Arid
DOI10.3390/plants10102005
DNA Barcode Authentication of Devil's Claw Herbal Dietary Supplements
Diaz-Silveira, Genelle L.; Deutsch, Joan; Little, Damon P.
通讯作者Little, DP (corresponding author), New York Bot Garden, Lewis B & Dorothy Cullman Program Mol Systemat, Bronx, NY 10458 USA.
来源期刊PLANTS-BASEL
EISSN2223-7747
出版年2021
卷号10期号:10
英文摘要Devil's claw is the vernacular name for a genus of medicinal plants that occur in the Kalahari Desert and Namibia Steppes. The genus comprises two distinct species: Harpagophytum procumbens and H. zeyheri. Although the European pharmacopeia considers the species interchangeable, recent studies have demonstrated that H. procumbens and H. zeyheri are chemically distinct and should not be treated as the same species. Further, the sale of H. zeyheri as an herbal supplement is not legal in the United States. Four markers were tested for their ability to distinguish H. procumbens from H. zeyheri: rbcL, matK, nrITS2, and psbA-trnH. Of these, only psbA-trnH was successful. A novel DNA mini-barcode assay that produces a 178-base amplicon in Harpagophytum (specificity = 1.00 [95% confidence interval = 0.80-1.00]; sensitivity = 1.00 [95% confidence interval = 0.75-1.00]) was used to estimate mislabeling frequency in a sample of 23 devil's claw supplements purchased in the United States. PCR amplification failed in 13% of cases. Among the 20 fully-analyzable supplements: H. procumbens was not detected in 75%; 25% contained both H. procumbens and H. zeyheri; none contained only H. procumbens. We recommend this novel mini-barcode region as a standard method of quality control in the manufacture of devil's claw supplements.

英文关键词Harpagophytum procumbens Harpagophytum zeyheri mini-barcode Pedaliaceae psbA-trnH
类型Article
语种英语
开放获取类型Green Published, gold
收录类别SCI-E
WOS记录号WOS:000713192800001
WOS关键词REAL-TIME PCR ; HARPAGOPHYTUM-PROCUMBENS ; MAIZE ; AMPLIFICATION ; DEGRADATION ; EXTRACT ; BREAD ; WHEAT ; CYCLOOXYGENASE-2 ; IDENTIFICATION
WOS类目Plant Sciences
WOS研究方向Plant Sciences
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/368390
作者单位[Diaz-Silveira, Genelle L.; Deutsch, Joan; Little, Damon P.] New York Bot Garden, Lewis B & Dorothy Cullman Program Mol Systemat, Bronx, NY 10458 USA
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GB/T 7714
Diaz-Silveira, Genelle L.,Deutsch, Joan,Little, Damon P.. DNA Barcode Authentication of Devil's Claw Herbal Dietary Supplements[J],2021,10(10).
APA Diaz-Silveira, Genelle L.,Deutsch, Joan,&Little, Damon P..(2021).DNA Barcode Authentication of Devil's Claw Herbal Dietary Supplements.PLANTS-BASEL,10(10).
MLA Diaz-Silveira, Genelle L.,et al."DNA Barcode Authentication of Devil's Claw Herbal Dietary Supplements".PLANTS-BASEL 10.10(2021).
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