干旱区生态环境知识资源中心(Arid): Molecular identification and phylogenetic analysis of Trichoderma isolates obtained from woody plants of the semi-arid of Northeast Brazil

Arid

DOI	10.1127/nova_hedwigia/2021/0622
	Molecular identification and phylogenetic analysis of Trichoderma isolates obtained from woody plants of the semi-arid of Northeast Brazil
	Silva, Helane Franca; da Costa, Elaine Martins; Goncalves Santos, Alice Maria; Tenorio do Amaral, Ana Claudia; Vilela de Oliveira, Rafael Jose; Bezerra, Jose Luiz; Martins Newman Luz, Edna Dora
通讯作者	Silva, HF (corresponding author), Univ Fed Piaui, Campus Prof Cinobelina Elvas, BR-64900000 Bom Jesus, Piaui, Brazil.
来源期刊	NOVA HEDWIGIA
ISSN	0029-5035
EISSN	2363-7188
出版年	2021
卷号	112 期号:3-4 页码:485-500
英文摘要	The objective of this study was to perform the molecular identification and phylogenetic analysis of 19 Trichoderma isolates, obtained from surface-disinfected leaves of forest species, in a Cerrado-Caatinga ecotone, in the semiarid region of Northeast Brazil. The DNA regions studied in the experiment were the genes for the translation elongation factor (tef1) and the second largest RNA polymerase subunit (rpb2). The sequences of each gene and the concatenated ones (tef1 + rpb2) were aligned with those of the most similar Trichoderma isolates available in GenBank for the construction of phylogenetic trees. The sequences obtained from the two studied gene regions allowed the identification of 14 isolates within three species of Trichoderma (T. orientale - seven isolates; T. koningiopsis - six isolates and T. longibrachiatum - one isolate) and for five isolates, it was not possible to identify them at the species level. In some cases, the PCR success rate was sufficient for only one of both genes, or both genes were not sufficient for resolution of the species, or hitherto undescribed species were involved. This is the first report of these fungal species in woody forest plants from four botanical families: Fabaceae (Bauhinia cheilantha, Cenostigma macrophyllum, Diptychandra aurantiaca, Mimosa tenuiflora and Pityrocarpa moniliformis), Myrtaceae (Myrcia tomentosa), Combretaceae (Combretum glaucocarpum and Combretum laxum) and Boraginaceae (Cordia toqueve). The high occurrence of native Trichoderma isolates reveals the potential for mycological studies in plants from the semiarid region of Northeast Brazil. In spite of our limited data, comparison with the literature indicated that in the investigated area T. orientale may show a preference towards leaf substrates compared to twigs, that T. longibrachiatum may be more commonly associated with Fabaceae than with other plants, whereas T. koningiopsis is a widespread unspecific species with a broad range of plant substrates.
英文关键词	Cerrado-Caatinga ecotone filamentous fungi forest plants rpb2 gene tef1 gene
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000649561400014
WOS关键词	HARZIANUM SPECIES COMPLEX ; BIOCONTROL ; PRIMER ; SPP.
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/367928
作者单位	[Silva, Helane Franca; da Costa, Elaine Martins; Goncalves Santos, Alice Maria] Univ Fed Piaui, Campus Prof Cinobelina Elvas, BR-64900000 Bom Jesus, Piaui, Brazil; [Tenorio do Amaral, Ana Claudia; Vilela de Oliveira, Rafael Jose] Univ Fed Pernambuco, Biosci Ctr, Dept Mycol, BR-50670901 Recife, PE, Brazil; [Bezerra, Jose Luiz; Martins Newman Luz, Edna Dora] Execut Commiss Cocoa Cultivat Planning, Phytopathol Sect, Cocoa Res Ctr, BR-45660000 Ilheus, BA, Brazil
推荐引用方式 GB/T 7714	Silva, Helane Franca,da Costa, Elaine Martins,Goncalves Santos, Alice Maria,et al. Molecular identification and phylogenetic analysis of Trichoderma isolates obtained from woody plants of the semi-arid of Northeast Brazil[J],2021,112(3-4):485-500.
APA	Silva, Helane Franca.,da Costa, Elaine Martins.,Goncalves Santos, Alice Maria.,Tenorio do Amaral, Ana Claudia.,Vilela de Oliveira, Rafael Jose.,...&Martins Newman Luz, Edna Dora.(2021).Molecular identification and phylogenetic analysis of Trichoderma isolates obtained from woody plants of the semi-arid of Northeast Brazil.NOVA HEDWIGIA,112(3-4),485-500.
MLA	Silva, Helane Franca,et al."Molecular identification and phylogenetic analysis of Trichoderma isolates obtained from woody plants of the semi-arid of Northeast Brazil".NOVA HEDWIGIA 112.3-4(2021):485-500.