPHY ; MILK ; QUANTIFICATION ; TETRATHYRIDIA ; VALIDATION ; MULTICLASS
WOS类目	Food Science & Technology
WOS研究方向	Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/373749
作者单位	[Gao, Pengfei; Zhang, Peiyang; Guo, Yawen; He, Zhaoyuan; Tang, Yayun; Guan, Fanxun; Zhang, Tao; Xie, Kaizhou] Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Jiangsu, Peoples R China; [Gao, Pengfei; Zhang, Peiyang; Guo, Yawen; He, Zhaoyuan; Tang, Yayun; Guan, Fanxun; Zhang, Tao; Xie, Kaizhou] Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Yangzhou 225009, Jiangsu, Peoples R China; [Dong, Yuhao] Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China
推荐引用方式 GB/T 7714	Gao, Pengfei,Zhang, Peiyang,Guo, Yawen,et al. Determination of Levamisole and Mebendazole and Its Two Metabolite Residues in Three Poultry Species by HPLC-MS/MS[J],2021,10(11).
APA	Gao, Pengfei.,Zhang, Peiyang.,Guo, Yawen.,He, Zhaoyuan.,Dong, Yuhao.,...&Xie, Kaizhou.(2021).Determination of Levamisole and Mebendazole and Its Two Metabolite Residues in Three Poultry Species by HPLC-MS/MS.FOODS,10(11).
MLA	Gao, Pengfei,et al."Determination of Levamisole and Mebendazole and Its Two Metabolite Residues in Three Poultry Species by HPLC-MS/MS".FOODS 10.11(2021).