Knowledge Resource Center for Ecological Environment in Arid Area
| DOI | 10.1016/j.plantsci.2020.110748 |
| Transcriptome-based metabolic profiling of flavonoids in Agave lechuguilla waste biomass | |
| Morreeuw, Zoe P.; Escobedo-Fregoso, Cristina; Rios-Gonzalez, Leopoldo J.; Castillo-Quiroz, David; Reyes, Ana G. | |
| 通讯作者 | Reyes, AG (corresponding author), Ave Inst Politecn Nacl 195, La Paz 23096, Bcs, Mexico. |
| 来源期刊 | PLANT SCIENCE
 |
| ISSN | 0168-9452 |
| EISSN | 1873-2259 |
| 出版年 | 2021 |
| 卷号 | 305 |
| 英文摘要 | Agave lechuguilla is one of the most abundant species in arid and semiarid regions of Mexico, and is used to extract fiber. However, 85 % of the harvested plant material is discarded. Previous bioprospecting studies of the waste biomass suggest the presence of bioactive compounds, although the extraction process limited metabolite characterization. This work achieved flavonoid profiling of A. lechuguilla in both processed and non-processed leaf tissues using transcriptomic analysis. Functional annotation of the first de novo transcriptome of A. lechuguilla (255.7 Mbp) allowed identifying genes coding for 33 enzymes and 8 transcription factors involved in flavonoid biosynthesis. The flavonoid metabolic pathway was mostly elucidated by HPLC-MS/MS screening of alcoholic extracts. Key genes of flavonoid synthesis were higher expressed in processed leaf tissues than in non-processed leaves, suggesting a high content of flavonoids and glycoside derivatives in the waste biomass. Tar-geted HPLC-UV-MS analyses confirmed the concentration of isorhamnetin (1251.96 ?g), flavanone (291.51 ?g), hesperidin (34.23 ?g), delphinidin (24.23 ?g), quercetin (15.57 ?g), kaempferol (13.71 ?g), cyanidin (12.32 ?g), apigenin (9.70 ?g) and catechin (7.91 ?g) per gram of dry residue. Transcriptomic and biochemical profiling concur in the potential of lechuguilla by-products with a wide range of applications in agriculture, feed, food, cosmetics, and pharmaceutical industries. |
| 英文关键词 | Agave lechuguilla Waste biomass Flavonoids biosynthesis Transcriptomic analysis Metabolite profiling HPLC analysis |
| 类型 | Article |
| 语种 | 英语 |
| 收录类别 | SCI-E |
| WOS记录号 | WOS:000632529100007 |
| WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| 资源类型 | 期刊论文 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/368913 |
| 作者单位 | [Morreeuw, Zoe P.] Ctr Invest Biol Noroeste CIBNOR, Ave Inst Politecn Nacl 195, La Paz 23096, Bcs, Mexico; [Escobedo-Fregoso, Cristina; Reyes, Ana G.] CONACYTCIBNOR, Ave Inst Politecn Nacl 195, La Paz 23096, Bcs, Mexico; [Rios-Gonzalez, Leopoldo J.] Univ Autonoma Coahuila UAdeC, Fac Ciencias Quim, Dept Biotecnol, Blvd V Carranza, Saltillo 25280, Coahuila, Mexico; [Castillo-Quiroz, David] Inst Nacl Invest Forest Agr & Pecuarias INIFAP, Carretera Saltillo Zacatecas 9515, Saltillo 25315, Coahuila, Mexico |
| 推荐引用方式 GB/T 7714 | Morreeuw, Zoe P.,Escobedo-Fregoso, Cristina,Rios-Gonzalez, Leopoldo J.,et al. Transcriptome-based metabolic profiling of flavonoids in Agave lechuguilla waste biomass[J],2021,305. |
| APA | Morreeuw, Zoe P.,Escobedo-Fregoso, Cristina,Rios-Gonzalez, Leopoldo J.,Castillo-Quiroz, David,&Reyes, Ana G..(2021).Transcriptome-based metabolic profiling of flavonoids in Agave lechuguilla waste biomass.PLANT SCIENCE,305. |
| MLA | Morreeuw, Zoe P.,et al."Transcriptome-based metabolic profiling of flavonoids in Agave lechuguilla waste biomass".PLANT SCIENCE 305(2021). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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