干旱区生态环境知识资源中心(Arid): First Report of Antimicrobial Susceptibility and Virulence Gene Characterization Associated with Staphylococcus aureus Carriage in Healthy Camels from Tunisia

Arid

DOI	10.3390/ani11092754
	First Report of Antimicrobial Susceptibility and Virulence Gene Characterization Associated with Staphylococcus aureus Carriage in Healthy Camels from Tunisia
	Ben Chehida, Faten; Gharsa, Haythem; Tombari, Wafa; Selmi, Rachid; Khaldi, Sana; Daaloul, Monia; Ben Slama, Karim; Messadi, Lilia
通讯作者	Ben Chehida, F (corresponding author), Univ Manouba, Natl Sch Vet Med Sidi Thabet, Inst Agr Res & Higher Educ, Lab Microbiol Immunol & Gen Pathol, Sidi Thabet 2020, Tunisia.
来源期刊	ANIMALS
ISSN	2076-2615
出版年	2021
卷号	11 期号:9
英文摘要	Simple Summary The one-humped camel (Camelus dromedarius) is an important livestock species and is present in more than 46 national entities, with 80% of the camel population inhabiting Africa. In these regions, the role of camels in the livestock economy is highly valuable and a part of this camel herd is valorized on national or international markets for meat production, live animal export or milk production. Even if camels are the species that is most adapted to the harsh conditions of arid/semi-arid rangelands, they can be susceptible to a high number of pathogens, including S. aureus. This latter is often associated with asymptomatic carriage but can also be responsible for several diseases, therefore causing considerable economical losses. Continued monitoring and control assume particular importance in containing the spread of the bacterium since it constitutes an important zoonotic disease. A total of 318 nasal and rectal swabs were collected from 159 apparently healthy camels (Camelus dromedarius) randomly selected from five regions in southern and central Tunisia and screened for Staphylococcus aureus carriage. Staphylococcus spp. were recovered from 152 of 159 camels studied (95.6%) and in total 258 swabs (81%) were positive. Among these isolates, 16 were coagulase positive Staphylococcus (CoPS) (6.2%) and were characterized by biochemical and molecular tests as S. aureus. These were isolated from 14 camels (8.8%) with co-carriage in nasal and rectal mucosa by two camels. All S. aureus isolates recovered were methicillin-susceptible Staphylococcus aureus (MSSA) and were characterized by spa typing and PFGE. Three different spa types were recovered: t729, t4013 and a spa type newly registered as t19687, which was the most common. PFGE analysis revealed seven different patterns and these were characterized by MLST, which revealed five different sequence types (ST6, ST88, ST3583 and two new sequences, ST6504 and ST6506). All isolates harbored different virulence genes, including hld, encoding delta hemolysin; lukE-lukD, encoding bicomponent leukotoxin LukE-LukD; the clfB gene, encoding clumping factor B; the laminin gene, encoding laminin-binding protein; and cap8, encoding capsule type 8. Fifteen isolates harbored hemolysin beta (hlb) and fourteen encoded hemolysin alpha (hla) and hemolysin G2 (hlg(v)). Adhesin factors, including clfA and fnbB, were detected in five and four isolates respectively. Binding proteins, including collagen (cbp) and elastin-binding protein (ebp), were detected in two S. aureus isolates while fibrinogen-binding protein (fib) was identified in four isolates. This study provides the first set of genotyping data on the population structure and presence of toxin genes of S. aureus strains in Tunisian camels.
英文关键词	Camelus dromedarius Tunisia Staphylococcus aureus virulence typing antimicrobial resistance
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000699443600001
WOS关键词	METHICILLIN-RESISTANT ; MULTIPLEX-PCR ; NASAL SAMPLES ; COAGULASE ; PSEUDINTERMEDIUS ; EPIDEMIOLOGY ; PREVALENCE ; PATTERNS ; PETS ; FARM
WOS类目	Agriculture, Dairy & Animal Science ; Veterinary Sciences
WOS研究方向	Agriculture ; Veterinary Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/368461
作者单位	[Ben Chehida, Faten; Tombari, Wafa; Selmi, Rachid; Daaloul, Monia; Messadi, Lilia] Univ Manouba, Natl Sch Vet Med Sidi Thabet, Inst Agr Res & Higher Educ, Lab Microbiol Immunol & Gen Pathol, Sidi Thabet 2020, Tunisia; [Gharsa, Haythem; Ben Slama, Karim] Univ Tunis El Manar, Higher Inst Appl Biol Sci Tunis, Lab Microorganisms & Act Biomol, Tunis 2092, Tunisia; [Selmi, Rachid] Minist Natl Def, Vet Serv, Gen Directorate Mil Hlth, Tunis 1008, Tunisia; [Khaldi, Sana] Univ Manouba, Natl Sch Vet Med Sidi Thabet, Inst Agr Res & Higher Educ, Dept Sci & Pathol Anim Reprod, Sidi Thabet 2020, Tunisia
推荐引用方式 GB/T 7714	Ben Chehida, Faten,Gharsa, Haythem,Tombari, Wafa,et al. First Report of Antimicrobial Susceptibility and Virulence Gene Characterization Associated with Staphylococcus aureus Carriage in Healthy Camels from Tunisia[J],2021,11(9).
APA	Ben Chehida, Faten.,Gharsa, Haythem.,Tombari, Wafa.,Selmi, Rachid.,Khaldi, Sana.,...&Messadi, Lilia.(2021).First Report of Antimicrobial Susceptibility and Virulence Gene Characterization Associated with Staphylococcus aureus Carriage in Healthy Camels from Tunisia.ANIMALS,11(9).
MLA	Ben Chehida, Faten,et al."First Report of Antimicrobial Susceptibility and Virulence Gene Characterization Associated with Staphylococcus aureus Carriage in Healthy Camels from Tunisia".ANIMALS 11.9(2021).