干旱区生态环境知识资源中心(Arid): Micromonospora tarapacensis sp. nov., a bacterium isolated from a hypersaline lake

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DOI	10.1099/ijsem.0.005109
	Micromonospora tarapacensis sp. nov., a bacterium isolated from a hypersaline lake
	Villalobos, Alvaro S.; Wiese, Jutta; Borchert, Erik; Rahn, Tanja; Slaby, Beate M.; Steiner, Leon X.; Kunzel, Sven; Dorador, Cristina; Imhoff, Johannes F.
通讯作者	Wiese, J (corresponding author), GEOMAR Helmholtz Ctr Ocean Res Kiel, RD3 Res Unit Marine Symbioses, Kiel, Germany.
来源期刊	INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
ISSN	1466-5026
EISSN	1466-5034
出版年	2021
卷号	71 期号:11
英文摘要	Strain Llam7(T) was isolated from microbial mat samples from the hypersaline lake Salar de Llamara, located in Taracapa region in the hyper-arid core of the Atacama Desert (Chile). Phenotypic, chemotaxonomic and genomic traits were studied. Phylogenetic analyses based on 165 rRNA gene sequences assigned the strain to the family Micromonosporaceae with affiliation to the genera Micromonospora and Salinispora. Major fatty acids were C-17:1 omega 8c, iso-C-15:0, iso-C-16:0 and anteiso-C-17:0. The cell walls contained meso-diaminopimelic acid and LL-2,6 diaminopimelic acid (LL-DAP). while major whole-cell sugars were glucose, mannose, xylose and ribose. The major menaquinones were MK-9(H-4) and MK-9(H-6). As polar lipids phosphatidylglycerol, phos-phatidylethanolamine, diphosphatidylglycerol and several unidentified lipids, i.e. two glycolipids, one aminolipid. three phospho-lipids, one aminoglycolipid and one phosphoglycolipid, were detected. Genome sequencing revealed a genome size of 6.894 Mb and a DNA G+C content of 71.4 mol%. Phylogenetic analyses with complete genome sequences positioned strain Llam7(T) within the family Micromonosporaceae forming a distinct cluster with Micromonospora (former Xiangella) phaseoli DSM 45730(T). This cluster is related to Micromonospora pelagivivens KJ-029(T), Micromonospora craterilacus NA12(T), and Micromonospora craniellae LHW63014(T) as well as to all members of the former genera Verrucosispora and Jishengella, which were re-classified as members of the genus Micromonospora, forming a clade distinct from the genus Salinispora. Pairwise whole genome average nucleotide identity (ANI) values, digital DNA-DNA hybridization (dDDH) values, the presence of the diamino acid LL-DAP, and the composition of whole sugars and polar lipids indicate that Llam7(T) represents a novel species, for which the name Micromono-spora tarapacensis sp. nov. is proposed, with Llam7(T) (=DSM 109510(T),=LMG 31023(T)) as the type strain.
英文关键词	Actinobacteria Atacama hypersaline Lake Micromonospora tarapacensis polyphasic taxonomy
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000729309700010
WOS关键词	INTRAGENERIC RELATIONSHIPS ; LIPID-COMPOSITION ; SEQUENCE ; ACTINOMYCETE ; CLUSTER ; VERSION
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/373563
作者单位	[Villalobos, Alvaro S.; Wiese, Jutta; Borchert, Erik; Rahn, Tanja; Slaby, Beate M.; Steiner, Leon X.; Imhoff, Johannes F.] GEOMAR Helmholtz Ctr Ocean Res Kiel, RD3 Res Unit Marine Symbioses, Kiel, Germany; [Villalobos, Alvaro S.] Pontificia Univ Catolica Valparaiso, Escuela Ciencias Mar, Valparaiso, Chile; [Kunzel, Sven] Max Planck Inst Evolutionary Biol, Plon, Germany; [Dorador, Cristina] Univ Antofagasta, Lab Complejidad Microbiana & Ecol Func, Fac Ciencias Mar & Recursos Biol, Antofagasta, Chile; [Dorador, Cristina] Univ Antofagasta, Dept Biotecnol, Fac Ciencias Mar & Recursos Biol, Antofagasta, Chile
推荐引用方式 GB/T 7714	Villalobos, Alvaro S.,Wiese, Jutta,Borchert, Erik,et al. Micromonospora tarapacensis sp. nov., a bacterium isolated from a hypersaline lake[J],2021,71(11).
APA	Villalobos, Alvaro S..,Wiese, Jutta.,Borchert, Erik.,Rahn, Tanja.,Slaby, Beate M..,...&Imhoff, Johannes F..(2021).Micromonospora tarapacensis sp. nov., a bacterium isolated from a hypersaline lake.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,71(11).
MLA	Villalobos, Alvaro S.,et al."Micromonospora tarapacensis sp. nov., a bacterium isolated from a hypersaline lake".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 71.11(2021).