干旱区生态环境知识资源中心(Arid): Quantitative Analysis of Bioactive Carbazole Alkaloids in Murraya koenigii (L.) from Six Different Climatic Zones of India Using UPLC/MS/MS and Their Principal Component Analysis

Arid

DOI	10.1002/cbdv.202100557
	Quantitative Analysis of Bioactive Carbazole Alkaloids in Murraya koenigii (L.) from Six Different Climatic Zones of India Using UPLC/MS/MS and Their Principal Component Analysis
	Nandan, Shiv; Singh, Sumit K.; Singh, Pratibha; Bajpai, Vikas; Mishra, Ashwanee K.; Joshi, Trapti; Mahar, Rohit; Shukla, Sanjeev K.; Mishra, Dipak K.; Kanojiya, Sanjeev
通讯作者	Kanojiya, S (corresponding author), CSIR Cent Drug Res Inst, Sophisticated Analyt Instrument Facil & Res, Lucknow 226031, Uttar Pradesh, India. ; Kanojiya, S (corresponding author), Acad Sci & Innovat Res AcSIR, Ghaziabad 201002, India.
来源期刊	CHEMISTRY & BIODIVERSITY
ISSN	1612-1872
EISSN	1612-1880
出版年	2021
英文摘要	Murraya koenigii (L.) Spreng (Curry leaf) is a commercially important medicinal plant in South Asia, containing therapeutically valuable carbazole alkaloids (CAs). Thus, the quantitative evaluation of these compounds from different climatic zones of India are an important aspect for quality assessment and economic isolation of targeted compounds from the plant. In this study, quantitative estimation of CAs among 34 Indian natural populations of M. koenigii was assessed using UPLC/MS/MS. The collected populations represent the humid subtropical, tropical wet & dry, tropical wet, semi-arid, arid, and montane climatic zones of India. A total of 11 CAs viz. koenine-I, murrayamine A, koenigine, koenimbidine, koenimbine, O-methylmurrayamine A, girinimbine, mahanine, 8,8 ''-biskoenigine, isomahanimbine, and mahanimbine were quantified using multiple reaction monitoring (MRM) experiments within 5.0 min. The respective range for natural abundance of CAs were observed as 0.097-1.222, 0.092-5.014, 0.034-0.661, 0.010-1.673, 0.013-7.336, 0.010-0.310, 0.010-0.114, 0.049-5.288, 0.031-1.731, 0.491-3.791, and 0.492-5.399 mg/g in leaves of M. koenigii. The developed method shown linearity regression coefficient (r(2)>0.9995), LOD (0.003-0.248 ng/mL), LOQ (0.009-0.754 ng/mL), and the recovery was between 88.803-103.729 %. The bulk of these CAs were recorded in their highest concentrations in the humid subtropical zone, followed by the tropical wet & dry zones of India. Further, principal component analysis (PCA) was performed which differentiated the climatic zones according to the dominant and significant CAs contents within the populations. The study concludes that the method established is simple, rapid, with high sample throughput, and can be used as a tool for commercial purposes and quality control of M. koenigii.
英文关键词	Murraya koenigii quantitative analysis principal component analysis carbazole alkaloid climatic zone
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000711551400001
WOS关键词	SPRENG.
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/367601
作者单位	[Nandan, Shiv; Singh, Sumit K.; Singh, Pratibha; Bajpai, Vikas; Mishra, Ashwanee K.; Joshi, Trapti; Mahar, Rohit; Shukla, Sanjeev K.; Mishra, Dipak K.; Kanojiya, Sanjeev] CSIR Cent Drug Res Inst, Sophisticated Analyt Instrument Facil & Res, Lucknow 226031, Uttar Pradesh, India; [Singh, Sumit K.; Singh, Pratibha; Mishra, Dipak K.; Kanojiya, Sanjeev] Acad Sci & Innovat Res AcSIR, Ghaziabad 201002, India
推荐引用方式 GB/T 7714	Nandan, Shiv,Singh, Sumit K.,Singh, Pratibha,et al. Quantitative Analysis of Bioactive Carbazole Alkaloids in Murraya koenigii (L.) from Six Different Climatic Zones of India Using UPLC/MS/MS and Their Principal Component Analysis[J],2021.
APA	Nandan, Shiv.,Singh, Sumit K..,Singh, Pratibha.,Bajpai, Vikas.,Mishra, Ashwanee K..,...&Kanojiya, Sanjeev.(2021).Quantitative Analysis of Bioactive Carbazole Alkaloids in Murraya koenigii (L.) from Six Different Climatic Zones of India Using UPLC/MS/MS and Their Principal Component Analysis.CHEMISTRY & BIODIVERSITY.
MLA	Nandan, Shiv,et al."Quantitative Analysis of Bioactive Carbazole Alkaloids in Murraya koenigii (L.) from Six Different Climatic Zones of India Using UPLC/MS/MS and Their Principal Component Analysis".CHEMISTRY & BIODIVERSITY (2021).