干旱区生态环境知识资源中心(Arid): Chemical characterization and evaluation of the neuroprotective potential of Indigofera sessiliflora through in-silico studies and behavioral tests in scopolamine-induced memory compromised rats

Arid

DOI	10.1016/j.sjbs.2021.04.033
	Chemical characterization and evaluation of the neuroprotective potential of Indigofera sessiliflora through in-silico studies and behavioral tests in scopolamine-induced memory compromised rats
	Haider, Muhammad Sajjad; Ashraf, Waseem; Javaid, Sana; Rasool, Muhammad Fawad; Rahman, Hafiz Muhammad Abdur; Saleem, Hammad; Anjum, Syed Muhammad Muneeb; Siddique, Farhan; Morales-Bayuelo, Alejandro; Kaya, Savas; Alqahtani, Faleh; Alasmari, Fawaz; Imran, Imran
通讯作者	Imran, I (corresponding author), Bahauddin Zakariya Univ, Dept Pharmacol, Fac Pharm, Multan 60800, Pakistan. ; Alqahtani, F (corresponding author), King Saud Univ, Dept Pharmacol & Toxicol, Coll Pharm, Riyadh 11451, Saudi Arabia.
来源期刊	SAUDI JOURNAL OF BIOLOGICAL SCIENCES
ISSN	1319-562X
EISSN	2213-7106
出版年	2021
卷号	28 期号:8 页码:4384-4398
英文摘要	In the current study, we investigated the phytochemical and neuropharmacological potential of Indigofera sessiliflora, an indigenous least characterized plant widely distributed in deserted areas of Pakistan. The crude extract of the whole plant Indigofera sessiliflora (IS.CR) was preliminary tested in-vitro for the existence of polyphenol content, antioxidant and anticholinesterase potential followed by detailed chemical characterization through UHPLC-MS. Rats administered with different doses of IS.CR (100-300 mg/kg) for the duration of 4-weeks were behaviorally tested for anxiety and cognition followed by biochemical evaluation of dissected brain. The in-silico studies were employed to predict the blood-brain barrier crossing tendencies of secondary metabolites with the elucidation of the target binding site. The in-vitro assays revealed ample phenols and flavonoids content in IS.CR with adequate anti-oxidant and anti cholinesterase potential. The dose-dependent anxiolytic potential of IS.CR was demonstrated in open field (OFT), light/dark (L/D) and elevated plus maze (EPM) tests as animals spent more time in open, illuminated and elevated zones (P < 0.05). In the behavioral tests for learning/memory, the IS.CR reversed the scopolamine-induced cognitive deficits, as animals showed better (P < 0.05) spontaneous alternation and discrimination index in y-maze and novel object recognition (NOR) tests. Similarly, as compared to amnesic rats, the step-through latencies were increased (P < 0.05) and escape latencies were decreased (P < 0.05) in passive avoidance (PAT) and Morris water maze (MWM) tests, respectively. Biochemical analysis of rat brains showed significant reduction in malondialdehyde and acetylcholinesterase levels, alongwith preservation of glutathione peroxidase and superoxide dismutase activity. The docking studies further portrayed a possible interaction of detected phytoconstituents with acetylcholinesterase target. The results of the study show valuable therapeutic potential of phytoconstituents present in IS.CR to correct the neurological disarrays which might be through antioxidant activity or via modulation of GABAergic and cholinergic systems by artocommunol, 1,9-dideoxyforskolin and 6E,9E-octadecadienoic acid. (c) 2021 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
英文关键词	Indigofera sessiliflora Artocommunol Anxiety Learning memory Behavioral studies
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000678372600005
WOS关键词	OXIDATIVE STRESS ; AQUEOUS EXTRACT ; WATER MAZE ; ANTIOXIDANT ; ACETYLCHOLINESTERASE ; IMPAIRMENT ; FORSKOLIN ; PROTEIN ; MODEL ; DOCKING
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
来源机构	King Saud University
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/368072
作者单位	[Haider, Muhammad Sajjad; Ashraf, Waseem; Javaid, Sana; Rahman, Hafiz Muhammad Abdur; Imran, Imran] Bahauddin Zakariya Univ, Dept Pharmacol, Fac Pharm, Multan 60800, Pakistan; [Javaid, Sana] Women Univ, Dept Pharm, Multan 60000, Pakistan; [Rasool, Muhammad Fawad] Bahauddin Zakariya Univ, Dept Pharm Practice, Fac Pharm, Multan 60800, Pakistan; [Saleem, Hammad; Anjum, Syed Muhammad Muneeb] Univ Vet & Anim Sci, Inst Pharmaceut Sci, Lahore, Pakistan; [Siddique, Farhan] Bahauddin Zakariya Univ, Dept Pharmaceut Chem, Fac Pharm, Multan 60800, Pakistan; [Siddique, Farhan] Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin 300072, Peoples R China; [Morales-Bayuelo, Alejandro] Fdn Univ Tecnol, Fac Ingn, Ctr Invest Proc Tecnol Comfenalco, CIPTEC,Programa Ingn Ind, Bolivar, Colombia; [Kaya, Savas] Sivas Cumhuriyet Univ, Hlth Serv Vocat Sch, Dept Pharm, TR-8140 Sivas, Turkey; [Alqahtani, Faleh; Alasmari, Fawaz] King Saud Univ, Dept Pharmacol & Toxicol, Coll Pharm, Riyadh 11451, Saudi Arabia
推荐引用方式 GB/T 7714	Haider, Muhammad Sajjad,Ashraf, Waseem,Javaid, Sana,et al. Chemical characterization and evaluation of the neuroprotective potential of Indigofera sessiliflora through in-silico studies and behavioral tests in scopolamine-induced memory compromised rats[J]. King Saud University,2021,28(8):4384-4398.
APA	Haider, Muhammad Sajjad.,Ashraf, Waseem.,Javaid, Sana.,Rasool, Muhammad Fawad.,Rahman, Hafiz Muhammad Abdur.,...&Imran, Imran.(2021).Chemical characterization and evaluation of the neuroprotective potential of Indigofera sessiliflora through in-silico studies and behavioral tests in scopolamine-induced memory compromised rats.SAUDI JOURNAL OF BIOLOGICAL SCIENCES,28(8),4384-4398.
MLA	Haider, Muhammad Sajjad,et al."Chemical characterization and evaluation of the neuroprotective potential of Indigofera sessiliflora through in-silico studies and behavioral tests in scopolamine-induced memory compromised rats".SAUDI JOURNAL OF BIOLOGICAL SCIENCES 28.8(2021):4384-4398.