干旱区生态环境知识资源中心(Arid): 石漠生境下金银花内生/根际解磷菌在不同温度及酸碱环境下的生长和溶磷能力

Arid

	石漠生境下金银花内生/根际解磷菌在不同温度及酸碱环境下的生长和溶磷能力
其他题名	Growth and Phosphate-solubilizing Ability of Endophytic /Rhizospheric Phosphate-solubilizing Bacteria of Lonicera japonica at Different Temperature and pH Environment in Rocky Desertification Habitat
	李剑峰; 张淑卿; 龙莹; 郭金梅
来源期刊	西南农业学报
ISSN	1001-4829
出版年	2021
卷号	34 期号:4 页码:1001-4829
中文摘要	【目的】从金银花中分离、筛选适宜于石漠化环境的解磷效率高、抗逆性强的菌株应用于石漠化生境的植物解磷菌株。【方法】以贵州省重度石漠化坡地上的金银花(Lonicera japonica)植株及根际土壤中分离出的解磷菌株为供试菌株,模拟石漠地区常见的不同酸碱及温度环境对菌株进行培养,探究酸碱及温度环境对菌株难溶性有机磷和无机磷解磷能力的影响。【结果】在金银花植株及根际土壤中共筛选出7株解磷菌,菌株低于4 ℃均不能生长,在16 ~ 34 ℃能够生长并溶解磷,溶磷能力随温度的升高呈先增后减趋势,但不同菌株最高解磷能力所需求的环境并不一致; 7株解磷菌均能在pH 6.0 ~ 10.0生长,其中PMN1513、 PMN1502、PMN1504和PMN1505在pH 13.0的环境下能够生长并溶解难溶性无机磷;不同分离部位解磷菌解磷耐逆能力为须根内生解磷菌>主根内生解磷菌>根际土壤解磷菌。【结论】分离自金银花茎部的内生解磷菌抗逆及解磷能力普遍强于根际土壤中的解磷菌; pH为6.0的环境下,解磷菌PMN1513的解磷能力最强,对无机磷和有机磷的溶磷指数分别达到2.19和2.72,可用于石漠退化生境中专用解磷菌剂的研发,具有良好的应用潜力。
英文摘要	【Objective】The phosphate-solubilizing efficiency and high resistance to stress of strain suitable for desertification environment is isolated and screened from Lonicera japonica,which is applied to plant phosphate-solubilizing strain in rocky desertification habitat. 【Method 】Phosphate-solubilizing strain isolated from plant and rhizospheric soil of L. japonica growing in serious rocky desertification slope in Guizhou province are taken as tested strains. The strain is cultured at simulated different pH and temperature environment of rocky desertification area to discuss the effects of pH and temperature environment on phosphate-solubilizing ability of insoluble organic phosphate and inorganic phosphate in strain. 【Result】Seven phosphate-solubilizing strains were screened from plant and rhizospheric soil of L. japonica. The strains could not grow below 4 ℃ and could grow at 16 - 34 ℃ and solubilize phosphate. The phosphate-solubilizing ability showed a first increasing and then decreasing trend with the increase of temperature,but the required environment for the maximum phosphate-solubilizing ability of different strains could not be consistent. Seven phosphate-solubilizing strains grew at pH 6.0 - 10.0,PMN1513,PMN1502,PMN1504 and PMN1505 could grow at pH 13.0 and solubilize insoluble inorganic phosphate. The phosphate-solubilizing resistance to stress of phosphate-solubilizing bacteria isolated from different parts was endophytic phosphate-solubilizing bacteria of fibrous root > endophytic phosphate-solubilizing bacteria of main root > phosphate-solubilizing bacteria of rhizospheric soil. 【Conclusion】The resistance to stress and phosphate-solubilizing ability of endophytic phosphate- solubilizing bacteria isolated from L. japonica stem is better than that in phosphate-solubilizing bacteria in rhizospheric soil. PMN1513 has the best phosphate-solubilizing ability at pH 6.0,and the phosphate-solubilizing index to inorganic and organic phosphate reaches 2.19 and 2.7 respectively,which can be used in development of special phosphate-solubilizing bacteria preparation in rocky desertification habitat with a good application potential.
中文关键词	金银花 ; 解磷菌 ; 解磷能力 ; 石漠生境 ; 耐逆性
英文关键词	Lonicera japonica Phosphate-solubilizing bacteria Phosphate-solubilizing ability Rocky desertification habitat Resistance to stress
类型	Article
语种	中文
国家	中国
收录类别	CSCD
WOS类目	Agriculture
CSCD记录号	CSCD:6977211
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/365348
作者单位	李剑峰, 贵州师范学院喀斯特生境土壤与环境生物修复研究所, 贵阳, 贵州 550018, 中国.; 龙莹, 贵州师范学院喀斯特生境土壤与环境生物修复研究所, 贵阳, 贵州 550018, 中国.; 张淑卿, 贵州师范学院喀斯特生境土壤与环境生物修复研究所;;贵州师范学院地理与资源学院, ;;, 贵阳;;贵阳, 贵州;;贵州 550018;;550018, 中国.; 郭金梅, 贵州师范学院喀斯特生境土壤与环境生物修复研究所;;贵州师范学院地理与资源学院, ;;, 贵阳;;贵阳, 贵州;;贵州 550018;;550018, 中国.
推荐引用方式 GB/T 7714	李剑峰,张淑卿,龙莹,等. 石漠生境下金银花内生/根际解磷菌在不同温度及酸碱环境下的生长和溶磷能力[J],2021,34(4):1001-4829.
APA	李剑峰,张淑卿,龙莹,&郭金梅.(2021).石漠生境下金银花内生/根际解磷菌在不同温度及酸碱环境下的生长和溶磷能力.西南农业学报,34(4),1001-4829.
MLA	李剑峰,et al."石漠生境下金银花内生/根际解磷菌在不同温度及酸碱环境下的生长和溶磷能力".西南农业学报 34.4(2021):1001-4829.