干旱区生态环境知识资源中心(Arid): Immunomodulatory and Cytotoxic Properties of Natural Triterpenoids Isolated from Grewia flavescens Juss

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DOI	10.4103/pm.pm_235_20
	Immunomodulatory and Cytotoxic Properties of Natural Triterpenoids Isolated from Grewia flavescens Juss
	ELhassan, Gihan O. M.; Yagi, Sakina; Mesaik, M. Ahmed; Mohan, Syam; Alhazmi, Hassan A.; Al-Bratty, Mohammed; Al-Amri, Marai M.; Khalid, Asaad
通讯作者	Khalid, A (corresponding author), Jazan Univ, Subst Abuse & Toxicol Res Ctr, POB 114, Jazan 45142, Saudi Arabia.
来源期刊	PHARMACOGNOSY MAGAZINE
ISSN	0973-1296
EISSN	0976-4062
出版年	2021
卷号	17 期号:5 页码:S9-S14
英文摘要	Background: Grewia flavescens Juss (Malvaceae) is a species that is distributed throughout semi-arid and sub-humid tropical areas of Africa, Saudi Arabia, Yemen, and India. It is used in various traditional herbal practices for the treatment of various disease conditions. Objectives: The present investigation was undertaken to examine the immunomodulatory and cytotoxic properties of natural triterpenoids isolated from the stem bark of G. flavescens. Materials and Methods: The immunomodulatory activity was measured using oxidative burst chemiluminescence and phytohemagglutinin stimulated T-cell proliferation assay. The cytotoxicity was measured using MTT assay. One new triterpenoid (29 [30]-lupene-3, 20-diol; 3 beta-form, 29-Aldehyde [4]) in addition to four known ones; lupeol (1), beta-sitosterol (2), betulin (3), and beta-sitosterol glucopyranoside (5), have been isolated from the stem bark of G. flavescens and identified using Fourier-transform infrared spectroscopy, ultraviolet, and NMR techniques. Results: Chemiluminescence experiments showed that no compound exerted an inhibitory effect on reactive oxygen species production. However, 29 (30)-lupene-3, 20-diol; 3 beta-form, 29-Aldehyde (4) significantly repressed the cell proliferation activity with an IC50 of 8.7 mu g/mL, whereas, beta-sitosterol glucopyranoside (5) and beta-sitosterol (2) showed moderate inhibitory activities (IC50 of 16.7 and 23.6 mu g/mL, respectively) compared to the standard drug prednisolone (0.2 mu g/mL). Betulin (3) revealed the highest cytotoxicity activity toward Hela cancer cells, followed by 29 (30)-lupene-3, 20-diol; 3 beta-form, 29-Aldehyde (4), and beta-sitosterol (2) with IC50 11.10, 16.5 and 21.51 mu g/ml respectively. Conclusion: The present investigation supports the traditional use of this plant in treating various diseases and it has a good potential for future studies based on animal model based, which could bring more mechanistic facts about this plant's activity.
英文关键词	Cytotoxicity Grewia flavescens Juss immunomodulation oxidative burst T-cell proliferation triterpenoids
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000725298400002
WOS关键词	LUPANE-TYPE TRITERPENES ; CONSTITUENTS ; AGENTS ; CHEMILUMINESCENCE ; PROLIFERATION ; SITOSTEROL ; BETULIN ; BLOOD ; CELLS
WOS类目	Chemistry, Medicinal
WOS研究方向	Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/373577
作者单位	[ELhassan, Gihan O. M.; Yagi, Sakina] Univ Khartoum, Fac Sci, Dept Bot, Khartoum, Sudan; [Mesaik, M. Ahmed; Al-Amri, Marai M.] Univ Tabuk, Fac Med, Tabuk, Saudi Arabia; [Mesaik, M. Ahmed] Univ Karachi, Int Ctr Chem & Biol Sci, Dr Panjwani Ctr Mol Med & Drug Res, Karachi, Pakistan; [Mohan, Syam; Alhazmi, Hassan A.; Khalid, Asaad] Jazan Univ, Subst Abuse & Toxicol Res Ctr, POB 114, Jazan 45142, Saudi Arabia; [Al-Bratty, Mohammed] Jazan Univ, Coll Pharm, Dept Pharmaceut Chem, Jazan, Saudi Arabia; [Khalid, Asaad] Med & Aromat Plants Res Inst, Natl Ctr Res, Khartoum, Sudan
推荐引用方式 GB/T 7714	ELhassan, Gihan O. M.,Yagi, Sakina,Mesaik, M. Ahmed,et al. Immunomodulatory and Cytotoxic Properties of Natural Triterpenoids Isolated from Grewia flavescens Juss[J],2021,17(5):S9-S14.
APA	ELhassan, Gihan O. M..,Yagi, Sakina.,Mesaik, M. Ahmed.,Mohan, Syam.,Alhazmi, Hassan A..,...&Khalid, Asaad.(2021).Immunomodulatory and Cytotoxic Properties of Natural Triterpenoids Isolated from Grewia flavescens Juss.PHARMACOGNOSY MAGAZINE,17(5),S9-S14.
MLA	ELhassan, Gihan O. M.,et al."Immunomodulatory and Cytotoxic Properties of Natural Triterpenoids Isolated from Grewia flavescens Juss".PHARMACOGNOSY MAGAZINE 17.5(2021):S9-S14.