干旱区生态环境知识资源中心(Arid): Identifying Anti-Oxidant Biosynthesis Genes in Pearl Millet [Pennisetum glaucum (L.) R. Br.] Using Genome-Wide Association Analysis

Arid

DOI	10.3389/fpls.2021.599649
	Identifying Anti-Oxidant Biosynthesis Genes in Pearl Millet [Pennisetum glaucum (L.) R. Br.] Using Genome-Wide Association Analysis
	Yadav, Chandra Bhan; Tokas, Jayanti; Yadav, Devvart; Winters, Ana; Singh, Ram B.; Yadav, Rama; Gangashetty, Prakash I.; Srivastava, Rakesh K.; Yadav, Rattan S.
通讯作者	Yadav, RS (corresponding author), Aberystwyth Univ, Inst Biol Environm & Rural Sci IBERS, Aberystwyth, Dyfed, Wales.
来源期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
出版年	2021
卷号	12
英文摘要	Pearl millet [Pennisetum glaucum (L.) R Br.] is an important staple food crop in the semi-arid tropics of Asia and Africa. It is a cereal grain that has the prospect to be used as a substitute for wheat flour for celiac patients. It is an important antioxidant food resource present with a wide range of phenolic compounds that are good sources of natural antioxidants. The present study aimed to identify the total antioxidant content of pearl millet flour and apply it to evaluate the antioxidant activity of its 222 genotypes drawn randomly from the pearl millet inbred germplasm association panel (PMiGAP), a world diversity panel of this crop. The total phenolic content (TPC) significantly correlated with DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity (% inhibition), which ranged from 2.32 to 112.45% and ferric-reducing antioxidant power (FRAP) activity ranging from 21.68 to 179.66 (mg ascorbic acid eq./100 g). Genome-wide association studies (GWAS) were conducted using 222 diverse accessions and 67 K SNPs distributed across all the seven pearl millet chromosomes. Approximately, 218 SNPs were found to be strongly associated with DPPH and FRAP activity at high confidence [-log (p) > 3.0-7.4]. Furthermore, flanking regions of significantly associated SNPs were explored for candidate gene harvesting. This identified 18 candidate genes related to antioxidant pathway genes (flavanone 7-O-beta-glycosyltransferase, GDSL esterase/lipase, glutathione S-transferase) residing within or near the association signal that can be selected for further functional characterization. Patterns of genetic variability and the associated genes reported in this study are useful findings, which would need further validation before their utilization in molecular breeding for high antioxidant-containing pearl millet cultivars.
英文关键词	pearl millet [Pennisetum glaucum (L ) R Br ] antioxidant activity phenolics germplasm genome-wide association study marker-trait associations candidate genes
类型	Article
语种	英语
开放获取类型	Green Accepted, Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000660083000001
WOS关键词	PHENOLIC-COMPOUNDS ; LINKAGE DISEQUILIBRIUM ; DIETARY ANTIOXIDANTS ; COMPLEX TRAITS ; POLYPHENOLS ; GRAINS ; HEALTH ; IDENTIFICATION ; POPULATION ; SOFTWARE
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
来源机构	International Crops Research Institute for the Semi-Arid Tropics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/368761
作者单位	[Yadav, Chandra Bhan; Tokas, Jayanti; Yadav, Devvart; Winters, Ana; Yadav, Rama; Yadav, Rattan S.] Aberystwyth Univ, Inst Biol Environm & Rural Sci IBERS, Aberystwyth, Dyfed, Wales; [Tokas, Jayanti; Yadav, Devvart] Haryana Agr Univ, Chaudhary Charan Singh CCS, Hisar, Haryana, India; [Singh, Ram B.; Srivastava, Rakesh K.] Int Crops Res Inst Semi Arid Trop, Patancheru, Andhra Pradesh, India; [Gangashetty, Prakash I.] Int Crops Res Inst Semi Arid Trop, Niamey, Niger
推荐引用方式 GB/T 7714	Yadav, Chandra Bhan,Tokas, Jayanti,Yadav, Devvart,et al. Identifying Anti-Oxidant Biosynthesis Genes in Pearl Millet [Pennisetum glaucum (L.) R. Br.] Using Genome-Wide Association Analysis[J]. International Crops Research Institute for the Semi-Arid Tropics,2021,12.
APA	Yadav, Chandra Bhan.,Tokas, Jayanti.,Yadav, Devvart.,Winters, Ana.,Singh, Ram B..,...&Yadav, Rattan S..(2021).Identifying Anti-Oxidant Biosynthesis Genes in Pearl Millet [Pennisetum glaucum (L.) R. Br.] Using Genome-Wide Association Analysis.FRONTIERS IN PLANT SCIENCE,12.
MLA	Yadav, Chandra Bhan,et al."Identifying Anti-Oxidant Biosynthesis Genes in Pearl Millet [Pennisetum glaucum (L.) R. Br.] Using Genome-Wide Association Analysis".FRONTIERS IN PLANT SCIENCE 12(2021).