干旱区生态环境知识资源中心(Arid): Constitutive expression of an A-5 subgroup member in the DREB transcription factor subfamily from Ammopiptanthus mongolicus enhanced abiotic stress tolerance and anthocyanin accumulation in transgenic Arabidopsis

Arid

DOI	10.1371/journal.pone.0224296
	Constitutive expression of an A-5 subgroup member in the DREB transcription factor subfamily from Ammopiptanthus mongolicus enhanced abiotic stress tolerance and anthocyanin accumulation in transgenic Arabidopsis
	Ren, Meiyan; Wang, Zhilin; Xue, Min; Wang, Xuefeng; Zhang, Feng; Zhang, Yu; Zhang, Wenjun; Wang, Maoyan
通讯作者	Wang, MY (corresponding author), Inner Mongolia Agr Univ, Coll Life Sci, Hohhot, Peoples R China.
来源期刊	PLOS ONE
ISSN	1932-6203
出版年	2019
卷号	14 期号:10
英文摘要	Dehydration-responsive element-binding (DREB) transcription factors (TFs) are key regulators of stress-inducible gene expression in plants. Anthocyanins, an important class of flavonoids, protect plants from reactive oxygen species produced under abiotic stresses. However, regulation of DREBs on anthocyanin accumulation is largely unknown. Here, an A-5 subgroup DREB gene (AmDREB3) isolated from Ammopiptanthus mongolicus, a desert broadleaf shrub with very high tolerance to harsh environments, was characterized in terms of both abiotic stress tolerance and anthocyanin accumulation. AmDREB3 does not contain the transcriptional repression motif EAR, and the protein was located in the nucleus and has transcriptional activation capacity. The transcription of AmDREB3 was differentially induced in the shoots and roots of A. mongolicus seedlings under drought, salt, heat, cold, ultraviolet B, and abscisic acid treatments. Moreover, the transcript levels in twigs, young leaves, and roots were higher than in other organs of A. mongolicus shrubs. Constitutively expressing AmDREB3 improved the tolerance of transgenic Arabidopsis to drought, high salinity and heat, likely by inducing the expression of certain stress-inducible genes. The transgenic Arabidopsis seedlings also exhibited an obvious purple coloration and significant increases in anthocyanin accumulation and/or oxidative stress tolerance under drought, salt, and heat stresses. These results suggest that the AmDREB3 TF may be an important positive regulator of both stress tolerance and anthocyanin accumulation.
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000532599700065
WOS关键词	SALT TOLERANCE ; COLD-HARDINESS ; EAR-MOTIF ; BINDING ; GENE ; DROUGHT ; BIOSYNTHESIS ; MECHANISM ; IMPACT ; PLANTS
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/369798
作者单位	[Ren, Meiyan; Wang, Zhilin; Xue, Min; Wang, Xuefeng; Zhang, Feng; Zhang, Yu; Zhang, Wenjun; Wang, Maoyan] Inner Mongolia Agr Univ, Coll Life Sci, Hohhot, Peoples R China
推荐引用方式 GB/T 7714	Ren, Meiyan,Wang, Zhilin,Xue, Min,et al. Constitutive expression of an A-5 subgroup member in the DREB transcription factor subfamily from Ammopiptanthus mongolicus enhanced abiotic stress tolerance and anthocyanin accumulation in transgenic Arabidopsis[J],2019,14(10).
APA	Ren, Meiyan.,Wang, Zhilin.,Xue, Min.,Wang, Xuefeng.,Zhang, Feng.,...&Wang, Maoyan.(2019).Constitutive expression of an A-5 subgroup member in the DREB transcription factor subfamily from Ammopiptanthus mongolicus enhanced abiotic stress tolerance and anthocyanin accumulation in transgenic Arabidopsis.PLOS ONE,14(10).
MLA	Ren, Meiyan,et al."Constitutive expression of an A-5 subgroup member in the DREB transcription factor subfamily from Ammopiptanthus mongolicus enhanced abiotic stress tolerance and anthocyanin accumulation in transgenic Arabidopsis".PLOS ONE 14.10(2019).