干旱区生态环境知识资源中心(Arid): Saccharopolyspora karakumensis sp. nov., Saccharopolyspora elongata sp. nov., Saccharopolyspora aridisoli sp. nov., Saccharopolyspora terrae sp. nov. and their biotechnological potential revealed by genome analysis

Arid

DOI	10.1016/j.syapm.2021.126270
	Saccharopolyspora karakumensis sp. nov., Saccharopolyspora elongata sp. nov., Saccharopolyspora aridisoli sp. nov., Saccharopolyspora terrae sp. nov. and their biotechnological potential revealed by genome analysis
	Saygin, Hayrettin; Ay, Hilal; Guven, Kiymet; Inan-Bektas, Kadriye; Cetin, Demet; Sahin, Nevzat
通讯作者	Sahin, N (corresponding author), Ondokuz Mayis Univ, Fac Sci & Arts, Dept Mol Biol & Genet, TR-55139 Samsun, Turkey.
来源期刊	SYSTEMATIC AND APPLIED MICROBIOLOGY
ISSN	0723-2020
EISSN	1618-0984
出版年	2021
卷号	44 期号:6
英文摘要	Exploration of unexplored habitats for novel actinobacteria with high bioactivity potential holds great promise in the search for novel entities. During the course of isolation of actinobacteria from desert soils, four actinobacteria, designated as 5K548T, 7K502T, 16K309T and 16K404T, were isolated from the Karakum Desert and their bioactivity potential as well as taxonomic provenances were revealed by com-prehensive genome analyses. Pairwise sequence analyses of the 16S rRNA genes indicated that the four strains are representatives of putatively novel taxa within the prolific actinobacterial genus Saccharopolyspora. The strains have typical chemotaxonomic characteristics of the genus Saccharopolyspora by having meso-diaminopimelic acid as diagnostic diaminoacid, arabinose, galactose and ribose as whole-cell sugars. Consistent with this assignment, all of the isolates contained phos-phatidylcholine in their polar lipid profiles and MK-9(H4) as the predominant menaquinone. The sizes of the genomes of the isolates ranged from 6.0 to 10.2 Mb and the associated G + C contents from 69.6 to 69.7 %. Polyphasic characterizations including determination of overall genome relatedness indices revealed that the strains are representatives of four novel species in the genus Saccharopolyspora. Consequently, isolates 5K548T, 7K502T, 16K404T and 16K309T are proposed as novel Saccharopolyspora species for which the names of Saccharopolyspora karakumensis sp. nov., Saccharopolyspora elongata sp. nov., Saccharopolyspora aridisoli sp. nov. and Saccharopolyspora terrae sp. nov. are proposed, respectively. Comprehensive genome analysis for biosynthetic gene clusters showed that the strains have high poten-tial for novel secondary metabolites. Moreover, the strains harbour many antimicrobial resistance genes providing more evidence for their potentiality for bioactive metabolites. (c) 2021 Elsevier GmbH. All rights reserved.
英文关键词	Actinobacteria Antimicrobial resistance genes Biosynthetic gene clusters Bioactivity Desert
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000711349900004
WOS关键词	DNA HYBRIDIZATION ; HUMIC-ACID ; DESERT ; STREPTOMYCES ; ACTINOBACTERIA ; CLASSIFICATION ; IDENTIFICATION ; ACTINOMYCETE ; METABOLITES ; DIVERSITY
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/368240
作者单位	[Saygin, Hayrettin; Ay, Hilal; Sahin, Nevzat] Ondokuz Mayis Univ, Fac Sci & Arts, Dept Mol Biol & Genet, TR-55139 Samsun, Turkey; [Saygin, Hayrettin] Ondokuz Mayis Univ, Fac Sci & Arts, Dept Biol, TR-55139 Samsun, Turkey; [Guven, Kiymet] Eskisehir Tech Univ, Fac Sci, Dept Biol, TR-26555 Eskisehir, Turkey; [Inan-Bektas, Kadriye] Karadeniz Tech Univ, Fac Sci, Dept Mol Biol & Genet, TR-61080 Trabzon, Turkey; [Cetin, Demet] Gazi Univ, Dept Math & Sci Educ, Div Sci Educ, TR-06500 Ankara, Turkey
推荐引用方式 GB/T 7714	Saygin, Hayrettin,Ay, Hilal,Guven, Kiymet,et al. Saccharopolyspora karakumensis sp. nov., Saccharopolyspora elongata sp. nov., Saccharopolyspora aridisoli sp. nov., Saccharopolyspora terrae sp. nov. and their biotechnological potential revealed by genome analysis[J],2021,44(6).
APA	Saygin, Hayrettin,Ay, Hilal,Guven, Kiymet,Inan-Bektas, Kadriye,Cetin, Demet,&Sahin, Nevzat.(2021).Saccharopolyspora karakumensis sp. nov., Saccharopolyspora elongata sp. nov., Saccharopolyspora aridisoli sp. nov., Saccharopolyspora terrae sp. nov. and their biotechnological potential revealed by genome analysis.SYSTEMATIC AND APPLIED MICROBIOLOGY,44(6).
MLA	Saygin, Hayrettin,et al."Saccharopolyspora karakumensis sp. nov., Saccharopolyspora elongata sp. nov., Saccharopolyspora aridisoli sp. nov., Saccharopolyspora terrae sp. nov. and their biotechnological potential revealed by genome analysis".SYSTEMATIC AND APPLIED MICROBIOLOGY 44.6(2021).