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	Antifungal and anti-aflatoxin efficacy of biogenic silver nanoparticles produced by Aspergillus species: Molecular study
	Zaban, Mayasar Ibrahim; Azim, Nahla Shazli Abdel; Abd El-Aziz, Abeer Ramadan Mohamed
通讯作者	Abd El-Aziz, ARM (corresponding author), King Saud Univ, Bot & Microbiol Dept, Coll Sci, Riyadh, Saudi Arabia.
来源期刊	PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES
ISSN	1011-601X
出版年	2019
卷号	32 期号:5 页码:2509-2526
英文摘要	Date palm (Phoenix dactylifera L.) is considered the main crop in deserts and arid areas such as Saudi Arabia. Fifteen species belonging to 7 fungal genera were isolated from date palm rhizosphere soil at Riyadh, Saudi Arabia. Twenty-one isolates of Aspergillus spp. were used in producing silver nanoparticles (SNPs), five of A. flavus, nine of A. niger and seven of A. terreus. Synthesis of SNPs by these fungi is emerging as an important branch of nanotechnology due to it'secofriendly, safe and cost-effective nature. SNPs have been characterized by UV-Visible Spectrophotometer and Transmission Electron Microscope (TEM). In order to increase the yield of biosynthesized SNPs of desired shape and size, it is necessary to control the cultural and physical parameters during the synthesis. We reported the optimum synthesis of SNPs on a liquid medium at 1.5mM of silver nitrate, pH 9 and 26 degrees C after 96 hours. Antifungal activity of SNPs colloids has indicated that the highest inhibition zone was detected with SNPs. In the case of SNPs synthesized by A. terreus PNU37, the highest Inhibition percentage (IP %) 67.6% at the concentration 150 ppm of SNPs. Results have also indicated that the SNPs synthesized by A. flavus PNU05 at a concentration of 150 ppm/100 ml culture medium gave the highest reduction of B1 determined by HPLC, where the percentages of reduction (PR%) was 56.45%.ISSR analysis revealed a high level of genetic diversity in the Aspergillus spp. population and useful for genetic characterization. ISSR markers were not suitable to discriminate between producing and non-producing SNPs isolates. There was no clear-cut relationship between the ISSR markers (genotype of isolates), antifungal and anti-aflatoxigenic properties.
英文关键词	Green synthesis Aspergillus spp. silver nanoparticles ISSR markers optimization antibacterial activity anti-aflatoxigenic
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000493108500025
WOS关键词	GREEN SYNTHESIS ; EXTRACELLULAR SYNTHESIS ; ANTIBACTERIAL ACTIVITY ; BIOSYNTHESIS ; FLAVUS ; FUNGI ; IDENTIFICATION ; DNA
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
来源机构	King Saud University
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/369683
作者单位	[Zaban, Mayasar Ibrahim; Azim, Nahla Shazli Abdel] Princess Nourah Bint Abdulrahman Univ, Biol Dept, Coll Sci, Riyadh, Saudi Arabia; [Abd El-Aziz, Abeer Ramadan Mohamed] King Saud Univ, Bot & Microbiol Dept, Coll Sci, Riyadh, Saudi Arabia
推荐引用方式 GB/T 7714	Zaban, Mayasar Ibrahim,Azim, Nahla Shazli Abdel,Abd El-Aziz, Abeer Ramadan Mohamed. Antifungal and anti-aflatoxin efficacy of biogenic silver nanoparticles produced by Aspergillus species: Molecular study[J]. King Saud University,2019,32(5):2509-2526.
APA	Zaban, Mayasar Ibrahim,Azim, Nahla Shazli Abdel,&Abd El-Aziz, Abeer Ramadan Mohamed.(2019).Antifungal and anti-aflatoxin efficacy of biogenic silver nanoparticles produced by Aspergillus species: Molecular study.PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES,32(5),2509-2526.
MLA	Zaban, Mayasar Ibrahim,et al."Antifungal and anti-aflatoxin efficacy of biogenic silver nanoparticles produced by Aspergillus species: Molecular study".PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES 32.5(2019):2509-2526.