干旱区生态环境知识资源中心(Arid): Combined Molecular and Elemental Mass Spectrometry Approaches for Absolute Quantification of Proteomes: Application to the Venomics Characterization of the Two Species of Desert Black Cobras, Walterinnesia aegyptia and Walterinnesia morgani

Arid

DOI	10.1021/acs.jproteome.1c00608
	Combined Molecular and Elemental Mass Spectrometry Approaches for Absolute Quantification of Proteomes: Application to the Venomics Characterization of the Two Species of Desert Black Cobras, Walterinnesia aegyptia and Walterinnesia morgani
	Calvete, Juan J.; Pla, Davinia; Els, Johannes; Carranza, Salvador; Damm, Maik; Hempel, Benjamin-Florian; John, Elisa B. O.; Petras, Daniel; Heiss, Paul; Nalbantsoy, Ayse; Gocmen, Bayram; Suessmuth, Roderich D.; Calderon-Celis, Francisco; Nosti, Alicia Jimenez; Ruiz Encinar, Jorge
通讯作者	Calvete, JJ (corresponding author), CSIC, Inst Biomed Valencia, Lab Venom Evolut & Traslat, Valencia 46010, Spain.
来源期刊	JOURNAL OF PROTEOME RESEARCH
ISSN	1535-3893
EISSN	1535-3907
出版年	2021
卷号	20 期号:11 页码:5064-5078
英文摘要	We report a novel hybrid, molecular and elemental mass spectrometry (MS) setup for the absolute quantification of snake venom proteomes shown here for two desert black cobra species within the genus Walterinnesia, Walterinnesia aegyptia and Walterinnesia morgani. The experimental design includes the decomplexation of the venom samples by reverse-phase chromatography independently coupled to four mass spectrometry systems: the combined bottom-up and top-down molecular MS for protein identification and a parallel reverse-phase microbore high-performance liquid chromatograph (RP-mu HPLC) on-line to inductively coupled plasma (ICP-MS/MS) elemental mass spectrometry and electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QToF MS). This allows to continuously record the absolute sulfur concentration throughout the chromatogram and assign it to the parent venom proteins separated in the RP-mu HPLC-ESI-QToF parallel run via mass profiling. The results provide a locus-resolved and quantitative insight into the three desert black cobra venom proteome samples. They also validate the units of measure of our snake venomics strategy for the relative quantification of snake venom proteomes as % of total venom peptide bonds as a proxy for the % by weight of the venom toxins/toxin families. In a more general context, our work may pave the way for broader applications of hybrid elemental/molecular MS setups in diverse areas of proteomics.
英文关键词	snake venomics combined top-down and bottom-up venomics hybrid elemental and molecular mass spectrometry absolute quantification of venom proteome desert black cobra Walterinnesia aegyptia Walterinnesia morgani
类型	Article
语种	英语
开放获取类型	hybrid
收录类别	SCI-E
WOS记录号	WOS:000716799900014
WOS关键词	TOP-DOWN VENOMICS ; PEPTIDE DETECTABILITY ; SNAKE VENOMICS ; ICP-MS ; PROTEINS ; IDENTIFICATION ; BIOMOLECULES ; STANDARDS ; ELAPIDAE ; OPHIDIA
WOS类目	Biochemical Research Methods
WOS研究方向	Biochemistry & Molecular Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/368075
作者单位	[Calvete, Juan J.; Pla, Davinia] CSIC, Inst Biomed Valencia, Lab Venom Evolut & Traslat, Valencia 46010, Spain; [Els, Johannes] Environm & Protected Areas Author, Sharjah 82828, U Arab Emirates; [Carranza, Salvador] Univ Pompeu Fabra, Inst Evolutionary Biol, CSIC, Barcelona 08003, Spain; [Damm, Maik; Hempel, Benjamin-Florian; Heiss, Paul; Suessmuth, Roderich D.] Tech Univ Berlin, Dept Chem, D-10623 Berlin, Germany; [Hempel, Benjamin-Florian] Charite Univ Med Berlin, BIH Ctr Regenerat Therapies BCRT, D-13353 Berlin, Germany; [John, Elisa B. O.] Univ Fed Rio Grande do Sul, Ctr Biotechnol, BR-91501970 Porto Alegre, RS, Brazil; [Petras, Daniel] Univ Tubingen, Interfac Inst Microbiol & Med, CMFI Cluster Excellence, D-72076 Tubingen, Germany; [Nalbantsoy, Ayse] Ege Univ, Fac Engn, Dept Bioengn, TR-35100 Bornova, Turkey; [Gocmen, Bayram] Ege Univ, Fac Sci, Dept Biol, Zool Sect, TR-35100 Bornova, Turkey; [Calderon-Celis, Francisco; Nosti, Alicia Jimenez; Ruiz Encinar, Jorge] Univ Oviedo, Dept Phys & Analyt Chem, ...
推荐引用方式 GB/T 7714	Calvete, Juan J.,Pla, Davinia,Els, Johannes,et al. Combined Molecular and Elemental Mass Spectrometry Approaches for Absolute Quantification of Proteomes: Application to the Venomics Characterization of the Two Species of Desert Black Cobras, Walterinnesia aegyptia and Walterinnesia morgani[J],2021,20(11):5064-5078.
APA	Calvete, Juan J..,Pla, Davinia.,Els, Johannes.,Carranza, Salvador.,Damm, Maik.,...&Ruiz Encinar, Jorge.(2021).Combined Molecular and Elemental Mass Spectrometry Approaches for Absolute Quantification of Proteomes: Application to the Venomics Characterization of the Two Species of Desert Black Cobras, Walterinnesia aegyptia and Walterinnesia morgani.JOURNAL OF PROTEOME RESEARCH,20(11),5064-5078.
MLA	Calvete, Juan J.,et al."Combined Molecular and Elemental Mass Spectrometry Approaches for Absolute Quantification of Proteomes: Application to the Venomics Characterization of the Two Species of Desert Black Cobras, Walterinnesia aegyptia and Walterinnesia morgani".JOURNAL OF PROTEOME RESEARCH 20.11(2021):5064-5078.