干旱区生态环境知识资源中心(Arid): Transepithelial transport of P-glycoprotein substrate by the Malpighian tubules of the desert locust

Arid

DOI	10.1371/journal.pone.0223569
	Transepithelial transport of P-glycoprotein substrate by the Malpighian tubules of the desert locust
	Rossi, Marta; De Battisti, Davide; Niven, Jeremy Edward
通讯作者	Rossi, M ; Niven, JE (corresponding author), Univ Sussex, Sch Life Sci, Brighton, E Sussex, England. ; Niven, JE (corresponding author), Univ Sussex, Ctr Computat Neurosci & Robot, Brighton, E Sussex, England.
来源期刊	PLOS ONE
ISSN	1932-6203
出版年	2019
卷号	14 期号:10
英文摘要	Extrusion of xenobiotics is essential for allowing animals to remove toxic substances present in their diet or generated as a biproduct of their metabolism. By transporting a wide range of potentially noxious substrates, active transporters of the ABC transporter family play an important role in xenobiotic extrusion. One such class of transporters are the multidrug resistance P-glycoprotein transporters. Here, we investigated P-glycoprotein transport in the Malpighian tubules of the desert locust (Schistocerca gregaria), a species whose diet includes plants that contain toxic secondary metabolites. To this end, we studied transporter physiology using a modified Ramsay assay in which ex vivo Malpighian tubules are incubated in different solutions containing the P-glycoprotein substrate dye rhodamine B in combination with different concentrations of the P-glycoprotein inhibitor verapamil. To determine the quantity of the P-glycoprotein substrate extruded we developed a simple and cheap method as an alternative to liquid chromatography-mass spectrometry, radiolabelled alkaloids or confocal microscopy. Our evidence shows that: (i) the Malpighian tubules contain a P-glycoprotein; (ii) tubule surface area is positively correlated with the tubule fluid secretion rate; and (iii) as the fluid secretion rate increases so too does the net extrusion of rhodamine B. We were able to quantify precisely the relationships between the fluid secretion, surface area, and net extrusion. We interpret these results in the context of the life history and foraging ecology of desert locusts. We argue that P-glycoproteins contribute to the removal of xenobiotic substances from the haemolymph, thereby enabling gregarious desert locusts to maintain toxicity through the ingestion of toxic plants without suffering the deleterious effects themselves.
类型	Article
语种	英语
开放获取类型	Green Published, Green Accepted, Green Submitted, gold
收录类别	SCI-E
WOS记录号	WOS:000532431700009
WOS关键词	BLOOD-BRAIN-BARRIER ; FLUID SECRETION ; SCHISTOCERCA-GREGARIA ; INSECTICIDE RESISTANCE ; MULTIDRUG-RESISTANCE ; DIURETIC HORMONE ; ACTIVE-TRANSPORT ; ORGANIC CATION ; AEDES-AEGYPTI ; IN-VITRO
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/369802
作者单位	[Rossi, Marta; Niven, Jeremy Edward] Univ Sussex, Sch Life Sci, Brighton, E Sussex, England; [De Battisti, Davide] Swansea Univ, Dept Biosci, Singleton Pk, Swansea, W Glam, Wales; [Niven, Jeremy Edward] Univ Sussex, Ctr Computat Neurosci & Robot, Brighton, E Sussex, England
推荐引用方式 GB/T 7714	Rossi, Marta,De Battisti, Davide,Niven, Jeremy Edward. Transepithelial transport of P-glycoprotein substrate by the Malpighian tubules of the desert locust[J],2019,14(10).
APA	Rossi, Marta,De Battisti, Davide,&Niven, Jeremy Edward.(2019).Transepithelial transport of P-glycoprotein substrate by the Malpighian tubules of the desert locust.PLOS ONE,14(10).
MLA	Rossi, Marta,et al."Transepithelial transport of P-glycoprotein substrate by the Malpighian tubules of the desert locust".PLOS ONE 14.10(2019).