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DOI	10.4103/pm.pm_199_20
	Terfezia claveryi and Terfezia boudieri extracts: An antimicrobial and molecular assay on clinical isolates associated with eye infections
	Badger-Emeka, Lorina Ineta; Emeka, Promise Madu; Aldossari, Saif; Khalil, Hany Ezzat
通讯作者	Badger-Emeka, LI (corresponding author), King Faisal Univ, Coll Med, Dept Biomed Sci, Al Hasa 31982, Saudi Arabia.
来源期刊	PHARMACOGNOSY MAGAZINE
ISSN	0973-1296
EISSN	0976-4062
出版年	2020
卷号	16 期号:72 页码:780-788
英文摘要	Background: Ocular infections are capable of spreading to different anatomical sites of the eyes and, if not appropriately treated, can lead to blindness. The emergence of difficult to treat microbial infections has led to the search of alternatives from natural sources. Objectives: The antimicrobial effects of Terfezia claveryi and Terfezia boudieri (T. boudieri) against bacteria isolates associated with eye infections and their molecular mechanism were investigated. Materials and Methods: Crude aqueous and methanolic extracts, including fractions of chloroform, petroleum, and ethyl acetate of T. claveryi and T. boudieri, were used for the investigation. Bacterial isolation and identification were carried out using basic microbiological and biochemical techniques. scanning electron microscopy.(SEM) and molecular docking were used to adduce possible antimicrobial mechanism of these extracts and their fractions. Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus lugdunensis, Serratia odorifera, Serratia liquefaciens, Pseudomonas stutzeri, Pseudomonas oryzihabitans, Proteus mirabililis, Kocuria kristinae, Kocuria rosea, and Micrococcus luteus were isolated from patients with ocular infections. Results: Isolates were resistant to benzylpenicillin (78.0%), rifampicin (57.0%), tetracycline (56.0%), clindamycin (33.3%), and tigecycline (24.0%). Furthermore, the percentage resistance to gentamicin and ciprofloxacin was 13.0% each. All isolates were susceptible to extracts/fractions of T. claveryi and T. boudieri. Docking analysis showed binding with surface protein Sortase A of Staphylococcus aureus, indicating that stigmasterol, the active compound in both Terfezia species, interacted with valine amino acid 110. SEM imaging showed morphological alterations in treated isolated Staphylococcal species. Conclusion: Therefore, extracts of both Terfezia species have demonstrated the potential to possess antibacterial activity, which can be further exploited for clinical use.
英文关键词	Antimicrobials extracts isolates ocular infections Terfezia boudieri Terfezia claveryi
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000621667300015
WOS关键词	ANTIBIOTIC-RESISTANCE ; STAPHYLOCOCCUS-AUREUS ; OCULAR INFECTIONS ; PSEUDOMONAS-AERUGINOSA ; CHEMICAL-COMPOSITION ; DESERT TRUFFLES ; ANTIOXIDANT ; SUSCEPTIBILITIES ; KERATITIS ; CULTURE
WOS类目	Chemistry, Medicinal
WOS研究方向	Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/369133
作者单位	[Badger-Emeka, Lorina Ineta] King Faisal Univ, Coll Med, Dept Biomed Sci, Al Hasa 31982, Saudi Arabia; [Emeka, Promise Madu; Khalil, Hany Ezzat] King Faisal Univ, Coll Clin Pharm, Dept Pharmaceut Sci, Al Hasa, Saudi Arabia; [Aldossari, Saif] King Faisal Univ, Coll Med, Dept Ophthalmol, Al Hasa, Saudi Arabia; [Khalil, Hany Ezzat] Minia Univ, Fac Pharm, Dept Pharmacognosy, Al Minya 61519, Egypt
推荐引用方式 GB/T 7714	Badger-Emeka, Lorina Ineta,Emeka, Promise Madu,Aldossari, Saif,et al. Terfezia claveryi and Terfezia boudieri extracts: An antimicrobial and molecular assay on clinical isolates associated with eye infections[J],2020,16(72):780-788.
APA	Badger-Emeka, Lorina Ineta,Emeka, Promise Madu,Aldossari, Saif,&Khalil, Hany Ezzat.(2020).Terfezia claveryi and Terfezia boudieri extracts: An antimicrobial and molecular assay on clinical isolates associated with eye infections.PHARMACOGNOSY MAGAZINE,16(72),780-788.
MLA	Badger-Emeka, Lorina Ineta,et al."Terfezia claveryi and Terfezia boudieri extracts: An antimicrobial and molecular assay on clinical isolates associated with eye infections".PHARMACOGNOSY MAGAZINE 16.72(2020):780-788.