干旱区生态环境知识资源中心(Arid): 干旱半干旱煤矿区联合接菌对土壤改良动态生态效应

Arid

	干旱半干旱煤矿区联合接菌对土壤改良动态生态效应
其他题名	Effects of combined inoculation microbes on soil dynamic improvement in coal mining subsidence areas
	毕银丽; 江彬; 秦芳玲; 王茁优
来源期刊	农业工程学报
ISSN	1002-6819
出版年	2021
卷号	37 期号:7 页码:85-93
中文摘要	针对干旱半干旱地区煤炭开采造成的生态破坏问题,利用微生物-植物-土壤的综合作用效应,可加快矿区受损生态的修复进程。为评价不同接种微生物在矿区生态修复过程中的动态作用效果,以神东矿区大柳塔采煤塌陷区的紫穗槐(Amorpha fruticosa L.)为研究对象,通过原位监测,研究接种不同菌根真菌、解磷细菌或同时接种不同微生物处理对不同复垦年限下紫穗槐植株生长(株高和冠幅)、菌根效应(菌根侵染率和菌丝密度)、土壤养分及性质和复垦4 a不同生长季叶片养分和抗逆性的影响规律,以确定接种不同微生物菌剂对紫穗槐生长的影响和对土壤的改良效果。结果表明,与不接种相比,接种摩西管柄囊霉(Funneliformis mosseae)、根内球囊霉(Rhizophagus intraradices)及解磷细菌(Bacillus megaterium)均能显著增加紫穗槐的株高和冠幅(P<0.05),提高其生长旺盛期叶片的氮、磷和钾浓度,提高叶片氮钾的再吸收,降低叶片相对含水率,提高7-9月份叶片脯氨酸、可溶性糖的积累,显著降低土壤pH值(P<0.05),提高土壤易提取球囊霉素、总提取球囊霉素、碱解氮、速效磷和速效钾等指标的含量。不同接种处理对紫穗槐生长、菌根侵染率、叶片抗逆性、土壤氮磷钾养分、易提取球囊霉素和总提取球囊霉素含量的影响随着复垦时间的延长具有显著的协同作用。摩西管柄囊霉与解磷细菌同时接种在复垦4 a后仍能促进紫穗槐菌根效应的发挥,促进植物生长,提高植物的养分和抗逆性,改良土壤,在干旱半干旱煤矿区生态修复进程中具有积极作用。
英文摘要	Deterioration of soil structure has posed a great threat to the farmland and vegetation in ecological and environmental development in large subsided lands,due mainly to excessive coal mining for rapid economic growth in China in recent years.This study aims to accurately and comprehensively evaluate the effects of microorganisms-plants-soil interactions on the soil restoration of damaged ecology caused by coal mining in arid and semi-arid areas.In-situ monitoring was performed on an Amorpha fruticosa L.inoculated by different microorganisms in the coal-mining subsidence area of Daliuta in Shandong Province of eastern China.A systematic evaluation was made on the dynamic ecological effects of inoculated microorganisms (such as mycorrhizal fungi,phosphate solubilizing bacteria or simultaneous inoculation of microorganisms) treatments on the plant growth (plant height and crown width),mycorrhizal effect (mycorrhizal infection rate and hypha density),soil nutrients and properties,the leaf nutrients,and plant resistance under different growth seasons after four years of reclamation in the mining area.A dynamic ecological mechanism of microorganisms was also elucidated in the process of vegetation reclamation in arid and semi-arid coal mine areas.Compared with no inoculation,the inoculation of Funneliformis mosseae,Rhizophagus intraradices,and Bacillus megaterium significantly increased the plant height and crown width of A.fruticosa,as well as the concentration of nitrogen,phosphorus and potassium of A.fruticosa leaves.The reabsorption of leaves and stress resistance of A.fruticosa were also improved remarkably,due possibly to the reduction of relative electrical conductivity and the increase in the accumulation of proline and soluble sugar in the leaves.The soil pH and electrical conductivity were significantly reduced,with an obvious increase in the content of easy-to-extract Glomus(EE-GRSP),total-extract Glomus (T-GRSP),organic carbon,alkali hydrolyzable nitrogen,available phosphorus and available potassium of the rhizosphere soil.There was a significant synergistic effect of inoculation treatments on the growth of A.fruticosa,mycorrhizal infection rate,soil nitrogen,phosphorus and potassium nutrients,EEG and TG content,with the extension of reclamation time.Furthermore,simultaneous inoculation of Funneliformis mosseae and phosphate-dissolving bacteria played a positive role in the plant growth and mycorrhizal effect of A.fruticosa,plant nutrients,and soil stress resistance in arid and semi-arid coal mining areas after 4 years of ecological reclamation.Mycorrhizal fungi were inoculated in the subsided land to maintain sustainable ecological effects.Nevertheless,there was a significant increase in the plant survival/growth rate with a sharp decrease in the nutrient of plant rhizosphere after reclamation about two years.Until four years reclamation,the mycorrhizal fungi with informed hyphal nets contributed to increasing population diversity and absorbing more nutrition from far away space.In natural or managed soil ecosystems,the shifts in the diversity and community structure of arbuscular mycorrhizal fungal (AMF) assemblages over space and time were associated with the plant community succession,anthropogenic activities,and changes in environmental conditions.Such interactions included the mutualistic associations between most vascular plants and their below-ground mycorrhizal symbionts.In any way,the interaction between plant,soil,and mycorrhizal fungi can provide a potential theoretical and practical reference to the restoration of ecological functions and processes in disturbed soil ecosystems,such as post-mining subsided areas.
中文关键词	土壤 ; 养分 ; 采煤塌陷 ; 接菌菌剂 ; 紫穗槐
英文关键词	AMF soils nutrients mining subsidence inoculants Amorpha fruticosa L. arbuscular mycorrhizal fungi
类型	Article
语种	中文
国家	中国
收录类别	CSCD
WOS类目	Environmental Sciences & Ecology
CSCD记录号	CSCD:6997528
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/365180
作者单位	毕银丽, 西安科技大学西部矿山生态环境修复研究院;;中国矿业大学(北京)矿山生态修复研究院, ;;, 西安;;, ;;北京 710054;;100083.; 江彬, 中国矿业大学(北京)矿山生态修复研究院, 北京 100083, 中国.; 秦芳玲, 西安石油大学化学化工学院, 陕西省油气田环境污染与储层保护重点实验室, 西安, 陕西 710065, 中国.; 王茁优, 西安科技大学西部矿山生态环境修复研究院, 西安, 陕西 710054, 中国.
推荐引用方式 GB/T 7714	毕银丽,江彬,秦芳玲,等. 干旱半干旱煤矿区联合接菌对土壤改良动态生态效应[J],2021,37(7):85-93.
APA	毕银丽,江彬,秦芳玲,&王茁优.(2021).干旱半干旱煤矿区联合接菌对土壤改良动态生态效应.农业工程学报,37(7),85-93.
MLA	毕银丽,et al."干旱半干旱煤矿区联合接菌对土壤改良动态生态效应".农业工程学报 37.7(2021):85-93.