干旱区生态环境知识资源中心(Arid): 荒漠草原生物结皮演替对结皮层及层下土壤细菌群落结构的影响

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	荒漠草原生物结皮演替对结皮层及层下土壤细菌群落结构的影响
其他题名	Influence of biocrusts succession process on bacterial community structure of biocrustal layer and subsoils in desert steppe
	樊瑾; 李诗瑶; 王融融; 余海龙; 黄菊莹
来源期刊	生态学杂志
ISSN	1000-4890
出版年	2021
卷号	40 期号:7 页码:1000-4890
中文摘要	为探明生物结皮演替对结皮层及层下土壤细菌群落结构的影响,采用空间代替时间序列的研究方法,以位于宁东能源化工基地的荒漠草原的不同演替阶段(藻结皮(ZB) 、混生结皮(HB)和藓结皮(TB))的生物结皮和其下0 ~ 5 cm土壤为研究对象,通过Illumina NovaSeq PE250平台对细菌16S rRNA的V3-V4高变区进行高通量测序,分析了生物结皮演替过程中结皮层及层下土壤细菌alpha多样性和beta多样性、群落结构及其与土壤理化因子的关系。结果表明:细菌alpha多样性指数表现出了基本一致的趋势,生物结皮下层0~5 cm土壤细菌群落的Chao1指数、ACE指数和香农指数均明显高于结皮层,但不同类型生物结皮细菌 alpha多样性差异不显著(P>0.05);主坐标分析结果表明,不同类型生物结皮下层0 ~ 5 cm土壤中细菌OTUs相近,细菌群落物种组成结构相似;藻结皮和混生结皮细菌群落结构有一定的相似性,而藓结皮与藻结皮、混生结皮差异较大。在门水平上相对丰度最高的5个菌群分别为:放线菌门、变形菌门、酸杆菌门和芽单胞菌门和浮霉菌门,且均表现为下层0 ~ 5 cm土壤的优势菌群相对丰度高于结皮层,下层0 ~ 5 cm土壤的特有属和共有属数量多于结皮层。冗余分析结果表明,土壤有效钾、全氮和有效磷是影响生物结皮层细菌群落结构的主要因素,分别解释了21.4%、18.9%和17.4%的群落结构变化(P<0.05),而EC是影响结皮层下土壤细菌群落结构的主导因子,解释度为20.5%(P<0.05) 。这些结果证实了在生物结皮演替进程中,土壤微生物的发育过程与土壤理化性质的改善相互依存,细菌群落结构组成及多样性的变化可能会促进结皮层理化性质的改善。
英文摘要	To verify the effects of different biological soil crusts (biocrusts) succession phases (algal crusts,mixed crusts,moss crusts) on microbial community compositions and diversities in biocrustal layer and subsoils in a desert steppe,Ningxia,China,the biocrusts and subsoils (0- 5 cm) were sampled using method of space instead of time series. The high-throughput sequencing of V3 -V4 fragment of bacterial 16S rRNA was conducted using the platform of Illumina Miseq. The bacterial alpha and beta diversity,community structure and their relationships with soil physical and chemical factors were analyzed. The results showed that bacterial alpha diversity showed a consistent trend. The Chao1,ACE and Shannon indices in the subsoil (0-5 cm) were all higher than those of biocrustal layer (P<0.05),while alpha diversity among biocrusts was not significant (P>0.05). Results of principal coordinate analysis results indicated that 0-5 cm soil beneath different biocrusts had similar bacterial OTUs,and could be categorized as one type. Bacterial community structures of algal crust and mixed crust had some similarity,but that under moss crust was significantly different from algal crust and mixed crust. The dominant bacteria community of three biocrusts and subsoils were consistent. The top-five dominant bacterial community phyla were Actinobacteria,Proteobacteria,Acidobacteria,Gemmatimonadetes,and Planctomycetes. The relative abundance of these dominant bacterial communities in subsoil (0-5 cm) were higher than that in biocrustal layer,and that of the endemic and shared genera was higher in subsoil (0-5 cm) than in biocrustal layer. Results of distance based redundancy analysis (db-RDA) indicated that soil available potassium,total nitrogen and soil available phosphorus concentrations were the main factors affecting soil bacterial community structure in biocrustal layer,and explained 21.4%,18.9% and 17.4% variances (P<0.05),respectively,whereas EC was the major factor affecting soil bacterial community structure in subsoil (0 - 5 cm),and explained 20.5% of variance (P < 0.05). These results suggested that the development of soil microorganisms and the improvement of soil physical and chemical properties were inter-dependent, and that the changes of bacterial community structure and diversity facilitate the improvement of physical and chemical properties of biocrustal layer during the process of biocrusts succession.
中文关键词	荒漠草原 ; 生物结皮 ; 细菌群落结构 ; 高通量测序
英文关键词	desert steppe biological soil crusts bacterial community structure high-throughput sequencing
类型	Article
语种	中文
国家	中国
收录类别	CSCD
WOS类目	Agriculture
CSCD记录号	CSCD:7010595
来源机构	宁夏大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/365267
作者单位	樊瑾, 宁夏大学地理科学与规划学院, 银川, 宁夏 750021, 中国.; 李诗瑶, 宁夏大学地理科学与规划学院, 银川, 宁夏 750021, 中国.; 王融融, 宁夏大学地理科学与规划学院, 银川, 宁夏 750021, 中国.; 余海龙, 宁夏大学地理科学与规划学院, 银川, 宁夏 750021, 中国.; 黄菊莹, 宁夏大学生态环境学院, 银川, 宁夏 750021, 中国.
推荐引用方式 GB/T 7714	樊瑾,李诗瑶,王融融,等. 荒漠草原生物结皮演替对结皮层及层下土壤细菌群落结构的影响[J]. 宁夏大学,2021,40(7):1000-4890.
APA	樊瑾,李诗瑶,王融融,余海龙,&黄菊莹.(2021).荒漠草原生物结皮演替对结皮层及层下土壤细菌群落结构的影响.生态学杂志,40(7),1000-4890.
MLA	樊瑾,et al."荒漠草原生物结皮演替对结皮层及层下土壤细菌群落结构的影响".生态学杂志 40.7(2021):1000-4890.