干旱区生态环境知识资源中心(Arid): Divulging diazotrophic bacterial community structure in Kuwait desert ecosystems and their N-2-fixation potential

Arid

DOI	10.1371/journal.pone.0220679
	Divulging diazotrophic bacterial community structure in Kuwait desert ecosystems and their N-2-fixation potential
	Suleiman, M. K.; Quoreshi, A. M.; Bhat, N. R.; Manuvel, A. J.; Sivadasan, M. T.
通讯作者	Quoreshi, AM (corresponding author), Kuwait Inst Sci Res, Environm & Life Sci Res Ctr, Desert Agr & Ecosyst Program, Safat, Kuwait.
来源期刊	PLOS ONE
ISSN	1932-6203
出版年	2019
卷号	14 期号:12
英文摘要	Kuwait is a semi-arid region with soils that are relatively nitrogen-poor. Thus, biological nitrogen fixation is an important natural process in which N-2-fixing bacteria (diazotrophs) convert atmospheric nitrogen into plant-usable forms such as ammonium and nitrate. Currently, there is limited information on free-living and root-associated nitrogen-fixing bacteria and their potential to fix nitrogen and aid natural plant communities in the Kuwait desert. In this study, free living N-2-fixing diazotrophs were enriched and isolated from the rhizosphere soil associated with three native keystone plant species; Rhanterium epapposum, Farsetia aegyptia, and Haloxylon salicornicum. Root-associated bacteria were isolated from the root nodules of Vachellia pachyceras. The result showed that the strains were clustered in five groups represented by class: gamma-proteobacteria, and alpha-proteobacteria; phyla: Actinobacteria being the most dominant, followed by phyla: Firmicutes, and class: beta-proteobacteria. This study initially identified 50 nitrogen-fixers by16S rRNA gene sequencing, of which 78% were confirmed to be nitrogen-fixers using the acetylene reduction assay. Among the nitrogen fixers identified, the genus Rhizobium was predominant in the rhizosphere soil of R. epapposum and H. salicornicum, whereas Pseudomonas was predominant in the rhizosphere soil of F. aegyptia, The species Agrobacterium tumefaciens was mainly found to be dominant among the root nodules of V. pachyceras and followed by Cellulomonas, Bacillus, and Pseudomonas genera as root-associated bacteria. The variety of diazotrophs revealed in this study, signifying the enormous importance of free-living and root-associated bacteria in extreme conditions and suggesting potential ecological importance of diazotrophs in arid ecosystem. To our knowledge, this study is the first to use culture-based isolation, molecular identification, and evaluation of N-2-fixing ability to detail diazotroph diversity in Kuwaiti desert soils.
类型	Article
语种	英语
开放获取类型	Green Published, Green Submitted, gold
收录类别	SCI-E
WOS记录号	WOS:000515084600003
WOS关键词	NITROGEN-FIXING MICROORGANISMS ; AGROBACTERIUM-TUMEFACIENS ; RHIZOBIUM-RADIOBACTER ; DIVERSITY ; RHIZOSPHERE ; FIXATION ; MICROBIOTA ; RHIZOGENES ; PATTERNS ; STRAINS
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/369734
作者单位	[Suleiman, M. K.; Quoreshi, A. M.; Bhat, N. R.; Manuvel, A. J.; Sivadasan, M. T.] Kuwait Inst Sci Res, Environm & Life Sci Res Ctr, Desert Agr & Ecosyst Program, Safat, Kuwait
推荐引用方式 GB/T 7714	Suleiman, M. K.,Quoreshi, A. M.,Bhat, N. R.,et al. Divulging diazotrophic bacterial community structure in Kuwait desert ecosystems and their N-2-fixation potential[J],2019,14(12).
APA	Suleiman, M. K.,Quoreshi, A. M.,Bhat, N. R.,Manuvel, A. J.,&Sivadasan, M. T..(2019).Divulging diazotrophic bacterial community structure in Kuwait desert ecosystems and their N-2-fixation potential.PLOS ONE,14(12).
MLA	Suleiman, M. K.,et al."Divulging diazotrophic bacterial community structure in Kuwait desert ecosystems and their N-2-fixation potential".PLOS ONE 14.12(2019).