干旱区生态环境知识资源中心(Arid): Resuscitation of soil microbiota after

Arid

DOI	10.1016/j.ejsobi.2021.103290
	Resuscitation of soil microbiota after > 70-years of desiccation
	Zhao, Jun; Chen, Dongfeng; Gao, Wei; Guo, Zhiying; Jia, Zhongjun; Hernandez, Marcela
通讯作者	Chen, DF (corresponding author), Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Jiangsu, Peoples R China. ; Hernandez, M (corresponding author), Univ East Anglia, Sch Environm Sci, Norwich Res Pk, Norwich, Norfolk, England.
来源期刊	EUROPEAN JOURNAL OF SOIL BIOLOGY
ISSN	1164-5563
EISSN	1778-3615
出版年	2021
卷号	103
英文摘要	The response pattern of soil bacterial communities to drought events has been characterized from short-term laboratory desiccation and in naturally arid soils, but the resistance of soil bacteria to persistently long periods of desiccation remains largely unknown. This study assessed the abundance and composition of bacteria in 24 historical soils air-dried for more than 70 years, by quantification and high-throughput sequencing analyses of 16S rRNA genes. All soils contained measurable abundances of bacteria varying from 103 to 108 gene copies g(-1) soil and contrasting community compositions were observed in soils with different land histories, suggesting that the detected bacteria were indigenous to the field. A 28-day soil rewetting incubation significantly increased the bacterial gene abundance in soils, indicating some bacteria in these soils were still alive and quickly resuscitated. Among all identified taxa, Paenibacillus, Cohnella and two unclassified Bacillales genera within the phylum Firmicutes showed growth in the highest number of soils (>= 12 soils), whereas Bacillales genera Tumebacillus, Alicyclobacillus and Brevibacillus displayed the strongest growth activity (>1000-fold increase in gene abundance) following rewet. Some Actinobacteria and Proteobacteria genera also showed relatively high activity following the rewet, suggesting that the tolerance to prolonged desiccation is a common trait across phylogenetically divergent microbes. The present study thus confirmed the value of desiccated soils for detecting the composition difference of bacteria, and provided evidence for strong adaptation of some bacteria to long-term desiccation and extreme osmotic change.
英文关键词	Long-term soil desiccation Dry-rewet 16S rRNA gene Quantitative PCR Amplicon sequencing Soil archives
类型	Article
语种	英语
开放获取类型	Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000633041200003
WOS关键词	ARCHIVED SOILS ; COMMUNITIES ; UK ; BACTERIAL ; SURVIVAL ; DESERT ; MICROORGANISMS ; CONTAMINATION ; DIVERSITY ; SAMPLES
WOS类目	Ecology ; Soil Science
WOS研究方向	Environmental Sciences & Ecology ; Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/368972
作者单位	[Zhao, Jun; Chen, Dongfeng; Gao, Wei; Guo, Zhiying; Jia, Zhongjun] Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Jiangsu, Peoples R China; [Gao, Wei; Guo, Zhiying] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Hernandez, Marcela] Univ East Anglia, Sch Environm Sci, Norwich Res Pk, Norwich, Norfolk, England
推荐引用方式 GB/T 7714	Zhao, Jun,Chen, Dongfeng,Gao, Wei,et al. Resuscitation of soil microbiota after > 70-years of desiccation[J],2021,103.
APA	Zhao, Jun,Chen, Dongfeng,Gao, Wei,Guo, Zhiying,Jia, Zhongjun,&Hernandez, Marcela.(2021).Resuscitation of soil microbiota after > 70-years of desiccation.EUROPEAN JOURNAL OF SOIL BIOLOGY,103.
MLA	Zhao, Jun,et al."Resuscitation of soil microbiota after > 70-years of desiccation".EUROPEAN JOURNAL OF SOIL BIOLOGY 103(2021).