干旱区生态环境知识资源中心(Arid): Determination of Pyrrolizidine Alkaloids in Teas Using Liquid Chromatography-Tandem Mass Spectrometry Combined with Rapid-Easy Extraction

Arid

DOI	10.3390/foods10102250
	Determination of Pyrrolizidine Alkaloids in Teas Using Liquid Chromatography-Tandem Mass Spectrometry Combined with Rapid-Easy Extraction
	Kwon, Yujihn; Koo, Yongeui; Jeong, Yoonhwa
通讯作者	Jeong, Y (corresponding author), Dankook Univ, Dept Food Sci & Nutr, 119 Dandaero, Cheonan Si 31116, South Korea.
来源期刊	FOODS
EISSN	2304-8158
出版年	2021
卷号	10 期号:10
英文摘要	This study developed an analytical method to determine pyrrolizidine alkaloids (PAs) in teas using liquid chromatography-tandem mass spectrometry combined with rapid-easy extraction. PAs were extracted with 40 mL of 0.05 M sulfuric acid in 50% methanol solution and cleaned up using Oasis MCX SPE cartridges. Chromatographic separation of 21 PAs was conducted on an X-Bridge C18 column with gradient elution. According to the AOAC official analysis methods, the developed method was verified to establish linearity, limits of detection, limits of quantification, accuracy, inter-day precision, and intra-day precision for each PA. Overall, the method showed excellent repeatability, sensitivity, and reproducibility. The verified method was applied to tea samples, including mate, lemon balm, fennel, hibiscus, chrysanthemum, lavender, oolong tea, chamomile, rooibos, peppermint, mix tea, black, and green tea. One of the main advantages of the method developed in this study is that it allows complete separation of lycopsamine and intermedine peaks. Therefore, the method could be used to monitor PAs in teas.
英文关键词	pyrrolizidine alkaloids tea natural toxins LC-MS/MS & nbsp SPE
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000716981600001
WOS关键词	QUANTIFICATION ; PLANTS
WOS类目	Food Science & Technology
WOS研究方向	Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/368308
作者单位	[Kwon, Yujihn; Jeong, Yoonhwa] Dankook Univ, Dept Food Sci & Nutr, 119 Dandaero, Cheonan Si 31116, South Korea; [Koo, Yongeui] Minist Food & Drug Safety, Natl Food & Drug Safety Evaluat, Food Safety Evaluat Dept, Food Contaminants Div, Cheongju 28159, South Korea
推荐引用方式 GB/T 7714	Kwon, Yujihn,Koo, Yongeui,Jeong, Yoonhwa. Determination of Pyrrolizidine Alkaloids in Teas Using Liquid Chromatography-Tandem Mass Spectrometry Combined with Rapid-Easy Extraction[J],2021,10(10).
APA	Kwon, Yujihn,Koo, Yongeui,&Jeong, Yoonhwa.(2021).Determination of Pyrrolizidine Alkaloids in Teas Using Liquid Chromatography-Tandem Mass Spectrometry Combined with Rapid-Easy Extraction.FOODS,10(10).
MLA	Kwon, Yujihn,et al."Determination of Pyrrolizidine Alkaloids in Teas Using Liquid Chromatography-Tandem Mass Spectrometry Combined with Rapid-Easy Extraction".FOODS 10.10(2021).