干旱区生态环境知识资源中心(Arid): Rapid analysis of polyhydroxyalkanoate contents and its monomer compositions by pyrolysis-gas chromatography combined with mass spectrometry (Py-GC/MS)

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DOI	10.1016/j.ijbiomac.2021.01.108
	Rapid analysis of polyhydroxyalkanoate contents and its monomer compositions by pyrolysis-gas chromatography combined with mass spectrometry (Py-GC/MS)
	Khang, Tae Uk; Kim, Min-Jae; Yoo, Jee In; Sohn, Yu Jung; Jeon, Sang Goo; Park, Si Jae; Na, Jeong-Geol
通讯作者	Na, JG (corresponding author), Sogang Univ, Dept Chem & Biomol Engn, Seoul 04107, South Korea. ; Park, SJ (corresponding author), Ewha Womans Univ, Syst Hlth & Engn Major Grad Sch, Div Chem Engn & Mat Sci, BK21 Plus Program, Seoul 03760, South Korea.
来源期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
EISSN	1879-0003
出版年	2021
卷号	174 页码:449-456
英文摘要	Here, we report an analysis method for determining PHA (polyhydroxyalkanoates) contents and their monomer composition in microbial cells based on pyrolysis gas chromatography combined with mass spectrometry (Py-GC/MS). Various kinds of microbial cells accumulating different PHA contents and monomer compositions were prepared through the cultivation of Ralstonia eutropha and recombinant Escherichia coli. Py-GC/MS could analyse these samples in a short time without complicated pretreatment steps. Characteristic peaks such as 2-butenoic acid, 2-pentenoic add, and hexadecanoic arid regarding PHA compositions and cell components were identified. Considering constituents of cells and ratios of peak areas of dehydrated monomers to hexadecanoic acid, a simple equation for estimation of PHA contents in microbial cells was derived. Also, monomer compositions of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) in R. eutropha could be successfully determined based on peak area of 2-butenoic add and 2-pentenoic acid of Py-GC/MS, which are the corresponding species of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) in PHBV. Correlation of results between GC-FID and Py-GC/MS could be fitted very well. This method shows similar results for the samples obtained from same experimental conditions, allowing rapid and reliable analysis. Py-GC/MS can be a promising tool to rapidly screen PHA-positive strains based on polymer contents along with monomer compositions. (C) 2021 Elsevier B.V. All rights reserved.
英文关键词	Pyrolysis-GC/MS PHB PHBV
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000625633200045
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/367921
作者单位	[Khang, Tae Uk; Na, Jeong-Geol] Sogang Univ, Dept Chem & Biomol Engn, Seoul 04107, South Korea; [Kim, Min-Jae; Jeon, Sang Goo] Korea Inst Energy Res, 152 Gajeong Ro, Daejeon 34129, South Korea; [Yoo, Jee In; Sohn, Yu Jung; Park, Si Jae] Ewha Womans Univ, Syst Hlth & Engn Major Grad Sch, Div Chem Engn & Mat Sci, BK21 Plus Program, Seoul 03760, South Korea
推荐引用方式 GB/T 7714	Khang, Tae Uk,Kim, Min-Jae,Yoo, Jee In,et al. Rapid analysis of polyhydroxyalkanoate contents and its monomer compositions by pyrolysis-gas chromatography combined with mass spectrometry (Py-GC/MS)[J],2021,174:449-456.
APA	Khang, Tae Uk.,Kim, Min-Jae.,Yoo, Jee In.,Sohn, Yu Jung.,Jeon, Sang Goo.,...&Na, Jeong-Geol.(2021).Rapid analysis of polyhydroxyalkanoate contents and its monomer compositions by pyrolysis-gas chromatography combined with mass spectrometry (Py-GC/MS).INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,174,449-456.
MLA	Khang, Tae Uk,et al."Rapid analysis of polyhydroxyalkanoate contents and its monomer compositions by pyrolysis-gas chromatography combined with mass spectrometry (Py-GC/MS)".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 174(2021):449-456.