干旱区生态环境知识资源中心(Arid): Micropropagation of the small sacasil organ cactus (Echinocereus poselgeri Lem.) (Fam.: Cactaceae)

Arid

DOI	10.17660/ActaHortic.2020.1288.19
	Micropropagation of the small sacasil organ cactus (Echinocereus poselgeri Lem.) (Fam.: Cactaceae)
	Villavicencio Gutierrez, E. E.; Arellano-Ostoa, G.; Belmontes Diaz, V. A.
通讯作者	Gutierrez, EEV (corresponding author), INIFAP, Campo Expt Saltillo CIRNE, Mexico City, DF, Mexico.
会议名称	9th International Symposium on New Ornamental Crops
会议日期	SEP 30-OCT 03, 2019
会议地点	Guadalajara, MEXICO
英文摘要	The small sacasil cactus (Echinocereus poselgeri Lem.; Cactaceae), is native to northern Mexico. Grows in the Chihuahuan Desert in limestone soils, in extremely dry conditions. This species has been little studied and due to its morphological characteristics (thin cylindrical stems, dark bluish blue color, 30 to 60 cm high, with a diameter of 2 cm and white radial spines on its ribs with a very bright magenta pink bloom), represent a new interesting ornamental option. The objective of this work was to generate an efficient regeneration protocol for the massive production of the species. During the fall of 2017 seeds were collected from the eastern region of the municipality of Guadalcazar in San Luis Potosi. An aseptic culture was established in the Laboratorio de Cultivos de Tejidos Vegetales (LCTV) of the Campo Experimental Saltillo CIRNE-INIFAP, the multiplication and rooting stages were established in 2018-2019 in MS medium with 50% macronutrients. Multiplication stage: four concentrations (2.5, 5.0, 7.5 and 10 mg L-1) of the cytokinins 6-benzylaminopurine (BAP) and kinetin 6-furfurylaminopurine (KIN) and the auxins naphthaleneacetic acid (NAA) and indolebutyric acid (IBA) keeping a relationship 10:1. The number of shoots (SN) and its size (SS) were measured. Rooting stage: two concentrations of sucrose (2 and 4%) and two auxins, indole acetic acid ( IAA) and indole butyric acid (IBA) (1 and 2 mg L-1) were tested. The number and length of roots shoot(-1) were measured. At each stage, five explants (nodal sections) were established per bottle with 8 replicates per treatment in a completely randomized experimental design with factorial arrangement. An analysis of variance (ANOVA) and a Tukey multiple range test (P=0.05) were performed using the Statistical Analysis System (SAS) 14.1 software. The best multiplication rate (3.73 shoots explant(-1)) and height (5.05 mm) were obtained with the treatment of 7.5 mg L-1 of KIN + 0.75 mg L-1 of IBA. The rooting of shoots was obtained when increasing the concentration of sucrose and IBA, generating root production by vitroplant of 80% with respect to the treatment without phytohormones.
来源出版物	IX INTERNATIONAL SYMPOSIUM ON NEW ORNAMENTAL CROPS
ISSN	0567-7572
EISSN	2406-6168
出版年	2020
卷号	1288
页码	123-130
ISBN	978-94-62612-86-0
出版者	INT SOC HORTICULTURAL SCIENCE
类型	Proceedings Paper
语种	英语
收录类别	CPCI-S
WOS记录号	WOS:000706487100019
WOS关键词	IN-VITRO PROPAGATION
WOS类目	Agronomy ; Horticulture
WOS研究方向	Agriculture
资源类型	会议论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/370309
作者单位	[Villavicencio Gutierrez, E. E.] INIFAP, Campo Expt Saltillo CIRNE, Mexico City, DF, Mexico; [Arellano-Ostoa, G.] Colegio Postgrad, Campus Montecillo, Mexico City, DF, Mexico; [Belmontes Diaz, V. A.] Univ Autonoma Agr Antonio Narro, Dept Forestal, Saltillo, Coahuila, Mexico
推荐引用方式 GB/T 7714	Villavicencio Gutierrez, E. E.,Arellano-Ostoa, G.,Belmontes Diaz, V. A.. Micropropagation of the small sacasil organ cactus (Echinocereus poselgeri Lem.) (Fam.: Cactaceae)[C]:INT SOC HORTICULTURAL SCIENCE,2020:123-130.