干旱区生态环境知识资源中心(Arid): Most drought-induced DNA methylation changes switched to pre-stress state after re-irrigation in barley (Hordeum vulgare L.) cultivars

Arid

DOI	10.1007/s42976-021-00189-3
	Most drought-induced DNA methylation changes switched to pre-stress state after re-irrigation in barley (Hordeum vulgare L.) cultivars
	Falahi, Ali; Zarei, Leila; Cheghamirza, Kianoosh
通讯作者	Zarei, L (corresponding author), Razi Univ, Fac Sci & Agr Engn, Dept Agron & Plant Breeding, Kermanshah 6715685421, Iran.
来源期刊	CEREAL RESEARCH COMMUNICATIONS
ISSN	0133-3720
EISSN	1788-9170
出版年	2021-06
英文摘要	Drought is the most important factor limiting crop production in arid and semiarid regions of the world. Epigenetic changes including DNA methylation are involved in response to environmental stresses such as drought. In this study, the coupled restriction enzyme digestion-random amplification (CRED-RA) technique was used to evaluate the methylation patterns in leaf of two Iranian barley cultivars Afzal and Sararood and one European barley cultivar Aiace in four moisture stress regimes (irrigation at 100%, 75%, 50% and 25% of field capacity) in three stages including before the application of drought stress, 14 days after stress and 14 days after re-irrigation in three replications. The measured traits were relative water content, photochemical efficiency of photosystem II (Fv/Fm), transpiration rate, sub-stomatal CO2 concentration and leaf surface temperature. The highest methylation changes observed in all three cultivars were the conversion of full methylation to external cytosine methylation. The highest instability of genome pattern was observed in Aiace cultivar and in OPB-07 primer in irrigation at 25% of field capacity. Although most of the methylated and de-methylated fragments switched to pre-stress state after irrigation, there were few fragments that remained stable in the plant's genomic memory. Results showed significant changes in methylation pattern of all three barley cultivars in response to moisture stress.
英文关键词	CRED-RA technique Methylation-sensitive enzymes Moisture stress
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000663481600001
WOS关键词	PLANT ; MANAGEMENT ; GROWTH ; LEAVES
WOS类目	Agronomy
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/367530
作者单位	[Falahi, Ali; Zarei, Leila; Cheghamirza, Kianoosh] Razi Univ, Fac Sci & Agr Engn, Dept Agron & Plant Breeding, Kermanshah 6715685421, Iran
推荐引用方式 GB/T 7714	Falahi, Ali,Zarei, Leila,Cheghamirza, Kianoosh. Most drought-induced DNA methylation changes switched to pre-stress state after re-irrigation in barley (Hordeum vulgare L.) cultivars[J],2021.
APA	Falahi, Ali,Zarei, Leila,&Cheghamirza, Kianoosh.(2021).Most drought-induced DNA methylation changes switched to pre-stress state after re-irrigation in barley (Hordeum vulgare L.) cultivars.CEREAL RESEARCH COMMUNICATIONS.
MLA	Falahi, Ali,et al."Most drought-induced DNA methylation changes switched to pre-stress state after re-irrigation in barley (Hordeum vulgare L.) cultivars".CEREAL RESEARCH COMMUNICATIONS (2021).