干旱区生态环境知识资源中心(Arid): Associative diazotrophic bacteria from forage grasses in the Brazilian semi-arid region are effective plant growth promoters

Arid

DOI	10.1071/CP19076
	Associative diazotrophic bacteria from forage grasses in the Brazilian semi-arid region are effective plant growth promoters
	Antunes, Gabiane dos Reis; Assis Santana, Sheilla Rios; Costa Escobar, Indra Elena; Brasil, Marivaine da Silva; Leal de Araujo, Gherman Garcia; Voltolini, Tadeu Vinhas; Fernandes-Junior, Paulo Ivan
通讯作者	Fernandes, PI (corresponding author), Embrapa Semiarido, BR-56302970 Petrolina, PE, Brazil.
来源期刊	CROP & PASTURE SCIENCE
ISSN	1836-0947
EISSN	1836-5795
出版年	2019
卷号	70 期号:10 页码:899-907
英文摘要	The study of plant growth-promoting bacteria (PGPB) can identify outstanding bacteria for crops. For forage grasses adapted to drylands, the selection of PGPB can increase the field performance of pastures. The aim of this study was to isolate, and characterise at molecular, biochemical and symbiotic levels, diazotrophic bacteria obtained from buffel grass (Cenchrus ciliaris), sorghum (Sorghum bicolor) and Tifton 85 (Cynodon spp.) from Brazilian semi-arid region fields. Field-grown plants were collected, and the roots were surface-disinfected, crushed and inoculated in a semi-solid medium. After the formation and confirmation of microaerophilic pellicles, the bacteria were isolated and purified. All bacterial isolates were subjected to nifH gene amplification and identified by their partial 16S rRNA gene sequences. The bacteria were evaluated for the production of auxins and siderophores, calcium phosphate solubilisation, and diazotrophic ability as 'in vitro' plant growth-promotion traits. A plant inoculation assay was conducted to assess the plant growth-promotion abilities of the bacterial isolates. Twenty-one bacterial isolates harboured the nifH gene (nifH(+)), among which nine were obtained from sorghum, eight from buffel grass, and four from Tifton 85. The bacterial isolates were classified as Bacillus (8), Stenotrophomonas (7), Agrobacterium (4), Cellulomonas (1) and Paenibacillus (1). All were shown to be auxin producers, with 14 isolates showing diazotrophic capacity 'in vitro'. Fourteen isolates increased plant N content, but the bacterial strains ESA 392 and ESA 398 (Bacillus), ESA 397 and ESA 407 (Stenotrophomonas), and ESA 401 (Agrobacterium) were shown to promote both plant growth and N nutrition. These strains are candidates for further assays to evaluate their agronomic performance under field conditions, aiming inoculant production for forage grasses in drylands.
英文关键词	biological nitrogen fixation Caatinga plant growth promotion traits strain selection
类型	Article
语种	英语
开放获取类型	Green Published
收录类别	SCI-E
WOS记录号	WOS:000493548800008
WOS关键词	NITROGEN-FIXATION ; DIVERSITY ; RHIZOBACTERIA ; INOCULATION ; SPP. ; BURKHOLDERIA ; RHIZOSPHERE ; SELECTION ; POACEAE ; FIXERS
WOS类目	Agriculture, Multidisciplinary
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/369626
作者单位	[Antunes, Gabiane dos Reis; Assis Santana, Sheilla Rios] Univ Fed Vale Sao Francisco, Colegiado Zootecnia, BR-56300000 Petrolina, PE, Brazil; [Costa Escobar, Indra Elena] Univ Fed Vale Sao Francisco, Colegiado Farm, BR-56304917 Petrolina, PE, Brazil; [Brasil, Marivaine da Silva] Univ Fed Mato Grosso do Sul, Campus Pantanal, BR-79304902 Corumba, MS, Brazil; [Leal de Araujo, Gherman Garcia; Voltolini, Tadeu Vinhas; Fernandes-Junior, Paulo Ivan] Embrapa Semiarido, BR-56302970 Petrolina, PE, Brazil
推荐引用方式 GB/T 7714	Antunes, Gabiane dos Reis,Assis Santana, Sheilla Rios,Costa Escobar, Indra Elena,et al. Associative diazotrophic bacteria from forage grasses in the Brazilian semi-arid region are effective plant growth promoters[J],2019,70(10):899-907.
APA	Antunes, Gabiane dos Reis.,Assis Santana, Sheilla Rios.,Costa Escobar, Indra Elena.,Brasil, Marivaine da Silva.,Leal de Araujo, Gherman Garcia.,...&Fernandes-Junior, Paulo Ivan.(2019).Associative diazotrophic bacteria from forage grasses in the Brazilian semi-arid region are effective plant growth promoters.CROP & PASTURE SCIENCE,70(10),899-907.
MLA	Antunes, Gabiane dos Reis,et al."Associative diazotrophic bacteria from forage grasses in the Brazilian semi-arid region are effective plant growth promoters".CROP & PASTURE SCIENCE 70.10(2019):899-907.