干旱区生态环境知识资源中心(Arid): Identification of differentially expressed genes of Haloxylon ammodendron in response to salinity stress

Arid

DOI	10.32615/bp.2019.066
	Identification of differentially expressed genes of Haloxylon ammodendron in response to salinity stress
	He, J. F.; Fang, Y. Y.; Lu, Z. X.; Wang, L. W.; Zhao, X. Q.; Fu, X. F.; Zhao, J.; Liu, H. K.
通讯作者	Liu, HK (corresponding author), Inner Mongolia Acad Agr & Anim Husb Sci, Biotechnol Res Ctr, Hohhot 010030, Peoples R China. ; Zhao, J (corresponding author), Inner Mongolia Med Univ, Affiliated Hosp, Reprod Ctr, Hohhot 010050, Peoples R China.
来源期刊	BIOLOGIA PLANTARUM
ISSN	0006-3134
EISSN	1573-8264
出版年	2019
卷号	63 页码:610-617
英文摘要	Haloxylon ammodendron (C.A. Mey.), an endangered desert tree with excellent drought and salinity tolerance, provides a unique genotype to characterize and understand the tolerance mechanisms. In this study, four RNA-Seq libraries were constructed and sequenced from H. ammodendron under salinity stress. Total 12 027 differentially expressed genes (DEGs) were identified, in which 4 023, 3 517, 4 487 genes were differentially expressed under light salinity stress (200 mM NaCl), moderate salinity stress (400 mM NaCl), and severe salinity stress (800 mM NaCl), respectively. The up-regulated DEGs included several transcription factors (e.g., MYB and bHLH), hormone-related genes (e.g., cytochrome P450), protein kinases (e.g., Atpk2-Atpk19 like), and genes involved in carbon metabolism (e.g., UDP glycosyltransferase), osmotic regulation (e.g., proline transporter), and ubiquitin proteasome system (e.g., ubiquitin-conjugating enzymes). Heat shock proteins were identified as positive regulators of salinity tolerance in H. ammodendron. The expression patterns of 13 DEGs verified by real-time quantitative PCR were identically consistent with the variations in transcript abundance identified by RNA-Seq. Our results provide new insights into molecular mechanism of H. ammodendron in response to salinity stress.
英文关键词	carbon metabolism drought and salinity tolerance heat shock proteins hormone-related genes NaCl RNA-Seq transcription factors
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000506616900004
WOS关键词	IMPROVES DROUGHT TOLERANCE ; NAC TRANSCRIPTION FACTORS ; SALT STRESS ; PROTEIN-KINASE ; RESISTANCE ; PROFILES ; RICE
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/369583
作者单位	[He, J. F.; Fang, Y. Y.; Wang, L. W.; Zhao, X. Q.; Fu, X. F.; Liu, H. K.] Inner Mongolia Acad Agr & Anim Husb Sci, Biotechnol Res Ctr, Hohhot 010030, Peoples R China; [Zhao, J.] Inner Mongolia Med Univ, Affiliated Hosp, Reprod Ctr, Hohhot 010050, Peoples R China; [Lu, Z. X.] Agr & Agri Food Canada, Lethbridge Res Ctr, Lethbridge, AB T1J 4B1, Canada
推荐引用方式 GB/T 7714	He, J. F.,Fang, Y. Y.,Lu, Z. X.,et al. Identification of differentially expressed genes of Haloxylon ammodendron in response to salinity stress[J],2019,63:610-617.
APA	He, J. F..,Fang, Y. Y..,Lu, Z. X..,Wang, L. W..,Zhao, X. Q..,...&Liu, H. K..(2019).Identification of differentially expressed genes of Haloxylon ammodendron in response to salinity stress.BIOLOGIA PLANTARUM,63,610-617.
MLA	He, J. F.,et al."Identification of differentially expressed genes of Haloxylon ammodendron in response to salinity stress".BIOLOGIA PLANTARUM 63(2019):610-617.