干旱区生态环境知识资源中心(Arid): De Novo Transcriptome Assembly of Agave H11648 by Illumina Sequencing and Identification of Cellulose Synthase Genes in Agave Species

Arid

DOI	10.3390/genes10020103
	De Novo Transcriptome Assembly of Agave H11648 by Illumina Sequencing and Identification of Cellulose Synthase Genes in Agave Species
	Huang, Xing; Xiao, Mei; Xi, Jingen; He, Chunping; Zheng, Jinlong; Chen, Helong; Gao, Jianming; Zhang, Shiqing; Wu, Weihuai; Liang, Yanqiong; Xie, Li; Yi, Kexian
通讯作者	Yi, KX (corresponding author), Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou 571101, Hainan, Peoples R China.
来源期刊	GENES
EISSN	2073-4425
出版年	2019
卷号	10 期号:2
英文摘要	Agave plants are important crassulacean acid metabolism (CAM) plants with multiple agricultural uses, such as being used in tequila and fiber production. Agave hybrid H11648 ((A. amaniensis Trel. and Nowell x A. angustifolia Haw.) x A. amaniensis) is the main cultivated Agave species for fiber production in large tropical areas around the world. In this study, we conducted a transcriptome analysis of A. H11648. About 49.25 million clean reads were obtained by Illumina paired-end sequencing. De novo assembly produced 148,046 unigenes with more than 40% annotated in public databases, or matched homologs in model plants. More homologous gene pairs were found in Asparagus genome than in Arabidopsis or rice, which indicated a close evolutionary relationship between Asparagus and A. H11648. CAM-related gene families were also characterized as previously reported in A. americana. We further identified 12 cellulose synthase genes (CesA) in Asparagus genome and 38 CesA sequences from A. H11648, A. americana, A. deserti and A. tequilana. The full-length CesA genes were used as references for the cloning and assembly of their homologs in other Agave species. As a result, we obtained CesA1/3/4/5/7 genes with full-length coding region in the four Agave species. Phylogenetic and expression analysis revealed a conserved evolutionary pattern, which could not explain the distinct fiber traits in different Agave species. We inferred that transcriptional regulation might be responsible for Agave fiber development. This study represents the transcriptome of A. H11648, which would expand the number of Agave genes and benefit relevant studies of Agave fiber development.
英文关键词	RNA-seq crassulacean acid metabolism Agave cellulose synthase Asparagus gene clone phylogeny gene expression
类型	Article
语种	英语
开放获取类型	gold, Green Published, Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000460742800032
WOS关键词	CRASSULACEAN ACID METABOLISM ; BIOENERGY PRODUCTION ; EXPRESSION ; ASPARAGACEAE ; PLANTS ; CAM
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/369891
作者单位	[Huang, Xing; Xi, Jingen; He, Chunping; Zheng, Jinlong; Wu, Weihuai; Liang, Yanqiong; Yi, Kexian] Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou 571101, Hainan, Peoples R China; [Xiao, Mei] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Hubei, Peoples R China; [Chen, Helong; Gao, Jianming; Zhang, Shiqing] Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China; [Xie, Li] Hainan Univ, Inst Trop Agr & Forestry, Haikou 570228, Hainan, Peoples R China
推荐引用方式 GB/T 7714	Huang, Xing,Xiao, Mei,Xi, Jingen,et al. De Novo Transcriptome Assembly of Agave H11648 by Illumina Sequencing and Identification of Cellulose Synthase Genes in Agave Species[J],2019,10(2).
APA	Huang, Xing.,Xiao, Mei.,Xi, Jingen.,He, Chunping.,Zheng, Jinlong.,...&Yi, Kexian.(2019).De Novo Transcriptome Assembly of Agave H11648 by Illumina Sequencing and Identification of Cellulose Synthase Genes in Agave Species.GENES,10(2).
MLA	Huang, Xing,et al."De Novo Transcriptome Assembly of Agave H11648 by Illumina Sequencing and Identification of Cellulose Synthase Genes in Agave Species".GENES 10.2(2019).