干旱区生态环境知识资源中心(Arid): Argylia radiata polyploidization, first approach for plant breeding

Arid

DOI	10.17660/ActaHortic.2020.1288.8
	Argylia radiata polyploidization, first approach for plant breeding
	Morales-Tapia, P.; Gambardella, M.; Barba-Gonzalez, R.
通讯作者	Morales-Tapia, P (corresponding author), Pontificia Univ Catolica Chile, Fac Agron & Ingn Forestal, Santiago, Chile.
会议名称	9th International Symposium on New Ornamental Crops
会议日期	SEP 30-OCT 03, 2019
会议地点	Guadalajara, MEXICO
英文摘要	Argylia radiata (L.) D. Don (Fam.: Bignoniaceae) is an herbaceous perennial plant, native from the North of Chile and part of Flowering Desert. This understudied species has potential as an ornamental crop because of its blue-green foliage and multiple floral stems with trumpet flowers. As first approach of the A. radiata breeding process, polyploidization trials were run. Two induction agents, oryzalin and nitrous oxide, were tested at in vitro level. Under sterile conditions, A. radiata microplants were put inside Eppendorf tubes with oryzalin solution. Two concentrations and two times of exposition were assayed: 0.01 mg L-1 during 24 h (T1); 0.01 mg L-1 during 48 h (T2); 0.05 mg L-1 during 24 h (T3); 0.05 mg L-1 during 48 h (T4). Regards to the experiments with nitrous oxide, microplants of A. radiata were put on sterile glass tubes and located inside a gas chamber. The nitrous oxide was injected at 5 atm during 24 h (T5) and 48 h (T6). For all treatments, five plants were used. After the induction essays, the plants were transferred to a free hormones medium during three weeks and the regenerated shoots were cut, leaving only one bulb explant(-1). This process was repeated twice. The new microplants, regenerated from those buds, were treated as independent lines (56 lines were obtained), and they were transferred to fresh medium every 12 weeks during 1 year. No plants from T3 and T4 were possible to regenerate. After that period, a small piece of tissue, from each line, was analyzed by flow cytometry, detecting 1 mixaploid (75% 2n and 25% 4n) from T2. This material could be used in the future to obtain a complete tetraploid plant by bud's isolation, to generate polyploid pollen or it can be used as part of a breeding program.
来源出版物	IX INTERNATIONAL SYMPOSIUM ON NEW ORNAMENTAL CROPS
ISSN	0567-7572
EISSN	2406-6168
出版年	2020
卷号	1288
页码	59-63
ISBN	978-94-62612-86-0
出版者	INT SOC HORTICULTURAL SCIENCE
类型	Proceedings Paper
语种	英语
收录类别	CPCI-S
WOS记录号	WOS:000706487100008
WOS类目	Agronomy ; Horticulture
WOS研究方向	Agriculture
资源类型	会议论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/370308
作者单位	[Morales-Tapia, P.; Gambardella, M.] Pontificia Univ Catolica Chile, Fac Agron & Ingn Forestal, Santiago, Chile; [Barba-Gonzalez, R.] Ctr Invest & Asistencia Tecnol & Diseno Estado Ja, Unidad Zapopan, Zapopan, Jalisco, Mexico
推荐引用方式 GB/T 7714	Morales-Tapia, P.,Gambardella, M.,Barba-Gonzalez, R.. Argylia radiata polyploidization, first approach for plant breeding[C]:INT SOC HORTICULTURAL SCIENCE,2020:59-63.