干旱区生态环境知识资源中心(Arid): Streptomyces blattellae, a novel actinomycete isolated from the in vivo of a Blattella germanica

Arid

DOI	10.1007/s10482-021-01681-w
	Streptomyces blattellae, a novel actinomycete isolated from the in vivo of a Blattella germanica
	Liu, Gui-Min; Yuan, Lin-Lin; Zhang, Li-Li; Zeng, Hong
通讯作者	Zhang, LL (corresponding author), Tarim Univ, Xinjiang Prod & Construct Corps Key Lab Protect &, Coll Life Sci, Alar 843300, Xinjiang, Peoples R China. ; Zeng, H (corresponding author), Youjiang Med Univ Nationalities, Sch Basic Med, Baise 533000, Guangxi, Peoples R China.
来源期刊	ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
ISSN	0003-6072
EISSN	1572-9699
出版年	2022
英文摘要	During a screening for novel and useful actinobacteria in desert animal, a new actinomycete was isolated and designated strain TRM63209(T). The strain was isolated from in vivo of a Blattella germanica in Tarim University in Alar City, Xinjiang, north-west China. The strain was found to exhibit an inhibitory effect on biofilm formation by Candida albicans ATCC 18,804. The strain was observed to form abundant aerial mycelium, occasionally twisted and which differentiated into spiral spore chains. Spores of TRM63209(T) were observed to be oval-shaped, with a smooth surface. Strain TRM63209(T) was found to grow optimally at 28 degrees C, pH 8 and in the presence of 1% (w/v) NaCl. The whole-cell sugars of strain TRM63209(T) were rhamnose ribose, xylose, mannose, galactose and glucose, and the principal polarlipids were found to be diphosphatidylglycerol, phos-phatidylethanolamine, phosphatidylcholine, phosphatidylinositol mannoside, phosphatidylinositol and an unknown phospholipid(L). The diagnostic cell wall amino acid was identified as LL-diaminopimelic acid. The predominant menaquinone was found to be MK-9(H6) (14.64%), MK-9(H2) (19.65%), MK-9(H8) (22.34%), MK-10(H2) (25.37%). The major cellular fatty acids were identified as iso-C16:0, 16:0, anteiso-C15:0, anteiso-C17:0, iso-C15:0 and Sum in Feature 3. Analysis of the 16S rRNA sequence showed that strain TRM63209(T) exhibits high sequence similarity to Streptomyces bungoensis strain DSM 41781(T) 98.20%. A multi-locus sequence analysis of five house-keeping genes (atpD, gyrB, rpoB, recA and trpB) and phylogenomic analysis also illustrated that strain TRM63209(T) should be assigned to the genus Streptomyces. The DNA G + C content of the strain was determined to be 70.2 mol%. Average nucleotide identity (ANI) between strain TRM63209(T) and S. bungoensis DSM 41781(T), Streptomyces phyllanthi PA1-07(T), Streptomyces longwoodensis DSM 41677(T) and Streptomyces caeruleatus NRRL B-24802(T) were 82.76%, 82.54%, 82.65%, 84.02%, respectively. Digtal DNA-DNA (dDDH) hybridization were 26.30%, 25.10%, 26.20%, 29.50%, respectively. Therefore, it is concluded that strain TRM63209(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces blattelae is proposed. The type strain is TRM63209(T) (CCTCC AA 2018093(T) = LMG 31,403 = TRM63209(T)).
英文关键词	Actinomycete Streptomyces blattelae Polyphasic taxonomy Anti-biofilm
类型	Article
语种	英语
开放获取类型	Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000718104700001
WOS关键词	DNA-DNA HYBRIDIZATION ; MULTILOCUS SEQUENCE-ANALYSIS ; SP NOV. ; CLADE ; GENUS
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/367666
作者单位	[Liu, Gui-Min; Yuan, Lin-Lin; Zhang, Li-Li] Tarim Univ, Xinjiang Prod & Construct Corps Key Lab Protect &, Coll Life Sci, Alar 843300, Xinjiang, Peoples R China; [Zeng, Hong] Youjiang Med Univ Nationalities, Sch Basic Med, Baise 533000, Guangxi, Peoples R China
推荐引用方式 GB/T 7714	Liu, Gui-Min,Yuan, Lin-Lin,Zhang, Li-Li,et al. Streptomyces blattellae, a novel actinomycete isolated from the in vivo of a Blattella germanica[J],2022.
APA	Liu, Gui-Min,Yuan, Lin-Lin,Zhang, Li-Li,&Zeng, Hong.(2022).Streptomyces blattellae, a novel actinomycete isolated from the in vivo of a Blattella germanica.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY.
MLA	Liu, Gui-Min,et al."Streptomyces blattellae, a novel actinomycete isolated from the in vivo of a Blattella germanica".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY (2022).