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DOI	10.1093/femsle/fnz177
	Validating biochemical features at the genome level in the Antarctic bacterium Hymenobacter sp. strain UV11
	Marizcurrena, Juan Jose; Herrera, Lorena M.; Costabile, Alicia; Morales, Danilo; Villadoniga, Carolina; Eizmendi, Agustina; Davyt, Danilo; Castro-Sowinski, Susana
通讯作者	Castro-Sowinski, S (corresponding author), Univ Republica, Fac Sci, Biochem & Mol Biol, Igua 4225, Montevideo 11400, Uruguay.
来源期刊	FEMS MICROBIOLOGY LETTERS
ISSN	0378-1097
EISSN	1574-6968
出版年	2019
卷号	366 期号:14
英文摘要	We present experimental data that complement and validate some biochemical features at the genome level in the UVC-resistant Antarctic bacterium Hymenobacter sp. UV11 strain. The genome was sequenced, assembled and annotated. It has 6 096 246 bp, a GC content of 60.6% and 5155 predicted genes. The secretome analysis, by combining in silico predictions with shotgun proteomics data, showed that UV11 strain produces extracellular proteases and carbohydrases with potential biotechnological uses. We observed the formation of outer membrane vesicles, mesosomes and carbon-storage compounds by using transmission electron microscopy. The in silico analysis of the genome revealed the presence of genes involved in the metabolism of glycogen-like molecules and starch. By HPLC-UV-Vis analysis and 1H-NMR spectra, we verified that strain UV11 produces xanthophyll-like carotenoids such as 2'-hydroxyflexixanthin, and the in silico analysis showed that this bacterium has genes involved in the biosynthesis of cathaxanthin, zeaxanthin and astaxanthin. We also found genes involved in the repair of UV-damaged DNA such as a photolyase, the nucleotide excision repair system and the production of ATP-dependent proteases that are important cellular components involved in the endurance to physiological stresses. This information will help us to better understand the ecological role played by Hymenobacter strains in the extreme Antarctic environment.
英文关键词	Hymenobacter UV irradiation hydrolytic enzymes Antarctica pigments
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000493066300011
WOS关键词	RADIATION-RESISTANT BACTERIUM ; SP NOV. ; SEQUENCE ; PROTEASE ; DAMAGE ; FLAVOBACTERIUM ; XINJIANG ; DESERT ; MODEL ; KEGG
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/369859
作者单位	[Marizcurrena, Juan Jose; Herrera, Lorena M.; Costabile, Alicia; Morales, Danilo; Castro-Sowinski, Susana] Univ Republica, Fac Sci, Biochem & Mol Biol, Igua 4225, Montevideo 11400, Uruguay; [Villadoniga, Carolina; Castro-Sowinski, Susana] Univ Republica, Fac Sci, Hydrolyt Enzymes Lab, Igua 4225, Montevideo 11400, Uruguay; [Eizmendi, Agustina; Davyt, Danilo] Univ Republica, Fac Chem, Organ Chem Dept, Gen Flores 2124, Montevideo 11800, Uruguay
推荐引用方式 GB/T 7714	Marizcurrena, Juan Jose,Herrera, Lorena M.,Costabile, Alicia,et al. Validating biochemical features at the genome level in the Antarctic bacterium Hymenobacter sp. strain UV11[J],2019,366(14).
APA	Marizcurrena, Juan Jose.,Herrera, Lorena M..,Costabile, Alicia.,Morales, Danilo.,Villadoniga, Carolina.,...&Castro-Sowinski, Susana.(2019).Validating biochemical features at the genome level in the Antarctic bacterium Hymenobacter sp. strain UV11.FEMS MICROBIOLOGY LETTERS,366(14).
MLA	Marizcurrena, Juan Jose,et al."Validating biochemical features at the genome level in the Antarctic bacterium Hymenobacter sp. strain UV11".FEMS MICROBIOLOGY LETTERS 366.14(2019).