干旱区生态环境知识资源中心(Arid): High frequency of Aeromonas spp. and virulence genes in Nile Tilapia farms (Oreochromis niloticus) in semi-arid regions of Pernambuco, Brazil

Arid

DOI	10.1590/1678-4162-10747
	High frequency of Aeromonas spp. and virulence genes in Nile Tilapia farms (Oreochromis niloticus) in semi-arid regions of Pernambuco, Brazil
	Kim, F. J. P.; Silva, A. E. M.; Silva, R. V. S.; Kim, P. C. P.; Acosta, A. C.; Silva, S. M. B. C.; Sena, M. J.; Mota, R. A.
通讯作者	Kim, FJP (corresponding author), Inst Fed Educ Ciencia & Tecnol Pernambuco, Barreiros, PE, Brazil.
来源期刊	ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA
ISSN	0102-0935
EISSN	1678-4162
出版年	2019
卷号	71 期号:5 页码:1609-1615
英文摘要	The purpose of this study was to evaluate the occurrence of Aeromonas spp. from fishes and tilapia net-cage farm water in semi-arid regions of Pernambuco and to evaluate the frequency of the aerolysin (aerA), cytotoxic enterotoxin (act), cytotonic enterotoxin (alt) and serine protease (ahp) genes in Aeromonas isolates. 70 live and eight dead fish with aeromoniosis clinical signs and 16 water samples were analyzed. Aeromonas spp. isolated were identified by microbiological (biochemical evidence) and molecular analysis using specific primers for 16SrRNA region, while the distribution of four virulence factors, including aerA, alt, act and ahp, was investigated by PCR assay. One hundred fifty-five (84.7%) isolates were confirmed as Aeromonas spp. by molecular analysis. The most frequent virulence genes in isolates were act (53.55%) and aerA (51,61%). According to the kind of sample, the higher frequency of aerA gene (87.5% P=0.0474) was observed in isolates from dead fish and the lowest frequency of act gene (47.73% P=0.0002) from live fish. This study found the presence of aeromoniosis on tilapia farming in net-cages on Jatoba and Petrolandia counties in the semiarid Pernambuco region. The detection of aerA, act and alt can be used for virulence typing of Aeromonas spp. isolates.
英文关键词	aeromoniosis virulence factors Oreochromis niloticus PCR
类型	Article
语种	葡萄牙语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000493391100026
WOS关键词	FISH PATHOGENS ; HYDROPHILA ; BACTERIAL ; PCR
WOS类目	Veterinary Sciences
WOS研究方向	Veterinary Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/369669
作者单位	[Kim, F. J. P.] Inst Fed Educ Ciencia & Tecnol Pernambuco, Barreiros, PE, Brazil; [Silva, A. E. M.; Silva, R. V. S.; Kim, P. C. P.; Acosta, A. C.; Silva, S. M. B. C.; Sena, M. J.; Mota, R. A.] Univ Fed Rural Pernambuco, Recife, PE, Brazil
推荐引用方式 GB/T 7714	Kim, F. J. P.,Silva, A. E. M.,Silva, R. V. S.,et al. High frequency of Aeromonas spp. and virulence genes in Nile Tilapia farms (Oreochromis niloticus) in semi-arid regions of Pernambuco, Brazil[J],2019,71(5):1609-1615.
APA	Kim, F. J. P..,Silva, A. E. M..,Silva, R. V. S..,Kim, P. C. P..,Acosta, A. C..,...&Mota, R. A..(2019).High frequency of Aeromonas spp. and virulence genes in Nile Tilapia farms (Oreochromis niloticus) in semi-arid regions of Pernambuco, Brazil.ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA,71(5),1609-1615.
MLA	Kim, F. J. P.,et al."High frequency of Aeromonas spp. and virulence genes in Nile Tilapia farms (Oreochromis niloticus) in semi-arid regions of Pernambuco, Brazil".ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA 71.5(2019):1609-1615.