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DOI	10.1007/s00344-019-10056-5
	Differential Gene Expression Pattern of Drought Responsive Transcription Factors in Chickpea: An Expressional Analysis
	Borhani, Sara; Vessal, Saeedreza; Bagheri, Abdolreza; Shokouhifar, Farhad
通讯作者	Vessal, S (corresponding author), Ferdowsi Univ Mashhad, Res Ctr Plant Sci, Mashhad, Razavi Khorasan, Iran.
来源期刊	JOURNAL OF PLANT GROWTH REGULATION
ISSN	0721-7595
EISSN	1435-8107
出版年	2020
卷号	39 期号:3 页码:1211-1220
英文摘要	Chickpea (Cicer arietinumL.) is an important grain crop mainly grown in arid and semi-arid regions of the world. Drought is the major factor limiting chickpea growth and productivity. Transcription factors (TFs) genes have been reported as key regulators of drought tolerance in plants. In this study, we determined the relative gene expression of transcription factors ofWRKY,DREB2A, andCarNAC3in three weeks seedlings of tolerant (MCC537) and susceptible (MCC674) genotypes under progressive water deficit through semi-quantitative reverse transcriptase PCR (SqRT-PCR) method. Furthermore, results of SqRT-PCR were more confirmed by quantitative PCR (qPCR). Relative gene expression analysis of the selected genes using qPCR revealed different dehydration-responsive expression patterns. The expression ofWRKYgene was significantly induced in both tolerant and sensitive genotypes under severe drought stress (at the last time point). Also, in tolerant genotype,DREB2Agene was approximately expressed three, five and fourfold higher than control plants at three times points, respectively. Moreover, the expression level ofCarNAC3gene in this genotype increased four and twofold higher compared to the relative control at 2 and 4 days after stress, respectively. However, the expression of these genes in the susceptible genotype was constant or decreased relative to the control. Furthermore, theCarNAC3andDREB2Agenes showed higher and more effective expression than theWRKYgene. Overall, the results demonstrated that these TFs may play an important role to improve drought tolerance and have a potential to facilitate molecular breeding and development of drought-tolerant chickpea varieties.
英文关键词	Drought Chickpea Transcription factors Gene expression qPCR
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000541588900001
WOS关键词	CICER-ARIETINUM L. ; COLD-RESPONSE ; SALT STRESS ; TOLERANCE ; OVEREXPRESSION ; PROFILES ; WHEAT ; SUPERFAMILY ; GENOTYPES ; DREB2A
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/369259
作者单位	[Borhani, Sara; Bagheri, Abdolreza] Ferdowsi Univ Mashhad, Coll Agr, Biotechnol & Plant Breeding Dept, Mashhad, Razavi Khorasan, Iran; [Vessal, Saeedreza; Shokouhifar, Farhad] Ferdowsi Univ Mashhad, Res Ctr Plant Sci, Mashhad, Razavi Khorasan, Iran
推荐引用方式 GB/T 7714	Borhani, Sara,Vessal, Saeedreza,Bagheri, Abdolreza,et al. Differential Gene Expression Pattern of Drought Responsive Transcription Factors in Chickpea: An Expressional Analysis[J],2020,39(3):1211-1220.
APA	Borhani, Sara,Vessal, Saeedreza,Bagheri, Abdolreza,&Shokouhifar, Farhad.(2020).Differential Gene Expression Pattern of Drought Responsive Transcription Factors in Chickpea: An Expressional Analysis.JOURNAL OF PLANT GROWTH REGULATION,39(3),1211-1220.
MLA	Borhani, Sara,et al."Differential Gene Expression Pattern of Drought Responsive Transcription Factors in Chickpea: An Expressional Analysis".JOURNAL OF PLANT GROWTH REGULATION 39.3(2020):1211-1220.