干旱区生态环境知识资源中心(Arid): Molecular and morphological evaluation of transgenic Persian walnut plants harboring Fld gene under osmotic stress condition

Arid

DOI	10.1007/s11033-021-06893-x
	Molecular and morphological evaluation of transgenic Persian walnut plants harboring Fld gene under osmotic stress condition
	Nazari, Mansoureh; Tohidfar, Masoud; Ramshini, Hossein; Vahdati, Kourosh
通讯作者	Vahdati, K (corresponding author), Univ Tehran, Coll Aburaihan, Dept Hort, Tehran, Iran.
来源期刊	MOLECULAR BIOLOGY REPORTS
ISSN	0301-4851
EISSN	1573-4978
出版年	2022
英文摘要	Background Soil drought stress is a limiting factor of productivity in walnut (Juglans regia L). Ferredoxin (Fd) level decreases under adverse environmental stress. Functional replacement of decreased Fd by Fld (Flavodoxin) had been shown to have protective effect under abiotic stress condition. This study aimed to evaluate four transgenic lines (L3, L4, L13 and L17) along with non-transgenic line under three osmotic stresses levels (0, 10 and 12% PEG). Methods and results This experiment carried out based on a completely randomized design with four replications. To confirm that the Fld gene is successfully integrated into the walnut genome, PCR and dot blot analysis were carried out. The transgenic lines of walnut expressing Fld displayed increased tolerance to osmotic stress at 10 and 12% PEG condition. Lines expressing Fld exhibited increasing tolerance to drought stress and maintained health of plants under osmotic conditions. Results of real time PCR showed that expression level of Fld gene in L4 was higher than the others. Among transgenic lines, L4 was more tolerant than other lines under osmotic stress. Conclusions These findings indicate that expression of Fld gene can increase tolerance to osmotic stress in Persian walnut and is useful tool for walnut production in arid and semi-arid regions.
英文关键词	Flavodoxin (Fld) Juglans regia L Osmotic stress Transgenic plants
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000715011400008
WOS关键词	AGROBACTERIUM-MEDIATED TRANSFORMATION ; CYANOBACTERIAL FLAVODOXIN ; PHOTOSYNTHETIC MICROORGANISMS ; SOMATIC EMBRYOGENESIS ; IRON STARVATION ; WILD-TYPE ; FERREDOXIN ; EXPRESSION ; ACCUMULATION ; TOLERANCE
WOS类目	Biochemistry & Molecular Biology
WOS研究方向	Biochemistry & Molecular Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/367706
作者单位	[Nazari, Mansoureh; Vahdati, Kourosh] Univ Tehran, Coll Aburaihan, Dept Hort, Tehran, Iran; [Tohidfar, Masoud] Shahid Beheshti Univ, Fac Sci & Biotechnol, Dept Plant Biotechnol, Tehran, Iran; [Ramshini, Hossein] Univ Tehran, Coll Aburaihan, Dept Agron & Plant Breeding Sci, Tehran, Iran
推荐引用方式 GB/T 7714	Nazari, Mansoureh,Tohidfar, Masoud,Ramshini, Hossein,et al. Molecular and morphological evaluation of transgenic Persian walnut plants harboring Fld gene under osmotic stress condition[J],2022.
APA	Nazari, Mansoureh,Tohidfar, Masoud,Ramshini, Hossein,&Vahdati, Kourosh.(2022).Molecular and morphological evaluation of transgenic Persian walnut plants harboring Fld gene under osmotic stress condition.MOLECULAR BIOLOGY REPORTS.
MLA	Nazari, Mansoureh,et al."Molecular and morphological evaluation of transgenic Persian walnut plants harboring Fld gene under osmotic stress condition".MOLECULAR BIOLOGY REPORTS (2022).