干旱区生态环境知识资源中心(Arid): Species diversity and spatial distribution of CL/VL vectors: assessing bioclimatic effect on expression plasticity of genes possessing vaccine properties isolated from wild-collected sand flies in endemic areas of Iran

Arid

DOI	10.1186/s12879-021-06129-0
	Species diversity and spatial distribution of CL/VL vectors: assessing bioclimatic effect on expression plasticity of genes possessing vaccine properties isolated from wild-collected sand flies in endemic areas of Iran
	Bordbar, Ali; Parvizi, Parviz
通讯作者	Parvizi, P (corresponding author), Pasteur Inst, Parasitol Dept, Mol Systemat Lab, 69 Pasteur Ave, Tehran, Iran.
来源期刊	BMC INFECTIOUS DISEASES
EISSN	1471-2334
出版年	2021
卷号	21 期号:1
英文摘要	BackgroundLeishmaniasis is one of the ten most important neglected tropical diseases worldwide. Understanding the distribution of vectors of visceral and cutaneous leishmaniasis (VL/CL) is one of the significant strategic frameworks to control leishmaniasis. In this study, the extent of the bioclimatic variability was investigated to recognize a rigorous cartographic of the spatial distribution of VL/CL vectors as risk-maps using ArcGIS modeling system. Moreover, the effect of bioclimatic diversity on the fold change expression of genes possessing vaccine traits (SP15 and LeIF) was evaluated in each bioclimatic region using real-time PCR analysis.MethodsThe Inverse Distance Weighting interpolation method was used to obtain accurate geography map in closely-related distances. Bioclimatic indices were computed and vectors spatial distribution was analyzed in ArcGIS10.3.1 system. Species biodiversity was calculated based on Shannon diversity index using Rv.3.5.3. Expression fold change of SP15 and LeIF genes was evaluated using cDNA synthesis and RT-qPCR analysis.ResultsFrequency of Phlebotomus papatasi was predominant in plains areas of Mountainous bioclimate covering the CL hot spots. Mediterranean region was recognized as an important bioclimate harboring prevalent patterns of VL vectors. Semi-arid bioclimate was identified as a major contributing factor to up-regulate salivary-SP15 gene expression (P=0.0050, P<0.05). Also, Mediterranean bioclimate had considerable effect on up-regulation of Leishmania-LeIF gene in gravid and semi-gravid P. papatasi population (P=0.0109, P<0.05).ConclusionsThe diversity and spatial distribution of CL/VL vectors associated with bioclimatic regionalization obtained in our research provide epidemiological risk maps and establish more effectively control measures against leishmaniasis. Oscillations in gene expression indicate that each gene has its own features, which are profoundly affected by bioclimatic characteristics and physiological status of sand flies. Given the efficacy of species-specific antigens for vaccine production, it is essential to consider bioclimatic factors that have a fundamental role in affecting the regulatory regions of environmentally responsive loci for genes used in vaccine design.
英文关键词	Leishmaniasis CL and VL vectors Spatial distribution Bioclimatic effect Genes with vaccine traits GIS modeling
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000658359900005
WOS关键词	PHLEBOTOMUS-PAPATASI DIPTERA ; LEISHMANIA-MAJOR ; CUTANEOUS LEISHMANIASIS ; LUTZOMYIA-LONGIPALPIS ; KHORASAN PROVINCE ; SALIVARY PROTEINS ; ECOLOGICAL NICHE ; FLY ; PSYCHODIDAE ; TRANSMISSION
WOS类目	Infectious Diseases
WOS研究方向	Infectious Diseases
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/368769
作者单位	[Bordbar, Ali; Parvizi, Parviz] Pasteur Inst, Parasitol Dept, Mol Systemat Lab, 69 Pasteur Ave, Tehran, Iran
推荐引用方式 GB/T 7714	Bordbar, Ali,Parvizi, Parviz. Species diversity and spatial distribution of CL/VL vectors: assessing bioclimatic effect on expression plasticity of genes possessing vaccine properties isolated from wild-collected sand flies in endemic areas of Iran[J],2021,21(1).
APA	Bordbar, Ali,&Parvizi, Parviz.(2021).Species diversity and spatial distribution of CL/VL vectors: assessing bioclimatic effect on expression plasticity of genes possessing vaccine properties isolated from wild-collected sand flies in endemic areas of Iran.BMC INFECTIOUS DISEASES,21(1).
MLA	Bordbar, Ali,et al."Species diversity and spatial distribution of CL/VL vectors: assessing bioclimatic effect on expression plasticity of genes possessing vaccine properties isolated from wild-collected sand flies in endemic areas of Iran".BMC INFECTIOUS DISEASES 21.1(2021).