干旱区生态环境知识资源中心(Arid): Diversity of Azotobacter in relation to soil environment in Ebinur Lake wetland

Arid

DOI	10.1080/13102818.2019.1659181
	Diversity of Azotobacter in relation to soil environment in Ebinur Lake wetland
	Zhong Zhengtao; Hu Wenge; Yang Di; He Yuan; Zhou Tingting
通讯作者	Hu, WG (corresponding author), Shihezi Univ, Coll Life Sci, Shihezi, Peoples R China.
来源期刊	BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
ISSN	1310-2818
EISSN	1314-3530
出版年	2019
卷号	33 期号:1 页码:1280-1290
英文摘要	Ebinur Lake wetland is the most representative temperate arid zone wetland ecosystem in China and it is the centre of the oasis and desertification of the Northern slope of Tianshan conjugate. Soil samples were collected from three sites (Tamarix ramosissima, Halocnemum strobilaceum, and Phragmites australis) and different soil layers (0-5, 5-15,15-25, and 25-35 cm) within this wetland during spring, summer and autumn, and the diversity of cultured/uncultured Azotobacter based on the 16S rDNA and nifH genes was characterized. Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) and bivariate correlation analysis were used to evaluate the relationship between the diversity of uncultured Azotobacter and soil environmental factors. A total of 17 cultured Azotobacter strains were isolated, five of which had stronger nitrogen-fixation ability (more than 10 mg/mL). Meanwhile, most of the cultured Azotobacter strain sequences were grouped into Bacillus sphaericus and Bacillus subtilis, and fewer sequences were affiliated with Actinomycetes. Furthermore, PCR-DGGE indicated that the diversity of uncultured Azotobacter was not balanced in some samples and the Shannon diversity index varied from 0.685 to 2.626. The uncultured Azotobacter were grouped into autotrophic genera Azotobacter and Rhizobium. Finally, bivariate correlation analysis showed that the diversity of uncultured Azotobacter was significantly correlated with soil organic carbon, inorganic carbon, NH4+-N, NO3--N and microbial biomass N in the Ebinur Lake wetland in Xinjiang.
英文关键词	Azotobacter PCR-DGGE 16S rRNA gene nifH gene Ebinur Lake wetland
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000505268100004
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/369653
作者单位	[Zhong Zhengtao; Hu Wenge; Yang Di; He Yuan; Zhou Tingting] Shihezi Univ, Coll Life Sci, Shihezi, Peoples R China
推荐引用方式 GB/T 7714	Zhong Zhengtao,Hu Wenge,Yang Di,et al. Diversity of Azotobacter in relation to soil environment in Ebinur Lake wetland[J],2019,33(1):1280-1290.
APA	Zhong Zhengtao,Hu Wenge,Yang Di,He Yuan,&Zhou Tingting.(2019).Diversity of Azotobacter in relation to soil environment in Ebinur Lake wetland.BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,33(1),1280-1290.
MLA	Zhong Zhengtao,et al."Diversity of Azotobacter in relation to soil environment in Ebinur Lake wetland".BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT 33.1(2019):1280-1290.