干旱区生态环境知识资源中心(Arid): Functional and Taxonomic Effects of Organic Amendments on the Restoration of Semiarid Quarry Soils

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	Functional and Taxonomic Effects of Organic Amendments on the Restoration of Semiarid Quarry Soils
	Miralles, Isabel; Ortega, Raul; Comeau, Andre M.
通讯作者	Miralles, I (corresponding author), Univ Almeria, Dept Agron, Almeria, Spain. ; Miralles, I (corresponding author), Univ Almeria, Ctr Intens Mediterranean Agrosyst & Agrifood Biot, Almeria, Spain.
来源期刊	MSYSTEMS
ISSN	2379-5077
出版年	2021
卷号	6 期号:6
英文摘要	The application of organic amendments to mining soils has been shown to be a successful method of restoration, improving key physicochemical soil properties. However, there is a lack of a clear understanding of the soil bacterial community taxonomic and functional changes that are brought about by these treatments. We present further metagenomic sequencing (MGS) profiling of the effects of different restoration treatments applied to degraded, arid quarry soils in southern Spain which had previously been profiled only with 16S rRNA gene (16S) and physicochemical analyses. Both taxonomic and functional MGS profiles showed clear separation of organic treatment amendments from control samples, and although taxonomic differences were quite clear, functional redundancy was higher than expected and the majority of the latter signal came from the aggregation of minor (<0.1%) community differences. Significant taxonomic differences were seen with the presumably less-biased MGS-for example, the phylum Actinobacteria and the two genera Chloracidobacterium (Acidobacteria) and Paenibacillus (Firmicutes) were determined to be major players by the MGS and this was consistent with their potential functional roles. The former phylum was much less present, and the latter two genera were either minor components or not detected in the 16S data. Mapping of reads to MetaCyc/BioCyc categories showed overall slightly higher biosynthesis and degradation capabilities in all treatments versus control soils, with sewage amendments showing highest values and vegetable-based amendments being at intermediate levels, matching higher nutrient levels, respiration rates, enzyme activities, and bacterial biomass previously observed in the treated soils. IMPORTANCE The restoration of soils impacted by human activities poses specific challenges regarding the reestablishment of functional microbial communities which will further support the reintroduction of plant species. Organic fertilizers, originating from either treated sewage or vegetable wastes, have shown promise in restoration experiments; however, we still do not have a clear understanding of the functional and taxonomic changes that occur during these treatments. We used metagenomics to profile restoration treatments applied to degraded, arid quarry soils in southern Spain. We found that the assortments of individual functions and taxa within each soil could clearly identify treatments, while at the same time they demonstrated high functional redundancy. Functions grouped into higher pathways tended to match physicochemical measurements made on the same soils. In contrast, significant taxonomic differences were seen when the treatments were previously studied with a single marker gene, highlighting the advantage of metagenomic analysis for complex soil communities.
英文关键词	shotgun metagenomics functional profiles bacterial taxonomy soil restoration sewage sludge compost semiarid mining soils
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000734744600004
WOS关键词	LAND-USE ; SP NOV. ; MICROBIAL COMMUNITIES ; BACTERIAL DIVERSITY ; METAGENOMIC ANALYSIS ; HOT-SPRINGS ; REDUNDANCY ; BIODIVERSITY ; BIOGEOGRAPHY ; DYNAMICS
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/373682
作者单位	[Miralles, Isabel; Ortega, Raul] Univ Almeria, Dept Agron, Almeria, Spain; [Miralles, Isabel; Ortega, Raul] Univ Almeria, Ctr Intens Mediterranean Agrosyst & Agrifood Biot, Almeria, Spain; [Comeau, Andre M.] Dalhousie Univ, Integrated Microbiome Resource IMR, Halifax, NS, Canada
推荐引用方式 GB/T 7714	Miralles, Isabel,Ortega, Raul,Comeau, Andre M.. Functional and Taxonomic Effects of Organic Amendments on the Restoration of Semiarid Quarry Soils[J],2021,6(6).
APA	Miralles, Isabel,Ortega, Raul,&Comeau, Andre M..(2021).Functional and Taxonomic Effects of Organic Amendments on the Restoration of Semiarid Quarry Soils.MSYSTEMS,6(6).
MLA	Miralles, Isabel,et al."Functional and Taxonomic Effects of Organic Amendments on the Restoration of Semiarid Quarry Soils".MSYSTEMS 6.6(2021).