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	Bioprospecting of actinobacteria from the Andaman marine ecosystem: Isolation, antagonistic potential, and taxonomy of potential strain
	Manisha, M.; Mithali, J.; Vijayalakshmi, G.; Gopikrishnan, V.; Masilamaniselvam, M.; Radhakrishnan, M.
通讯作者	Radhakrishnan, M (corresponding author), Sathyabama Inst Sci & Technol, Col Dr Jeppiaar Res Pk, Ctr Drug Discovery & Dev, Chennai, Tamil Nadu, India.
会议名称	National Symposium on Recent Advances in Marine Sciences
会议日期	SEP 13-14, 2017
会议地点	Chennai, INDIA
英文摘要	The present study reports on the bioprospecting of actinobacteria from the Andaman marine ecosystem with special reference to antimicrobial compounds. Twenty-eight morphologically different actinobacterial cultures were isolated from rhizosphere sediments collected from five mangrove plants in Dhaninallah, Middle Andaman. Twenty out of 28 actinobacterial strains showed antagonistic activity in which 13 strains showed broad-spectrum activity against Gram positive and Gram negative bacterial pathogens. Strain AMA37, which showed the maximum zone of inhibition, was selected as the potential strain for further studies. Bioactive metabolites were produced by solid-state fermentation and the methanol extracts of AMA 37 showed broad-spectrum activity against clinical, fish, poultry, and fungal pathogens. The MIC (minimal inhibitory concentration) of crude extract ranged between 62.5 and 250 mu g/ml. On the basis of phenotypic and molecular characteristics, the strain AMA37 was identified as a novel species of the genus Streptomyces as it showed 97% similarity with the closely related strain Streptomyces djakartensis. The 16s rRNA sequence was deposited in GenBank with accession number KU999997. The present study revealed that Streptomyces sp. AMA37 is a promising source for isolation of bioactive compounds and should pave the way for clinical, poultry, and aquaculture applications.
英文关键词	Streptomyces sp. Antimicrobial activity Mangrove sediments Andaman Islands MIC
来源出版物	INDIAN JOURNAL OF GEO-MARINE SCIENCES
ISSN	0379-5136
EISSN	0975-1033
出版年	2019
卷号	48
期号	8
页码	1312-1318
出版者	NATL INST SCIENCE COMMUNICATION-NISCAIR
类型	Article; Proceedings Paper
语种	英语
收录类别	CPCI-S
WOS记录号	WOS:000498626400023
WOS关键词	NATURAL-PRODUCTS ; DESERT SOIL ; PURIFICATION ; ANTIBIOTICS ; COMPOUND
WOS类目	Oceanography
WOS研究方向	Oceanography
资源类型	会议论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/369963
作者单位	[Manisha, M.; Mithali, J.; Masilamaniselvam, M.] Sathyabama Inst Sci & Technol, Dept Biotechnol, Chennai, Tamil Nadu, India; [Vijayalakshmi, G.; Gopikrishnan, V.; Radhakrishnan, M.] Sathyabama Inst Sci & Technol, Col Dr Jeppiaar Res Pk, Ctr Drug Discovery & Dev, Chennai, Tamil Nadu, India
推荐引用方式 GB/T 7714	Manisha, M.,Mithali, J.,Vijayalakshmi, G.,et al. Bioprospecting of actinobacteria from the Andaman marine ecosystem: Isolation, antagonistic potential, and taxonomy of potential strain[C]:NATL INST SCIENCE COMMUNICATION-NISCAIR,2019:1312-1318.