Knowledge Resource Center for Ecological Environment in Arid Area
| DOI | 10.3390/plants10102005 |
| DNA Barcode Authentication of Devil's Claw Herbal Dietary Supplements | |
| Diaz-Silveira, Genelle L.; Deutsch, Joan; Little, Damon P. | |
| 通讯作者 | Little, DP (corresponding author), New York Bot Garden, Lewis B & Dorothy Cullman Program Mol Systemat, Bronx, NY 10458 USA. |
| 来源期刊 | PLANTS-BASEL
 |
| EISSN | 2223-7747 |
| 出版年 | 2021 |
| 卷号 | 10期号:10 |
| 英文摘要 | Devil's claw is the vernacular name for a genus of medicinal plants that occur in the Kalahari Desert and Namibia Steppes. The genus comprises two distinct species: Harpagophytum procumbens and H. zeyheri. Although the European pharmacopeia considers the species interchangeable, recent studies have demonstrated that H. procumbens and H. zeyheri are chemically distinct and should not be treated as the same species. Further, the sale of H. zeyheri as an herbal supplement is not legal in the United States. Four markers were tested for their ability to distinguish H. procumbens from H. zeyheri: rbcL, matK, nrITS2, and psbA-trnH. Of these, only psbA-trnH was successful. A novel DNA mini-barcode assay that produces a 178-base amplicon in Harpagophytum (specificity = 1.00 [95% confidence interval = 0.80-1.00]; sensitivity = 1.00 [95% confidence interval = 0.75-1.00]) was used to estimate mislabeling frequency in a sample of 23 devil's claw supplements purchased in the United States. PCR amplification failed in 13% of cases. Among the 20 fully-analyzable supplements: H. procumbens was not detected in 75%; 25% contained both H. procumbens and H. zeyheri; none contained only H. procumbens. We recommend this novel mini-barcode region as a standard method of quality control in the manufacture of devil's claw supplements. |
| 英文关键词 | Harpagophytum procumbens Harpagophytum zeyheri mini-barcode Pedaliaceae psbA-trnH |
| 类型 | Article |
| 语种 | 英语 |
| 开放获取类型 | Green Published, gold |
| 收录类别 | SCI-E |
| WOS记录号 | WOS:000713192800001 |
| WOS关键词 | REAL-TIME PCR ; HARPAGOPHYTUM-PROCUMBENS ; MAIZE ; AMPLIFICATION ; DEGRADATION ; EXTRACT ; BREAD ; WHEAT ; CYCLOOXYGENASE-2 ; IDENTIFICATION |
| WOS类目 | Plant Sciences |
| WOS研究方向 | Plant Sciences |
| 资源类型 | 期刊论文 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/368390 |
| 作者单位 | [Diaz-Silveira, Genelle L.; Deutsch, Joan; Little, Damon P.] New York Bot Garden, Lewis B & Dorothy Cullman Program Mol Systemat, Bronx, NY 10458 USA |
| 推荐引用方式 GB/T 7714 | Diaz-Silveira, Genelle L.,Deutsch, Joan,Little, Damon P.. DNA Barcode Authentication of Devil's Claw Herbal Dietary Supplements[J],2021,10(10). |
| APA | Diaz-Silveira, Genelle L.,Deutsch, Joan,&Little, Damon P..(2021).DNA Barcode Authentication of Devil's Claw Herbal Dietary Supplements.PLANTS-BASEL,10(10). |
| MLA | Diaz-Silveira, Genelle L.,et al."DNA Barcode Authentication of Devil's Claw Herbal Dietary Supplements".PLANTS-BASEL 10.10(2021). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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